ヒトの肝臓を主座とする遺伝性疾患を有するマウスモデルの創生

2011 Fiscal Year Annual Research Report

• Project/Area Number: 22659147
• Research Institution: Hiroshima University
• Principal Investigator: 茶山 一彰 広島大学, 大学院・医歯薬学総合研究科, 教授 (00211376)
• Keywords: 肝臓学 / ノックアウトマウス / トランスジェニックマウス / 不死化肝細胞

Research Abstract

ヒトの遺伝性疾患は、ノックアウトマウスなどを使用して研究されていることが多い。しかし、マウスとヒトの病態は必ずしも同一ではなく、また、マウスで耐性致死であるために良好なモデルが作製できない場合も多い。また、ノックアウトマウスは作製に多大な時間と人手を要するため、わずかの違いを有するマウスを作製するのにも再度全く同じ手間を要する。本研究では、培養ヒト肝細胞に遺伝子導入することにより、ヒトの疾患と同様の状態の肝細胞を作製し、この細胞をuPA-scid mouseに移植することにより、遺伝病状態のヒト肝臓を有するマウスを作製することを目的として研究を行った。このマウスに、遺伝病の遺伝子を導入することは技術的に困難であったが、ヒト肝細胞キメラマウスにB型肝炎ウイルスを感染させ、さらにヒトリンパ球を投与することにより、ヒト肝細胞キメラマウスに劇症肝炎に類似した肝細胞の広範な壊死をもたらすモデルを作製することができた。この肝炎モデルではこれまでに想定されていたcytotoxicTlymphocyteではなく、FAS抗原を提示している肝細胞に対するNatural killer cellと樹状細胞が重要な役割を果たしていることが明らかになった。さらに、アルファインターフェロン、ガンマインターフェロンはこのモデルでは重要な役割を果たしておらず、他のサイトカインが肝炎の発症に必要であることが想定された。B型劇症肝炎は遺伝病ではないが、劇症肝炎も治療がきわめて困難な疾患であり、貴重なモデルを作製することができた。

Research Products

• [Journal Article] Toward the establishment of a prediction system for the personalized treatment of chronic hepatitis C (2012)
  • Author(s): Ochi H, Hayes CN, Abe H, Hayashida Y, Uchiyama T, Kamatani N, Nakamura Y, Chayama K.
  • Journal Title: J Infect Dis Volume: 205 Pages: 204-210
  • DOI: doi:10.1093/infdis/jir726
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• [Journal Article] Impact of pegylated interferon therapy on outcomes of patients with hepatitis C virus-related hepatocellular carcinoma after curative hepatic resection (2012)
  • Author(s): Tanimoto Y, Tashiro H, Aikata H, Amano H, Oshita A, Kobayashi T, Kuroda S, Tazawa H, Takahashi S, Itamoto T, Chayama K, Ohdan H
  • Journal Title: Ann Surg Oncol Volume: 19 Pages: 418-425
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• [Journal Article] Severe necroinflammatory reaction caused by natural killer by Fas/FasL interaction and dendritic cells in human hepatocyte chimeric mouse (2012)
  • Author(s): Okazaki A, Hiraga N, Imamura M, Nelson Hayes C, Tsuge M, Takahashi S, Aikata H, Abe H, Miki D, Ochi H, Tateno C, Yoshizato K, Ohdan H, Chayama K
  • Journal Title: Hepatology Volume: (in press)
  • DOI: 10.1002/hep.25651
  • Peer Reviewed
• [Journal Article] IL28B polymorphism is associated with fatty change in the liver of chronic hepatitis C patients (2012)
  • Author(s): Ohnishi M, Tsuge M, Kohno T, Zhang Y, Abe H, Hyogo H, Kimura Y, Miki D, Hiraga N, Imamura M, Takahashi S, Ochi H, Hayes CN, Tanaka S, Arihiro K, Chayama K
  • Journal Title: J Gastroenterol Volume: (in press)
  • DOI: 10.1007/s00535-012-0550-y
  • Peer Reviewed
• [Journal Article] Serum PAI-1 is a novel predictor for response to pegylated interferon-alpha-2b plus ribavirin therapy in chronic hepatitis C virus infection (2012)
  • Author(s): Miki D, Ohishi W, Ochi H, Hayes CN, Abe H, Tsuge M, Imamura M, Kamatani N, Nakamura Y, Chayama K
  • Journal Title: J Viral Hepat Volume: 19 Pages: e126-133
  • DOI: 10.1111/j.1365-2893.2011.01516.x
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• [Journal Article] Hepatocellular carcinoma : towards personalized medicine (2012)
  • Author(s): Miki D, Ochi H, Nelson Hayes C, Aikata H, Chayama K
  • Journal Title: Cancer Sci Volume: (in press)
  • DOI: 10.1111/j.1349-7006.2012.02242.x
  • Peer Reviewed
• [Journal Article] Combination of hepatitis B viral antigens and DNA for prediction of relapse after discontinuation of nucleos(t)ide analogs in patients with chronic hepatitis B (2012)
  • Author(s): Matsumoto A, Tanaka E, Suzuki Y, Kobayashi M, Tanaka Y, Shinkai N, Hige S, Yatsuhashi H, Nagaoka S, Chayama K, Tsuge M, Yokosuka O, Imazeki F, Nishiguchi S, Saito M, Fujiwara K, Torii N, Hiramatsu N, Karino Y, Kumada H
