2011 Fiscal Year Final Research Report
Identification of alveolar cell induction genes and differentiation of iPS cells into alveolar cells
| Project/Area Number |
22659160
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Hirosaki University |
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Principal Investigator |
SAIJYO Yasuo 弘前大学, 大学院・医学研究科, 教授 (10270828)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 非閉塞性肺疾患癌 / 肺線維症 / 呼吸器感染症 / その他 |
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| Research Abstract |
In this study, we tried to differentiate mouse iPS cells to alveolar cells in vitro. At first, iPS cells were cultured with ActinA and Wnt3a for 7 days. iPS cells differentiated into endodermal cells expressing Sox17 and Foxa2. iPS cells were further cultured with FGF2 for additional 5 days. Finally, about 8 % iPS cells differentiated into alveolar type II cells determined by SP-C expression and electron microscopy.
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