Development of highly precise mass spectrometric analytical system for creation of nutrimetabolomics

2011 Fiscal Year Final Research Report

• Project/Area Number: 22658043
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Food science
• Research Institution: Kyushu University
• Principal Investigator: FUJIMURA Yoshinori 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (20390304)
• Co-Investigator(Kenkyū-buntansha): TACHIBANA Hirofumi 九州大学, 大学院・農学研究院, 教授 (70236545) ; MIURA Daisuke 九州大学, 先端融合医療レドックスナビ研究拠点, 准教授 (40532627)
• Project Period (FY): 2010 – 2011
• Keywords: 食品機能 / 緑茶カテキン / ニュートリメタボロミクス / 代謝物 / MALDI-MS / マトリックス / 質量分析イメージング

Research Abstract

Herein, we attempted to establish a novel evaluation method of function of fbod factor based on two-dimensional molecular behavior in local tissuemicro-regions, by developing an advanced MAI. DI-MS system capable of visualizing of bothexoge nous food factors and endogenous metabolites simultaneously. Because MS selecti vityand detection limit against various molecules are dependent on its ionization rate, we performed the screening of matrix, which can aid the ionization of both food factor and endogenous metabolites, and its optimization. The obtained matrix enabled us totwo-dimensionally visualize the green tea catechin EGCG in tissue micro-regions after theoral administration of EGCG and endogenous metabolites in response to EGCG. Theseresults suggest that our proposed MS system may contribute to the establishment of highlyprecise and reliable technique fbr evaluating the potential function of fbod factor in view ofefficacy and safbty.

Research Products

• [Journal Article] In situ metabolomic mass spectrometry imaging : Recent advances and difficulties. J. (2012)
  • Author(s): Miura D, Fujimura Y, and Wariishi H.
  • Journal Title: Proteomics Volume: (in press)
  • Peer Reviewed
• [Journal Article] Metabolomicsdriven nutraceutical evaluation of diverse green tea cultivars (2011)
  • Author(s): Fujimura Y, Kurihara K, Ida M, Kosaka R, Miura D, Wariishi H, Maeda-Yamamoto M, Nesumi A, Saito T, Kanda T, Yamada K, and Tachibana H
  • Journal Title: PLoS ONE Volume: 6 Pages: e23426
  • Peer Reviewed
• [Journal Article] Ultrahighly sensitive in situ metabolomic imaging for visualizing spatiotemporal metabolic behaviors (2010)
  • Author(s): Miura D, Fujimura Y, Yamato M, Hyodo F, Utsumi H, Tachibana H, and Wariishi H
  • Journal Title: Anal. Chem Volume: 82 Pages: 9789-9796
  • Peer Reviewed
• [Journal Article] TLR4 signaling inhibitoxy pathway induced by green tea polyphenol epigallocatechin-3-gallate through 67-kDa laminin receptor. J (2010)
  • Author(s): Byun EH Fujimura Y, Yamada K, and Tachibana H.
  • Journal Title: Immunol Volume: 185 Pages: 33-45
  • Peer Reviewed
• [Presentation] 組織内微小領域における緑茶カテキンの二次元可視化に向けた質量分析イメージング法の開発 (2012)
  • Author(s): 萩原立春,藤村由紀,金允喜,行平大地,山口歩,三浦大典,割石博之,山田耕路,立花宏文.
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都女子大学(京都市)
  • Year and Date: 2012-03-23
• [Presentation] ニュートリメタボロミクスを切り拓く多元的質量分析システムの開発 (2011)
  • Author(s): 藤村由紀.
  • Organizer: 日本農芸化学会西日本支部若手シンポジクムピ(招待講演)
  • Place of Presentation: コテージヒムカ(宮崎市)
  • Year and Date: 2011-09-18
• [Presentation] 食品機能性評価に向けた代謝物プロファイリング技術の開発 (2011)
  • Author(s): 藤村由紀.
  • Organizer: 産学宮連携技術シーズセミナーin福岡(招待講演)
  • Place of Presentation: 福岡市リファレンス駅東ビル(福岡市)
  • Year and Date: 2011-09-01
• [Presentation] 生体低分子のMAHDI-MS測定に有効なマトリックスの開発 (2011)
  • Author(s): 寺井康了,行平大地,三浦大典,藤村由紀,割石博之,新藤充
  • Organizer: 第48回化学衡連支部合同九州大会
  • Place of Presentation: 北九州国際会議場(北九州市)
  • Year and Date: 2011-07-09
• [Presentation] Highthroughput Metabolic Profiling and in situ Metabolite Imaging by MALDI mass spectrometry (2011)
  • Author(s): Miura D, Yukibira D, Fujimura Y, and Wariishi H.
  • Organizer: 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Denver, USA
  • Year and Date: 2011-07-08
• [Presentation] 緑茶ポリフェノールEGCGの抗腫瘍効果に対する酸素応答性の理解に向けた細胞内代謝物プロファイリング (2011)
  • Author(s): 藤村由紀,塚本俊太郎,入江美穂,三浦大典,割石博之,立花宏文.
  • Organizer: 第65厘1日本栄養の食糧学会大会
  • Place of Presentation: お茶の水女子大学(東京)
  • Year and Date: 2011-05-14
• [Presentation] Mass spectrometry-based metabolic profiling of transient middle cerebral artery occlusion model (2010)
  • Author(s): Miura D, Fujimura Y, Yamato M, Hyodo F, Irie M, Tachibana H, and Wariishi H
  • Organizer: PACIFICHEM
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2010-12-19
• [Presentation] Visualization of spatiotemporal metabolic behaviors by in situ metabolomic imaging with MALDI-MS (2010)
  • Author(s): Miura D, Fujimura Y, Yamato M, Hyodo F, Tachibana H, and Wariishi H.
  • Organizer: 58th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Salt Lake, Utah, USA
  • Year and Date: 2010-05-25
• [Remarks]
  • URL: http://redoxnavi.kyushuu_ac.jp/group2/