  • Journal Title: Hepatol Res Volume: 42 Pages: 139-149
  • DOI: 10.1111/j.1872-034X.2011.00910.x
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• [Journal Article] Efficacy and safety of telaprevir, a new protease inhibitor, for difficult-to-treat patients with genotype 1 chronic hepatitis C (2012)
  • Author(s): Hayashi N, Okanoue T, Tsubouchi H, Toyota J, Chayama K, Kumada H
  • Journal Title: J Viral Hepat Volume: 19 Pages: e134-142
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• [Journal Article] Prevalence and associated metabolic factors of nonalcoholic fatty liver disease in the general population from 2009 to 2010 in Japan : a multicenter large retrospective study (2012)
  • Author(s): Eguchi Y, Hyogo H, Ono M, Mizuta T, Ono N, Fujimoto K, Chayama K, Saibara T, Jsg N
  • Journal Title: J Gastroenterol Volume: (in press)
  • DOI: 10.1007/s00535-012-0533-z
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• [Journal Article] Validation of the FIB4 index in a Japanese nonalcoholic fatty liver disease population (2012)
  • Author(s): Sumida Y, Yoneda M, Hyogo H, Itoh Y, Ono M, Fujii H, Eguchi Y, Suzuki Y, Aoki N, Kanemasa K, Fujita K, Chayama K, Saibara T, Kawada N, Fujimoto K, Kohgo Y, Yoshikawa T, Okanoue T, Jsg-Nafld JS
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  • DOI: 10.1186/1471-230X-12-2
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• [Journal Article] Identification of the Niemann-Piok C1-like 1 cholesterol absorption receptor as a new hepatitis C virus entry factor (2012)
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  • DOI: 10.1038/nm.2581
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• [Journal Article] Telaprevir with peginterferon and ribavirin for treatment-naive patients chronically infected with HCV of genotype 1 in Japan (2012)
  • Author(s): Kumada H, Toyota J, Okanoue T, Chayama K, Tsubouchi H, Hayashi N
  • Journal Title: J Hepatol Volume: 56 Pages: 78-84
  • DOI: 10.1016/j.jhep.2011.07.016
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• [Journal Article] Atorvastatin improves disease activity of nonalcoholic steatohepatitis partly through its tumour necrosis factor-alpha-lowering property (2012)
  • Author(s): Hyogo H, Yamagishi SI, Maeda S, Kimura Y, Ishitobi T, Chayama K
  • Journal Title: Dig Liver Dis Volume: (in press)
  • DOI: 10.1016/j.dld.2011.12.013
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• [Journal Article] Dual therapy with the nonstructural Protein 5A Inhibitor, BMS-790052, and the Nonstructural Protein 3 Protease Inhibitor, BMS-650032, in Hepatitis C Virus Genotype 1b-Infected Null Responders (2012)
  • Author(s): Chayama K, Takahashi S, Toyota J, Karino Y, Ikeda K, Ishikawa H, Watanabe H, McPhee F, Hughes E, Kumada H
  • Journal Title: Hepatology Volume: 55 Pages: 742-8
  • DOI: 10.1002/hep.24724
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• [Journal Article] Amino Acid Substitution in HCV Core/NS5A Region and Genetic Variation Near IL28B Gene Affect Treatment Efficacy to Interferon plus Ribavirin Combination Therapy (2012)
  • Author(s): Akuta N, Suzuki F, Hirakawa M, Kawamura Y, Sezaki H, Suzuki Y, Hosaka T, Kobayashi M, Kobayashi M, Saitoh S, Arase Y, Ikeda K, Chayama K, Nakamura Y, Kumada H
  • Journal Title: Intervirology Volume: 55 Pages: 231-41
  • DOI: 10.1159/000328327
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• [Journal Article] Rapid emergence of telaprevir resistant hepatitis C virus strain from wildtype clone in vivo (2011)
  • Author(s): Hiraga N, Imamura M, Abe H, Hayes CN, Kono T, Onishi M, Tsuge M, Takahashi S, Ochi H, Iwao E, Kamiya N, Yamada I, Tateno C, Yoshizato K, Matsui H, Kanai A, Inaba T, Tanaka S, Chayama K.
