2012 Fiscal Year Final Research Report
Establishment of an interferon transfer factor prevention cell strainuseful on virus hunting
| Project/Area Number |
22659092
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HAYASHI Hideki 長崎大学, 医歯薬学総合研究科, 准教授 (10218589)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ウイルス / インターフェロン / IRF / iPS細胞 / ワクチン |
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| Research Abstract |
The goal of our project is to establish interferon (IFN) signaling-deficient cell lines that are highly susceptible to viral infection, proliferation and survival. In particular, we target those viruses which are proven difficult to isolate by established protocols. We transformed 293T cells with plasmids that have negative effects on various IFN-signal transduction molecules, and subsequently confirmed by monitoring IFN-stimulated genes. Our final goal is to establish iPS cells deficient in IFN signaling. As iPS cells are pluripotent, we hypothesized that once established, we can manipulate these iPS cells to differentiate into different tissue types. This will become a very powerful tool for the isolation of tissue-specific viruses. Currently, we focus our effort in generating iPS cells deficient in IFN signaling. We expect these cells will become available soon and hope to translate it for clinical use in the near future.
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[Journal Article] Kamiyama H, Kakoki K, Shigematsu S, Izumida M, Yashima Y, Tanaka Y, Hayashi H, Matsuyama T, Sato H, Yamamoto N, Sano T, Shidoji Y, Kubo Y2013
Author(s)
CXCR4-Tropic, But Not CCR5-Tropic, Human Immunodeficiency Virus Infection Is Inhibited by the Lipid Raft-Associated Factors, Acyclic Retinoid Analogs, and Cholera Toxin B Subunit
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Journal Title
AIDS Res Hum Retroviruses
Volume: 29(2)
Pages: 279-88
Peer Reviewed
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[Journal Article] CD4-independent humanimmunodeficiency virus infection involves participation of endocytosis and cathepsin B2011
Author(s)
Yoshii H, Kamiyama H, Goto K, Oishi K, Katunuma N, Tanaka Y, Hayashi H, Matsuyama T, Sato H, Yamamoto N, Kubo Y
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Journal Title
PLoS One
Volume: 6(4)
Pages: e19352
Peer Reviewed
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[Journal Article] Shared and distinct functions of the transcription factors IRF4 and IRF8 in myeloid cell development2011
Author(s)
Yamamoto M, Kato T, Hotta C, Nishiyama A,KurotakiD,YoshinariM,TakamiM,IchinoM,NakazawaM,MatsuyamaT,KamijoR,KitagawaS,OzatoK,TamuraT
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Journal Title
PLoS One
Volume: 6(10)
Pages: e25812
Peer Reviewed
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[Journal Article] Characterization of dsRNA-induced pancreatitis model reveals the regulatory role of IRF-2 in trypsinogen5 gene transcription2011
Author(s)
Hayashi H, Kohno T, Yasui K, Murota H, Kimura T, Duncan GS, Nakashima T, Yamamoto K, Katayama I, Ma Y, Chua KJ, Suematsu T, Shimokawa I, Akira S, Kubo Y, Mak TW, Matsuyama T
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Journal Title
Proc Natl Acad Sci U S A
Volume: 108(46)
Pages: 18766-71
Peer Reviewed
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[Journal Article] Interferon regulatory factor-4 activates IL-2 and IL-4 promoters in cooperation with c-Rel2011
Author(s)
Shindo H, Yasui K, Yamamoto K, Honma K, Yui K, Kohno T, Ma Y, Chua KJ, Kubo Y, Aihara H, Ito T, Nagayasu T, Matsuyama T, Hayashi H
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Journal Title
Cytokine
Volume: 56(3)
Pages: 564-72
DOI
Peer Reviewed
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[Journal Article] Aberrant expression of interferon regulatory factor 3 in human lung cancer.2010
Author(s)
Tokunaga T, Naruke Y, Shigematsu S, Kohno T, Yasui K, Ma Y, Chua KJ, Katayama I, Nakamura T, Hishikawa Y, Koji T, Yatabe Y, Nagayasu T, Fujita T,Matsuyama T, Hayashi H
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Journal Title
Biochem Biophys Res Commun
Volume: 397(2)
Pages: 202-7
Peer Reviewed
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[Journal Article] The Jmjd3-Irf4 axis regulates M2 macrophage polarization and host responses against helminth infection2010
Author(s)
Satoh T, Takeuchi O, Vandenbon A, Yasuda K, Tanaka Y, Kumagai Y, Miyake T,Matsushita K, Okazaki T, Saitoh T, Honma K, Matsuyama T, Yui K, Tsujimura T,Standley DM, Nakanishi K, Nakai K, Akira S.
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Journal Title
Nat Immunol
Volume: 11(10)
Pages: 936-44
Peer Reviewed
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