• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2011 Fiscal Year Final Research Report

Development of new gene transfer system for small fish

Research Project

  • PDF
Project/Area Number 22659148
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionYamaguchi University

Principal Investigator

SAKAIDA Isao  山口大学, 大学院・医学系研究科, 教授 (80263763)

Co-Investigator(Kenkyū-buntansha) TERAI Shuji  山口大学, 大学院・医学系研究科, 准教授 (00332809)
YAMAMOTO Naoki  山口大学, 大学教育機構, 講師 (90448283)
TAKAMI Taro  山口大学, 医学部附属病院, 助教 (60511251)
FUJISAWA Koichi  山口大学, 大学院・医学系研究科, 助教 (00448284)
Project Period (FY) 2010 – 2011
Keywords肝臓 / 遺伝子導入 / 線維化
Research Abstract

The possibility of gene therapy for zebrafish and medaka with hydrodynamic methods was examined. A condition setup for gene transfer was performed with GFP plasmid. We found few GFP positive cells in the liver. Gene transfer efficiency was small compared with the infusion of the mouse models.
We established fish live fibrosis model for evaluation of liver fibrosis improvement. We found that high fat diet and carbon tetrachloride is not suitable for liver fibrosis induction and DEN treatment for two months is suitable for liver fibrosis.

  • Research Products

    (3 results)

All 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (1 results)

  • [Journal Article] Ezetimibe reduces fatty acid quantity in liver and decreased inflammatory cell infiltration and improved NASH in medaka model2012

    • Author(s)
      Oishi T, Terai S, Kuwashiro S, Fujisawa K, Matsumoto T, Nishina H, Sakaida I
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 422 Pages: 22-27

    • Peer Reviewed
  • [Journal Article] Senescence marker protein 30(SMP30)/ regucalcin(RGN) expression decreases with aging, acute liver injuries and tumors in zebrafish2011

    • Author(s)
      Fujisawa K, Terai S, Hirose Y, Takami T, Yamamoto N, Sakaida I.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 414 Pages: 331-336

    • Peer Reviewed
  • [Presentation] SMP30/ Regucalcin is a useful marker for liver tumor ISHSR2011

    • Author(s)
      Koichi Fujisawa, Shuji Terai, Taro Takami, Naoki Yamamoto, Isao Sakaida
    • Place of Presentation
      フィレンツェイタリア
    • Year and Date
      2011-09-24

URL: 

Published: 2013-07-31  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi