2011 Fiscal Year Final Research Report
Establishment of the methodology for target identification of bioactive natural products based on the chemical genomics
| Project/Area Number |
22659022
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Drug development chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
ARAI Masayoshi 大阪大学, 大学院・薬学研究科, 准教授 (80311231)
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| Project Period (FY) |
2010 – 2011
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| Keywords | ケミカルジェノミクス / ケミカルバイオロジー / 生物活性物質 / 標的分子 |
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| Research Abstract |
With the transformants of HeLa cells with cDNA library or the next-generation sequencer, we tried establishment of the general methodology to clarify the target molecules of the bioactive natural products that acts on the eukaryotes such as an animal cells. Using topoisomerase I inhibitor of camptothecin as a model compound, we succeeded in the establishment of the methods, which selects a camptothecin-resistant HeLa cell from the transformants of HeLa cells that over-expressed cDNA randomly, and analyzes the cDNA introduced from the camptothecin-resistant HeLa cell. We also succeeded in the preparation of spontaneous resistant HeLa cells against camptothecin for exhaustively analyzing genetic mutation using a next-generation sequencer.
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