Development of analytical methods of carbon and nitrogen stable isotope ratio in nucleic acids

2011 Fiscal Year Final Research Report

• Project/Area Number: 22658107
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Boundary agriculture
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: NUNOURA Takuro 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (60359164)
• Co-Investigator(Kenkyū-buntansha): TAKANO Yoshinori 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (80399815)
• Project Period (FY): 2010 – 2011
• Keywords: 生物環境 / 安定同位体

Research Abstract

The objective of this project was to organize a novel stable isotope technology for nucleotide especially rRNA in order to develop a novel methodology in environmental omics. In this study, we optimized the online separation and detection for deoxyribonucleotide by using high performance liquid chromatography combined with electrospray ionization mass spectrometry(unpublished). However, we could not construct a new methods for purification of nucleotides to use stable isotopic analysis due to several technical difficulties.