2011 Fiscal Year Final Research Report
Establishment of sperm cells in vitro from female mouse somatic cells
| Project/Area Number |
22658086
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
KANEDA Masahiro 独立行政法人農業・食品産業技術総合研究機構, 畜産草地研究所・家畜育種繁殖研究領域, 主任研究員 (80469840)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 発生工学 / 生殖細胞 |
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| Research Abstract |
Genomic imprinting is indispensable for the proper mammalian development and is established during gametogenesis. I focused on DNA methylation, which plays an essential role for the establishment of imprinting, to create sperm-like cells from female mouse somatic cells in vitro. Three different types of DNA methylation inhibitors were added to mouse embryonic fibroblast and DNA methylation status was analyzed. There was a decrease of DNA methylation levels in some imprinted genes, however, no inhibitors could induce hypo-methylation in all imprinted genes analyzed.
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