2011 Fiscal Year Final Research Report
Development of a new analytical system for bile acid metabolome
| Project/Area Number |
22659007
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Miki 東北大学, 病院, 准教授 (10196488)
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| Co-Investigator(Renkei-kenkyūsha) |
MAEKAWA Masamitsu 東北大学, 病院, 助手 (70572882)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 分析化学 / メタボロミクス / LC/MS / 胆汁酸 / 低エネルギーCID / 多次元マップ化 / 3β-HSD欠損症 / Niemann-Pick病typeC |
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| Research Abstract |
We set up an HPLC separation method for various bile acid conjugates, and characterized each fragmentation of bile acid conjugates by the investigation of product ion patterns on low-energy collision-induced dissociation. Based on their data, we produced focusing methods for bile acid metabolome by conjugated forms using precursor ion scan and neutral loss scan analysis. In addition, we analyzed urine from patients producing metabolic abnormalities of cholesterol, and found to analyze disease-specific bile acid metabolites.
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[Journal Article] A rapid and sensitive LC/ESI-MS/MS method for quantitative analysis of docetaxel in human plasma and its application to a pharmacokinetic study2012
Author(s)
Hiroaki Yamaguchi, Asuka Fujiwara, Hajime Ito, Nobuaki Tanaka, Ayako Furugen, Kazuaki Miyamori, Natsuko Takahashi, Jiro Ogura, Masaki Kobayashi, Takehiro Yamada, Nariyasu Mano, and Ken Iseki
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Journal Title
J. Chromatogr. B.
Volume: 893-894
Pages: 157-161
Peer Reviewed
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[Journal Article] Biliary bile acids in birds of the Cotingidae family : Taurine-conjugated (24R, 25R)-3α, 7α, 24-trihydroxy-5β-cholestan-27-oic acid and two epimers (25R and 25S)of 3α, 7α-dihydroxy-5β-cholestan-27-oic acid2011
Author(s)
Lee R. Hagey, Takashi Iida, Shoujiro Ogawa, Yuuki Adachi, Mizuho Une, Kumiko Mushiake, Masamitsu Maekawa, Miki Shimada, Nariyasu Mano, and Alan F. Hofmann
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Journal Title
Steroids
Volume: 76
Pages: 1126-1135
Peer Reviewed
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[Journal Article] Quantitation of five prostanoids in both cultured cells and media by liquid chromatography/electrospray ionization tandem mass spectrometry2011
Author(s)
Ayako Furugen, Hiroaki Yamaguchi, Nobuaki Tanaka, Hajime Ito, Kazuaki Miyamori, Asuka Fujikawa, Natsuko Takahashi, Jiro Ogura, Masaki Kobayashi, Takehiro Yamada, Nariyasu Mano, and Ken Iseki
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Journal Title
J. Chromatogr. B
Volume: 879
Pages: 3378-3385
Peer Reviewed
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[Journal Article] Potential corticoid metabolites: Chemical synthesis of 3-and 21-monosulfates and their double-conjugates of tetrahydrocorticosteroids in the 5α-and 5β-series2010
Author(s)
Rika Okihara, Kuniko Mitamura, Maki Hasegawa, Megumi Mori, Akina Muto, Genta Kakiyama, Shoujiro Ogawa, Takashi Iida, Miki Shimada, Nariyasu Mano, and Shigeo Ikegawa
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Journal Title
Chem. Pharm. Bull.
Volume: 58
Pages: 344-353
Peer Reviewed
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[Journal Article] Major biliary bile acids of the Medaka (Oryzias latipes): 25R-and 25S-epimers of 3α, 7α, 12α-trihydroxy-5β-cholestanoic acid2010
Author(s)
Lee R. Hagey, Takashi Iida, Hideyuki Tamegai, Shoujiro Ogawa, Mizuho Une, Kiyoshi Asahina, Kumiko Mushiake, Takaaki Goto, Nariyasu Mano, Junichi Goto, Matthew D. Krasowski, and Alan F. Hofmann
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Journal Title
Zoo. Sci.
Volume: 27
Pages: 565-573
Peer Reviewed
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[Journal Article] Chemical synthesis of (22E)-3α, 6α, 7α, 12α-tetrahydroxy-5β-chol-22-en-24-oic acid and its N-acylamidated conjugates with glycine or taurine : Precursors of the [22, 23-3H] labelled tracers2010
Author(s)
Shoujiro Ogawa, Yuuki Adachi, Genta Kakiyama, Miki Shimada, Nariyasu Mano, Junichi Goto, and Takashi Iida
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Journal Title
Chem. Pharm. Bull
Volume: 58
Pages: 1103-1106
Peer Reviewed
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[Journal Article] Practice and awareness of physicians regarding casual-clinic blood pressure measurement in Japan2010
Author(s)
Mitsuru Kobayashi, Taku Obara, Takayoshi Ohkubo, Hidefumi Fukunaga, Michihiro Satoh, Hirohito Metoki, Kei Asayama, Ryusuke Inoue, Masahiro Kikuya, Nariyasu Mano, Masaaki Miyakawa, and Yutaka Imai
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Journal Title
Hypertens. Res
Volume: 33
Pages: 960-964
Peer Reviewed
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[Journal Article] DCK is frequently inactivated in acquired gemcitabine-resistant human cancer cells
Author(s)
Yuriko Saiki, Yuki Yoshino, Hiroko Fujimura, Tatsuya Manabe, Yuki Kudo, Miki Shimada, Nariyasu Mano, Tomohiro Nakano, Yoonha Lee, Shinjiro Shimizu, Shinya Oba, Sho Fujiwara, Hideyuki Shimizu, Na Chen, Zhaleh K Nezhad, Guo Jin, Shinichi Fukushige, Makoto S
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Journal Title
Biochem. Biophys. Res. Commun
Volume: (in press)
Peer Reviewed
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[Presentation] Determination of multi-conjugated bile acids in the urine of patients with Niemann-Pick disease, type C by liquid chromatography/electrospray ionization tandem mass spectrometry2010
Author(s)
Masamitsu Maekawa, Yasushi Misawa, Ayako Sotoura, Osamu Sakamoto, Toshihiro Ohura, Daiki Abukawa, Takehiko Inoue, Kousaku Ohno, Akina Muto, Hajime Takei, Hiroshi Nittono, Shoujiro Ogawa, Genta Kakiyama, Takashi Iida, Alan F. Hofmann, Junichi Goto, Miki Sh
Organizer
XXI International Bile Acid Meeting ; Bile Acids as Metabolic Integrators and Therapeutics
Place of Presentation
Freiburg, Germany
Year and Date
20101007-1008
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