2011 Fiscal Year Final Research Report
A strategy for the identification of HBV receptors with HBV pseudotype
| Project/Area Number |
22659091
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Osaka University |
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Principal Investigator |
UEDA Keiji 大阪大学, 大学院・医学系研究科, 教授 (00221797)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANO Kazushi 大阪大学, 大学院・医学系研究科, 助教 (00521725)
OHSAKI Eriko 大阪大学, 大学院・医学系研究科, 助教 (50447801)
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| Project Period (FY) |
2010 – 2011
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| Keywords | B型肝炎ウイルス / pseudotype / 感染受容体 |
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| Research Abstract |
It is near a half century since hepatitis B virus(HBV) was identified. An iceberg of HBV receptor has not been elucidated, though there are some reports on infection systems and on the receptor molecules. Thus, we still have not reached finding a real HBV receptor and there have been no useful and convenient infection system in vitro and in vivo for HBV, which makes it impossible for us to understand a precise HBV life cycle and HBV involved related diseases. Here, we designed and tried to generate an HBV pseudotype, which had a viral particle containing a retrovirus capsid and a genome inside surrounded by HBV membrane proteins. We proved successful generation of this pseudotype by immunoprecipitation with anti-HBVs antibodies and by CsCl density gradient ultracentrifugation, followed by RT-PCR targeting a retroviral gene, a GFP gene in this case, respectively. Though our established system is constructed on growth dependent integration of retroviral genomes and thus was very hard to observe its infection in a primary human hepatocytes culture system, successful generation of the HBV pseudotype will make it possible for us to perform a biological assay to clone an HBV receptor based on infectivity and will facilitate its separation and identification.
We have not identified an HBV receptor candidate yet. It has been suggested, however, that there could be an attachment factor in ordinary cultured cell lines derived from hepatocellular carcinomas.
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[Journal Article] Kaposi' s sarcoma-associated Virus governs gene expression profiles toward B cell transformation2012
Author(s)
Ueda, K., Ito, E., Karayama, M., Ohsaki, E., Nakano, K., and Watanabe, S
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Journal Title
In-Tech" Herpesviruses", Magel, D. G. and Tyring, S., ed.
Volume: (in press)
Pages: 978-953-51-0186-4
Peer Reviewed
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[Journal Article] Overexpression of HGF attenuates the degeneration of Purkinje cells and Bergmann glia in a knockin mouse model of spinocerebellar ataxia type 7.2012
Author(s)
Noma, S., Ohya-Shimada, W., Kanai, M., U ed a, K., Nakamura, T., Funakoshi, H.
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Journal Title
Neuroscience Res
Volume: (in press)
DOI
Peer Reviewed
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[Journal Article] Novel Monoclonal Antibodies for Identification of Multicentric Castleman' s Disease ; Kaposi' s Sarcoma-Associated Herpesvirus-Encoded vMIP-I and vMIP-II.2012
Author(s)
Nakano, K., Katano, H., Tadagaki, K., Sato, Y., Ohsaki, E., Mori, Y., Yamanishi, K., and Ueda, K.
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Journal Title
Virology
Volume: 425
Pages: 95-102
DOI
Peer Reviewed
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[Journal Article] KSHV-infected PEL cell lines exhibit a distinct gene expression profile2010
Author(s)
Ueda, K., Ito, E., Karayama, M., Ohsaki, E., Nakano, K., Watanabe, S.
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Journal Title
Biochem. Biophsys. Res. Comm.
Volume: 394
Pages: 482-487
Peer Reviewed
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