  • Journal Title: Hepatology 2011 Volume: 54 Pages: 781-788
  • DOI: doi:10.1002/hep.24460
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• [Journal Article] IL28 variation affects expression of interferon stimulated genes and peg-interferon and ribavirin therapy (2011)
  • Author(s): Abe H, Hayes CN, Ochi H, Maekawa T, Tsuge M, Miki D, Mitsui F, Hiraga N, Imamura M, Takahashi S, Kubo M, Nakamura Y, Chayama K.
  • Journal Title: Hepatol 2011 Volume: 54 Pages: 1094-1101
  • DOI: doi:10.1016/j.jhep.2010.09.019
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• [Journal Article] A randomized phase II trial of intra-arterial chemotherapy using SM-11355 (Miriplatin) for hepatocellular carcinoma (2011)
  • Author(s): Okusaka T, Kasugai H, Ishii H, Kudo M, Sata M, Tanaka K, Shioyama Y, Chayama K, Kumada H, Yoshikawa M, Seki T, Saito H, Hayashi N, Shiratori K, Okita K, Sakaida I, Honda M, Kusumoto Y, Tsutsumi T, Sakata K
  • Journal Title: Invest New Drugs Volume: (Published online : 21 Dec.)(Epub)
  • DOI: 10.1007/s10637-011-9776-4
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• [Journal Article] Treatment of chronic hepatitis B with nucleos(t)ide analogues (2011)
  • Author(s): Ohishi W, Chayama K
  • Journal Title: Hepatol Res Volume: 42 Pages: 219-25
  • DOI: 10.1111/j.1872-034X.2011.00921.x
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• [Journal Article] Recent trend of clinical features in patients with hepatocellular carcinoma (2011)
  • Author(s): Nagaoki Y, Hyogo H, Aikata H, Tanaka M, Naeshiro N, Nakahara T, Honda Y, Miyaki D, Kawaoka T, Takaki S, Hiramatsu A, Waki K, Imamura M, Kawakami Y, Takahashi S, Chayama K
  • Journal Title: Hepatol Res Volume: (Epub 13 Dec.)(Epub)
  • DOI: 10.1111/j.1872-034X.2011.00929.x
  • Peer Reviewed
• [Journal Article] Platelet count for predicting fibrosis in nonalcoholic fatty liver disease (2011)
  • Author(s): Yoneda M, Fujii H, Sumida Y, Hyogo H, Itoh Y, Ono M, Eguchi Y, Suzuki Y, Aoki N, Kanemasa K, Imajo K, Chayama K, Saibara T, Kawada N, Fujimoto K, Kohgo Y, Yoshikawa T, Okanoue T, Japan Study Group of Nonalcoholic Fatty Liver D
  • Journal Title: J Gastroenterol Volume: 46 Pages: 1300-1306
  • DOI: 10.1007/s00535-011-0436-4
  • Peer Reviewed
• [Journal Article] Clinical outcome of esophageal varices after hepatic arterial infusion chemotherapy for advanced hepatocellular carcinoma with major portal vein tumor thrombus (2011)
  • Author(s): Kodama H, Aikata H, Murakami E, Miyaki D, Nagaoki Y, Hashimoto Y, Azakami T, Katamura Y, Kawaoka T, Takaki S, Hiramatsu A, Waki K, Imamura M, Kawakami Y, Takahashi S, Ishikawa M, Kakizawa H, Awai K, Kenjo M, Nagata Y, Chayama K
  • Journal Title: Hepatol Res Volume: 41 Pages: 1046-1056
  • DOI: 10.1111/j.1872-034X.2011.00857.x
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• [Journal Article] Eradication of hepatitis C virus genotype 1 after liver transplantation by interferon therapy before surgery : Report of three patients with analysis of interleukin-28 polymorphism, hepatitis C virus core region and in terferon-sensitivity determining region (2011)
  • Author(s): Kawaoka T, Aikata H, Miyaki D, Murakami E, Azakami T, Takaki S, Nagaoki Y, Hashimoto Y, Katamura Y, Hiramatsu A, Waki K, Hiraga N, Miki D, Tsuge M, Imamura M, Kawakami Y, Takahashi S, Ochi H, Tashiro H, Ohdan H, Chayama K
