Development of the attogram protein detection system using cultured cell lines and chimeric molecules

2012 Fiscal Year Final Research Report

• Project/Area Number: 22659027
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Environmental pharmacy
• Research Institution: National Institute of Health Sciences
• Principal Investigator: NAKAMURA Ryosuke 国立医薬品食品衛生研究所, 代謝生化学部, 主任研究官 (50333357)
• Research Collaborator: NAKAMURA Rika (40643333)
• Project Period (FY): 2010 – 2012
• Keywords: 食品衛生学

Research Abstract

Using a rat mast cell line that has been stably integrated with human high-affinity IgE receptor cDNAs and also with NF-AT-driven luciferase reporter gene, attogram-level of hydrolyzed wheat protein-derived allergenic protein was detected.

Research Products

• [Journal Article] Evaluation of allergenicity of acidhydrolyzed wheat protein using an in vitro elicitation test (2013)
  • Author(s): Nakamura, R., Nakamura, R., Adachi, R., Itagaki, Y., Fukutomi, Y., Teshima, R
  • Journal Title: nternational Archives of Allergy and Immunology Volume: 160 Pages: 259-264
  • DOI: DOI: 10.1159/000341671
  • Peer Reviewed
• [Journal Article] 特集「アレルギー疾患における特異抗体の意義」. II.アレルゲン特異IgE 抗体の新しい測定方法「4.EXiLE法」 (2013)
  • Author(s): 中村亮介、手島玲子
  • Journal Title: アレルギー・免疫 Volume: 20 Pages: 63-73
• [Journal Article] Evaluation of the luciferase assay-based in vitro elicitation test for serum IgE (2012)
  • Author(s): Nakamura, R., Ishiwatari, A., Higuchi, M., Uchida, Y., Nakamura, R., Kawakami, H., Urisu, A., Teshima, R
  • Journal Title: Allergology international Volume: 61 Pages: 431-437
  • DOI: DOI: 10.2332/allergolint.11-OA-0407
  • Peer Reviewed
• [Journal Article] 2D-DIGE analysis of rice proteins from different cultivars (2010)
  • Author(s): Teshima, R., Nakamura, R., Satoh, R., Nakamura, R
  • Journal Title: Regulatory Toxicology Pharmacology Volume: 58 Pages: S30-35
  • DOI: DOI: 10.1016/j.yrtph.2010.05.010
  • Peer Reviewed
• [Journal Article] A convenient and sensitive allergy test: IgE crosslinking-induced luciferase expression in cultured mast cells (2010)
  • Author(s): Nakamura, R., Uchida, Y., Higuchi, M., Nakamura, R., Tsuge, I., Urisu, A., Teshima, R
  • Journal Title: Allergy Volume: 65(10) Pages: 1266- 1273
  • DOI: DOI: 10.1111/j.1398-9995.2010.02363.x
  • Peer Reviewed
• [Journal Article] Effects of propolis from different areas on mast cell degranulation and identification of the effective components in propolis (2010)
  • Author(s): Nakamura, R., Nakamura R., Watanabe, K., Oka, K., Ohta, S., Mishima, S., Teshima, R
  • Journal Title: Internationial Immunopharmacology Volume: 10 Pages: 1107-1112
  • DOI: DOI: 10.1016/j.intimp.2010.06.013
  • Peer Reviewed
• [Presentation] EXiLE 法における抗原の固相化の影響 (2013)
  • Author(s): 中村亮介, 中村里香, 手島玲子
  • Organizer: 日本薬学会第133 年会
  • Year and Date: 20130300
• [Presentation] EXiLE 法による加水分解小麦のアレルゲン性における分子サイズの影響の解析 (2012)
  • Author(s): 中村亮介, 中村里香, 酒井信夫, 安達玲子, 福冨友馬, 手島玲子
  • Organizer: 第85回日本生化学会
  • Year and Date: 20121200
• [Presentation] In vitro provocation study (2012)
  • Author(s): Nakamura, R
  • Organizer: The 51st meeting of Society of Toxicology
  • Year and Date: 20120300
• [Presentation] EXiLE: a novel reporter assay-based allergy test (2011)
  • Author(s): Nakamura, R., Ishiwatari, A., Nakamura, R., Kawakami, H., Tsuge,I., Urisu, A., Teshima, R
  • Organizer: Joint Congress of APAPARI 2011 & 48th JSPACI
  • Year and Date: 20111000
• [Presentation] 培養細胞のルシフェラーゼ発現を指標とするアレルギー試験法 (2011)
  • Author(s): 中村亮介, 石渡亜耶乃, 川上浩, 手島玲子
  • Organizer: 第4回食品薬学シンポジウム
  • Year and Date: 20111000
• [Presentation] 子どもと免疫: 小児食物アレルギー試験の観点から (2011)
  • Author(s): 中村亮介
  • Organizer: 第18回日本免疫毒性学会学術大会
  • Year and Date: 20110900
• [Presentation] 新しいアレルギー試験法EXiLE 法による加熱卵白アレルゲンのIgE 応答性の解析 (2011)
  • Author(s): 中村亮介, 石渡亜耶乃, 樋口雅一, 中村里香, 川上浩, 手島玲子
  • Organizer: 日本薬学会第131 年会
  • Year and Date: 20110300
• [Presentation] 培養細胞を用いた新規アレルギー試験法の開発. (2010)
  • Author(s): 中村亮介, 樋口雅一, 内田好海, 中村里香, 柘植郁哉, 宇理須厚雄, 手島玲子
  • Organizer: 第60回日本アレルギー学会秋季学術大会
  • Year and Date: 20101100
• [Presentation] EXiLE 法を用いたアレルゲン交差反応性の解析 (2010)
  • Author(s): 中村亮介, 樋口雅一, 中村里香, 近藤康人, 宇理須厚雄, 手島玲子
  • Organizer: 第17回日本免疫毒性学会学術大会
  • Year and Date: 20100900
• [Presentation] IgE crosslinking- induced luciferase expression (EXiLE) as a sensitive indicator for serum-based allergy test (2010)
  • Author(s): Nakamura, R., Uchida, Y., Higuchi, M., Nakamura, R., Teshima, R
  • Organizer: EAACI2010
  • Year and Date: 20100600