Trichloroethylene-induced hypersensitivity may be caused by its active metabolite-protein adducts

2011 Fiscal Year Final Research Report

• Project/Area Number: 22659123
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Hygiene
• Research Institution: Nagoya University
• Principal Investigator: NASU Tamie 名古屋大学, 大学院・医学系研究科, 教授 (10020794)
• Project Period (FY): 2010 – 2011
• Keywords: インターフェロンγ / 肝臓 / γGTP / 好酸球 / CYP2E1 / トリクロロエチレン / トリクロロエタノール / トリクロロ酢酸 / 皮膚

Research Abstract

We investigated skin and liver damages associated with trichloroethylene-, trichloroethanol-and trichloroacetic acid-induved hypersensitive skin reactions in the hartley guinea pig maximization test. The sensitization rates of trichloroethylene-induced dermal allergy were 70% in male guinea pigs, and 90% in female ones. Those of trichloroethanol were 20% in male guinea and 50% in female ones, while only a minor sensitization was seen after trichloroacetic acid treatment. In relation to the sensitization, eosinocytes were seen in the skin, and infiltration of lymphocytes was seen around focal necrosis in the liver. These changes were seen most prominent in trichloroethylene-exposed animals, followed by trichloroethanol. Trichloroethylene exposure also increased plasmaγGTP and interferonγlevels, while trichloroethanol and trichloroacetic acid did not.

Research Products

• [Journal Article] Differential Response to Trichloroethylene-Induced Hepatosteatosis in Wild-Type and PPARα-Humanized Mice (2010)
  • Author(s): Ramdhan DH, Kamijima M, Wang D, Ito Y, Naito H, Yanagiba Y, Hayashi Y, Tanaka N, Aoyama T, Gonzalez FJ, Nakajima T
  • Journal Title: Environ Health Perspect Volume: 118 Pages: 1557-1563
  • Peer Reviewed
• [Presentation] モルモットを用いたトリクロロエチレンによる皮膚・肝障害のメカニズム解析 (2012)
  • Author(s): 内藤久雄、日比野優衣、斉藤優、王棟、趙娜、宋向栄、王海蘭、上島通浩、那須民江
  • Organizer: 第85回日本産業衛生学会総会
  • Place of Presentation: 名古屋名古屋国際会議場
  • Year and Date: 2012-05-31
• [Presentation] リクロロエチレン曝露による免疫性肝障害に関する研究 (2012)
  • Author(s): 日比野優衣、内藤久雄、斉藤優、王棟、趙娜、宋向栄、王海蘭、上島通浩、那須民江
  • Organizer: 第82回日本衛生学会学術総会
  • Place of Presentation: 京都、京都大学
  • Year and Date: 2012-03-25
• [Presentation] DIFFERENTIAL RESPONSE TOTRICHLOROETHYLENE-INDUCEDHEPATOSTEATOSIS IN WILD-TYPE AND PPARA-HUMANIZED MICE (2010)
  • Author(s): Ramdhan DH, Kamijima M, Wang D, Ito Y, Yanagiba Y, Hayashi Y, Naito H, Gonzalez FJ, Nakajima T
  • Organizer: XII International Congress of Toxicology
  • Place of Presentation: Barcelona-Spain Palau de Congressosde Barcelona
  • Year and Date: 20100719-23
• [Presentation] トリクロロエチレン誘発性肝障害におけるPPARα活性の種差による影響について (2010)
  • Author(s): Ramdhan DH,上島通浩,王棟,伊藤由起,柳場由絵,林由美,内藤久雄, Gonzalez FJ,那須民江
  • Organizer: 第83回日本産業衛生学会総会
  • Place of Presentation: 福井、福井国際交流会館
  • Year and Date: 2010-05-28