  • Journal Title: Hepatol Res Volume: 41 Pages: 1126-1131
  • DOI: 10.1111/j.1872-034X.2011.00853.x
  • Peer Reviewed
• [Journal Article] A huge metastatic liver tumor from leiomyosarcoma of the inferior venacava : report of a case (2011)
  • Author(s): Hashimoto M, Kobayashi T, Tashiro H, Amano H, Oshita A, Tanimoto Y, Kuroda S, Tazawa H, Aikata H, Chayama K, Fujii M, Arihiro K, Ohdan H
  • Journal Title: Surg Today Volume: (Epub 11 Nov.)(Epub)
  • DOI: 10.1007/s00595-011-0070-z
  • Peer Reviewed
• [Journal Article] Lymphocyte subset characterization associated with persistent hepatitis C virus infection and subsequent progression of liver fibrosis (2011)
  • Author(s): Yoshida K, Ohishi W, Nakashima E, Fujiwara S, Akahoshi M, Kasagi F, Chayama K, Hakoda M, Kyoizumi S, Nakachi K, Hayashi T, Kusunoki Y
  • Journal Title: Hum Immunol Volume: 72 Pages: 821-826
  • DOI: 10.1016/j.humimm.2011.05.029
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• [Journal Article] Prevention of hepatitis B virus reactivation in immunosuppressive therapy or chemotherapy (2011)
  • Author(s): Ohishi W, Chayama K
  • Journal Title: Clin Exp Nephrol Volume: 15 Pages: 634-640
  • DOI: 10.1007/s10157-011-0464-7
  • Peer Reviewed
• [Journal Article] A genome-wide association study of chronic hepatitis B identified novel risk locus in a Japanese population (2011)
  • Author(s): Mbarek H, Ochi H, Urabe Y, Kumar V, Kubo M, Hosono N, Takahashi A, Kamatani Y, Miki D, Abe H, Tsunoda T, Kamatani N, Chayama K, Nakamura Y, Matsuda K
  • Journal Title: Hum Mol Genet Volume: 20 Pages: 3884-3892
  • DOI: 10.1093/hmg/ddr301
  • Peer Reviewed
• [Journal Article] IL28B polymorphism may guide pegylated interferon plus ribavirin therapy even after curative treatment for hepatitis C virus-related hepatocellular carcinoma (2011)
  • Author(s): Kawaoka T, Aikata H, Takaki S, Hiramatsu A, Waki K, Hiraga N, Miki D, Tsuge M, Imamura M, Kawakami Y, Takahashi S, Ochi H, Tashiro H, Ohdan H, Chayama K
  • Journal Title: J Viral Hepat Volume: 18 Pages: e550-560
  • DOI: 10.1111/j.1365-2893.2011.01468.x
  • Peer Reviewed
• [Journal Article] Hepatitis C virus infection suppresses the interferon response in the liver of the human hepatocyte chimeric mouse (2011)
  • Author(s): Tsuge M, Fujimoto Y, Hiraga N, Zhang Y, Ohnishi M, Kohno T, Abe H, Miki D, Imamura M, Takahashi S, Ochi H, Hayes CN, Miya F, Tsunoda T, Chayama K
  • Journal Title: PLoS One Volume: 6 Pages: e23856
  • DOI: 10.1371/journal.pone.0023856
  • Peer Reviewed
• [Journal Article] Impact of viral amino acid substitutions and host interleukin-28b polymorphism on replication and susceptibility to interferon of hepatitis C virus (2011)
  • Author(s): Hiraga N, Abe H, Imamura M, Tsuge M, Takahashi S, Hayes CN, Ochi H, Tateno C, Yoshizato K, Nakamura Y, Kamatani N, Chayama K
  • Journal Title: Hepatology Volume: 54 Pages: 764-771
  • DOI: 10.1002/hep.24453
  • Peer Reviewed
• [Journal Article] Inverse association of IL28B genotype and liver mRNA expression of genes promoting or suppressing antiviral state (2011)
  • Author(s): Abe H, Hayes CN, Ochi H, Tsuge M, Miki D, Hiraga N, Imamura M, Takahashi S, Kubo M, Nakamura Y, Kamatani N, Chayama K
  • Journal Title: J Med Virol Volume: 83 Pages: 1597-1607
  • DOI: 10.1002/jmv.22158
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• [Journal Article] In vivo adaptation of hepatitis C virus in chimpanzees for efficient virus production and evasion of apoptosis (2011)
  • Author(s): Saeed M, Shiina M, Date T, Akazawa D, Watanabe N, Murayama A, Suzuki T, Watanabe H, Hiraga N, Imamura M, Chayama K, Choi Y, Krawczynski K, Liang TJ, Wakita T, Kato T
  • Journal Title: Hepatology Volume: 54 Pages: 425-433
  • DOI: 10.1002/hep.24399
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• [Journal Article] Variation in the DEPDC5 locus is associated with progression to hepatocellular carcinoma in chronic hepatitis C virus carriers (2011)
  • Author(s): Miki D, Ochi H, Hayes CN, Abe H, Yoshima T, Aikata H, Ikeda K, Kumada H, Toyota J, Morizono T, Tsunoda T, Kubo M, Nakamura Y, Kamatani N, Chayama K
  • Journal Title: Nat Genet Volume: 43 Pages: 797-800
  • DOI: 10.1038/ng.876
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• [Journal Article] Achievement of Sustained Viral Response after Switching Treatment from Pegylated Interferon alpha-2b to alpha-2a and Ribavirin in Patients with Recurrence of Hepatitis C Virus Genotype 1 Infection after Liver Transplantation : A Case Report (2011)
  • Author(s): Kawaoka T, Hiraga N, Takahashi S, Takaki S, Tsuge M, Nagaoki Y, Hashimoto Y, Katamura Y, Miki D, Hiramatsu A, Waki K, Imamura M, Kawakami Y, Aikata H, Ochi H, Tashiro H, Ohdan H, Chayama K
  • Journal Title: Intervirology Volume: (Epub 25 Aug.)(Epub)
  • DOI: 10.1159/000328661
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• [Journal Article] Will there be an HCV meeting in 2020? Summary of the 17th international meeting on hepatitis C virus and related viruses (2011)
  • Author(s): Wakita T, Suzuki T, Evans MJ, Shimotohno K, Chayama K, Matsuura Y, Hijikata M, Moriishi K, Seya T, Enomoto N, Koike K, Kato N, Kanto T, Hotta H
  • Journal Title: Gastroenterology Volume: 141 Pages: e1-5
  • DOI: 10.1053/j.gastro.2011.05.027
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• [Journal Article] Effects of hepatitis B virus infection on the interferon response in immunodeficient human hepatocyte chimeric mice (2011)
  • Author(s): Tsuge M, Takahashi S, Hiraga N, Fujimoto Y, Zhang Y, Mitsui F, Abe H, Kawaoka T, Imamura M, Ochi H, Hayes CN, Chayama K
  • Journal Title: J Infect Dis Volume: 204 Pages: 224-228
  • DOI: 10.1093/infdis/jir247
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• [Journal Article] Treatment strategy for early hepatocellular carcinomas : comparison of radiofrequency ablation with or without transcatheter arterial chemoembolization and surgical resection (2011)
  • Author(s): Tashiro H, Aikata H, Waki K, Amano H, Oshita A, Kobayashi T, Tanimoto Y, Kuroda S, Tazawa H, Chayama K, Asahara T, Ohdan H
  • Journal Title: J Surg Oncol Volume: 104 Pages: 3-9
  • DOI: 10.1002/jso.21745
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• [Journal Article] Antiviral effects of peginterferon alpha-2b and ribavirin following 24-week monotherapy of telaprevir in Japanese hepatitis C patients (2011)
  • Author(s): Ozeki I, Akaike J, Karino Y, Arakawa T, Kuwata Y, Ohmura T, Sato T, Kamiya N, Yamada I, Chayama K, Kumada H, Toyota J
  • Journal Title: J Gastroenterol Volume: 46 Pages: 929-937
  • DOI: 10.1007/s00535-011-0411-0
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• [Journal Article] Impact of ribavirin dose reduction on the efficacy of pegylated interferon plus ribavirin combination therapy for elderly patients infected with genotype 1b and high viral loads (2011)
  • Author(s): Kohno H, Kouno H, Aimitsu S, Aisaka Y, Kitamoto M, Kawakami H, Chayama K
  • Journal Title: Hepatol Res Volume: 41 Pages: 626-634
  • DOI: 10.1111/j.1872-034X.2011.00811.x
  • Peer Reviewed
• [Journal Article] IL28B but not ITPA polymorphism is predictive of response to pegylated interferon, ribavirin, and telaprevir triple therapy in patients with genotype 1 hepatitis C (2011)
  • Author(s): Chayama K, Hayes CN, Abe H, Miki D, Ochi H, Karino Y, Toyota J, Nakamura Y, Kamatani N, Sezaki H, Kobayashi M, Akuta N, Suzuki F, Kumada H
  • Journal Title: J Infect Dis Volume: 204 Pages: 84-93
  • DOI: 10.1093/infdis/jir210
  • Peer Reviewed
• [Journal Article] ME3738 enhances the effect of interferon and inhibits hepatitis C virus replication both in vitro and in vivo (2011)
  • Author(s): Abe H, Imamura M, Hiraga N, Tsuge M, Mitsui F, Kawaoka T, Takahashi S, Ochi H, Maekawa T, Hayes CN, Tateno C, Yoshizato K, Murakami S, Yamashita N, Matsuhira T, Asai K, Chayama K
  • Journal Title: J Hepatol Volume: 55 Pages: 11-18
  • DOI: 10.1016/j.jhep.2010.10.017
  • Peer Reviewed
• [Journal Article] Clinical features and prognosis in patients with hepatocellular carcinoma that developed after hepatitis C virus eradication with interferon therapy (2011)
  • Author(s): Nagaoki Y, Aikata H, Miyaki D, Murakami E, Hashimoto Y, Katamura Y, Azakami T, Kawaoka T, Takaki S, Hiramatsu A, Waki K, Imamura M, Kawakami Y, Takahashi S, Chayama K
  • Journal Title: J Gastroenterol Volume: 46 Pages: 799-808
  • DOI: 10.1007/s00535-011-0384-z
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• [Journal Article] Significant regression of a cavernous hepatic hemangioma to a sclerosed hemangioma over 12 years : a case study (2011)
  • Author(s): Mlyaki D, Aikata H, Waki K, Murakami E, Hashimoto Y, Nagaoki Y, Katamura Y, Kawaoka T, Takaki S, Hiramatsu A, Imamura M, Takahashi S, Ohya T, Sakimoto H, Arihiro K, Chayama K
  • Journal Title: Nihon Shokakibyo Gakkai Zasshi Volume: 108 Pages: 954-961
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• [Journal Article] Cutaneous complications after transcatheter arterial treatment for hepatocellular carcinoma via the internal mammary artery : how to avoid this complication (2011)
  • Author(s): Kajiwara K, Kakizawa H, Takeuchi N, Toyota N, Hieda M, Ishikawa M, Tanitame K, Tani C, Suzuki T, Fujikawa K, Aikata H, Chayama K, Awai K
  • Journal Title: Jpn J Radiol Volume: 29 Pages: 307-315
  • DOI: 10.1007/s11604-011-0559-9
  • Peer Reviewed
• [Journal Article] Prediction of response to peginterferon-alfa-2b plus ribavirin therapy in Japanese patients infected with hepatitis C virus genotype 1b (2011)
  • Author(s): Hashimoto Y, Ochi H, Abe H, Hayashida Y, Tsuge M, Mitsui F, Hiraga N, Imamura M, Takahashi S, Nelson Hayes C, Ohishi W, Kubo M, Tsunoda T, Kamatani N, Nakamura Y, Chayama K
  • Journal Title: J Med Virol Volume: 83 Pages: 981-988
  • DOI: 10.1002/jmv.22028
  • Peer Reviewed
• [Journal Article] Common genetic polymorphism of ITPA gene affects ribavirin-induced anemia and effect of peg-interferon plus ribavirin therapy (2011)
  • Author(s): Azakami T, Hayes CN, Sezaki H, Kobayashi M, Akuta N, Suzuki F, Kumada H, Abe H, Miki D, Tsuge M, Imamura M, Kawakami Y, Takahashi S, Ochi H, Nakamura Y, Kamatani N, Chayama K
  • Journal Title: J Med Virol Volume: 83 Pages: 1048-1057
  • DOI: 10.1002/jmv.22069
  • Peer Reviewed
• [Journal Article] Elimination of hepatitis C virus by short term NS3-4A and NS5B inhibitor combination therapy in human hepatocyte chimeric mice (2011)
  • Author(s): Ohara E, Hiraga N, Imamura M, Iwao E, Kamiya N, Yamada I, Kono T, Onishi M, Hirata D, Mitsui F, Kawaoka T, Tsuge M, Takahashi S, Abe H, Hayes CN, Ochi H, Tateno C, Yoshizato K, Tanaka S, Chayama K
  • Journal Title: J Hepatol Volume: 54 Pages: 872-878
  • DOI: 10.1016/j.jhep.2010.08.033
  • Peer Reviewed
• [Journal Article] IL-28B predicts response to chronic hepatitis C therapy--fine-mapping and replication study in Asian populations (2011)
  • Author(s): Ochi H, Maekawa T, Abe H, Hayashida Y, Nakano R, Imamura M, Hiraga N, Kawakami Y, Aimitsu S, Kao JH, Kubo M, Tsunoda T, Kumada H, Nakamura Y, Hayes CN, Chayama K
  • Journal Title: J Gen Virol Volume: 92 Pages: 1071-1081
  • DOI: 10.1099/vir.0.029124-0
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• [Journal Article] HCV replication animal model (2011)
  • Author(s): Imamura M, Hiraga N, Chayama K
  • Journal Title: Nihon Rinsho Volume: 69 Suppl 4 Pages: 75-79
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• [Journal Article] Impact of radiation and hepatitis virus infection on risk of hepatocellular carcinoma (2011)
  • Author(s): Ohishi W, Fujiwara S, Cologne JB, Suzuki G, Akahoshi M, Nishi N, Tsuge M, Chayama K
  • Journal Title: Hepatology Volume: 53 Pages: 1237-1245
  • DOI: 10.1002/hep.24207
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• [Journal Article] A single nucleotide polymorphism in activated Cdc42 associated tyrosine kinase 1 influences the interferon therapy in hepatitis C patients (2011)
  • Author(s): Fujimoto Y, Ochi H, Maekawa T, Abe H, Hayes CN, Kumada H, Nakamura Y, Chayama K
  • Journal Title: J Hepatol Volume: 54 Pages: 629-639
  • DOI: 10.1016/j.jhep.2010.07.021
  • Peer Reviewed
• [Journal Article] Factors predictive of sustained virological response following 72 weeks of combination therapy for genotype 1b hepatitis C (2011)
  • Author(s): Chayama K, Hayes CN, Yoshioka K, Moriwaki H, Okanoue T, Sakisaka S, Takehara T, Oketani M, Toyota J, Izumi N, Hiasa Y, Matsumoto A, Nomura H, Seike M, Ueno Y, Yotsuyanagi H, Kumada H
  • Journal Title: J Gastroenterol Volume: 46 Pages: 545-555
  • DOI: 10.1007/s00535-010-0358-6
  • Peer Reviewed
• [Presentation] Biological analysis of hepatitis C virus using human hepatocyte chimeric mouse (2012)
  • Author(s): 茶山一彰
  • Organizer: アジア太平洋肝臓病学会議(APASL)
  • Place of Presentation: 台北、台湾(招待講演)
  • Year and Date: 2012-02-19
• [Presentation] Dual Oral Combination Therapy with the NS5A Inhibitor BMS-790052 and the NS3 Protease Inhibitor BMS-650032 Achieved 90% Sustained Virologic Response (SVR12) in HCV Genotype 1b-Infected Null Responders (2011)
  • Author(s): 茶山一彰
  • Organizer: The 62nd Annual Meeting of American Association for the Study of Liver Diseases (AASLD)
  • Place of Presentation: アメリカ サンフランシスコ(招待講演)
  • Year and Date: 2011-11-07
• [Presentation] Biological analysis of hepatitis viruses using human hepatocyte chimeric mouse (2011)
  • Author(s): 茶山一彰
  • Organizer: 第59回国際歯科研究学会日本部会総会・学術大会(JADR2011)
  • Place of Presentation: 広島(招待講演)
  • Year and Date: 2011-10-09
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/naika1/