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2011 Fiscal Year Final Research Report

RNA modification and self-discrimination : A possible link to the pathogenesis of autoimmune disease

Research Project

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Project/Area Number 22659186
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeSingle-year Grants
Research Field 膠原病・アレルギー・感染症内科学
Research InstitutionUniversity of Miyazaki

Principal Investigator

KENMOCHI Naoya  宮崎大学, フロンティア科学実験総合センター, 教授 (00133124)

Co-Investigator(Renkei-kenkyūsha) TAKAYANAGI Atushi  慶應義塾大学, 医学部, 講師 (80245464)
Project Period (FY) 2010 – 2011
KeywordsRNA修飾 / ノンコーディングRNA / 抗RNA抗体 / リボソームRNA / snoRNA
Research Abstract

Non-coding RNAs undergo various posttranscriptional modifications. To investigate the physiological relevance of RNA modifications in autoimmune diseases, we generated artificial anti-RNA antibodies that bind to the epitope for patient autoantibodies and developed a zebrafish model of defective RNA modifications. Impaired RNA modification led to severe morphological defects and embryonic lethality in zebrafish, which suggests that RNA modifications play an important role in vertebrate development.

  • Research Products

    (21 results)

All 2012 2011 2010 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (10 results) Book (1 results) Remarks (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Dissecting the protein-RNA interface : the role of protein surface shapes and RNA secondary structures in protein-RNA recognition2012

    • Author(s)
      Iwakiri, J., Tateishi, H., Chakraborty, A., Patil, P. and Kenmochi, N.
    • Journal Title

      Nucleic Acids Res

      Volume: 40 Pages: 3299-3306

    • Peer Reviewed
  • [Journal Article] Loss of ribosomal RNA modification causes developmental defects in zebrafish2012

    • Author(s)
      Higa-Nakamine, S., Suzuki, T., Uechi, T., Chakraborty, A., Nakajima, Y., Nakamura, M., Hirano, N., Suzuki, T. and Kenmochi, N.
    • Journal Title

      Nucleic Acids Res

      Volume: 40 Pages: 391-398

    • Peer Reviewed
  • [Journal Article] リボソーム病とp53経路2011

    • Author(s)
      剣持直哉
    • Journal Title

      医学のあゆみ(RNA医学・医療)

      Volume: 238 Pages: 476-480

  • [Journal Article] Guarding the' Translation Apparatus': Defective ribosome biogenesis and the p53 signaling pathway2011

    • Author(s)
      Chakraborty, A., Uechi, T. and Kenmochi, N.
    • Journal Title

      Wiley Interdiscip Rev RNA

      Volume: 2 Pages: 507-522

    • Peer Reviewed
  • [Journal Article] Erythropoiesis failure due to RPS19 deficiency is independent of an activated p53 response in a zebrafish model of Diamond-Blackfan anaemia2011

    • Author(s)
      Torihara, H., Uechi, U., Chakraborty, A., Shinya, M., Sakai N. and Kenmochi, N.
    • Journal Title

      Brit. J. Haematol

      Volume: 152 Pages: 648-654

    • Peer Reviewed
  • [Journal Article] Screening of scFv-displaying phages recognizing distinct extracellular domains of EGF receptor by target-guided proximity labeling method2011

    • Author(s)
      Chang, C., Takayanagi, A., Yoshida, T. and Shimizu, N.
    • Journal Title

      J. Immunol. Methods

      Volume: 372 Pages: 127-136

    • Peer Reviewed
  • [Presentation] Loss of snoRNA expression impairs ribosomal RNA modification and causes developmental defects in zebrafish.2012

    • Author(s)
      N. Kenmochi, S. Higa-Nakamine, T. Uechi, A. Chakraborty, Y. Nakajima, T. Suzuki and T. Suzuki
    • Organizer
      Protein-RNA Interactions in Biology and Disease
    • Place of Presentation
      Santa Fe
    • Year and Date
      20120304-09
  • [Presentation] リボソームの異常と赤血球形成不全:ゼブラフィッシュを用いた解析2011

    • Author(s)
      上地珠代, 中島由香里, 鳥原英嗣, 剣持直哉
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20111213-16
  • [Presentation] Development of transgenic zebrafish using RNAi technology to investigate the role of ribosome in cancer2011

    • Author(s)
      G. V. Yadav, A. Chakraborty, T. Uechi and N. Kenmochi
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20111213-16
  • [Presentation] When p53 senses faulty ribosomes : Dynamics of c-Myc target nucleolar proteins and their correlation with Tp53 response in Rpl11-deficient zebrafish2011

    • Author(s)
      A. Chakraborty, T. Uechi and N. Kenmochi
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20111213-16
  • [Presentation] Efficient Production of Recombinant Antibodies in Mammalian Cells by Transient Transfection of Expression Vectors with 2.5K "PEI-MAX"2011

    • Author(s)
      高柳淳, 吉田徹彦, 清水信義
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      20111213-16
  • [Presentation] ゼブラフィッシュをモデルとしたリボソーム病発症の分子機構の解析2011

    • Author(s)
      剣持直哉
    • Organizer
      第84回日本生化学会大会(シンポジウム招待講演)
    • Place of Presentation
      京都
    • Year and Date
      20110921-24
  • [Presentation] Ribosomal stress and the p53 network : An activated Tp53 response in Rpl11-deficient. zebrafish correlates with an upregulated c-Myc activity2011

    • Author(s)
      A. Chakraborty, T. Uechi and N. Kenmochi
    • Organizer
      Protein Synthesis and Translational Control
    • Place of Presentation
      Heidelberg
    • Year and Date
      20110907-11
  • [Presentation] Ribosomal integrity and p53 pathway : Induction of Tp53 in Rpl11-deficient zebrafish correlates with enhanced transcription of c-Myc and its target genes involved in ribosome biogenesis2011

    • Author(s)
      A. Chakraborty, T. Uechi and N. Kenmochi
    • Organizer
      RNA 2011 : Sixteenth Annual Meeting of the RNA Society
    • Place of Presentation
      Kyoto
    • Year and Date
      20110614-18
  • [Presentation] Defective erythropoiesis and ribosomal dysfunction in zebrafish model of Diamond-Blackfan anemia2011

    • Author(s)
      T. Uechi, Y. Nakajima, H. Torihara and N. Kenmochi
    • Organizer
      RNA 2011 : Sixteenth Annual Meeting of the RNA Society
    • Place of Presentation
      Kyoto
    • Year and Date
      20110614-18
  • [Presentation] ヒトゲノム情報と人工抗体ライブラリーを活用したプロテオーム解析の新戦略2010

    • Author(s)
      高柳淳
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      20101213-16
  • [Book] Ribosomes and ribosomal proteins : more than just 'housekeeping'.2012

    • Author(s)
      Chakraborty, A. and Kenmochi, N
    • Publisher
      John Wiley & Sons Ltd
  • [Remarks] (1) snoRNAデータベース

    • URL

      http://snoopy.med.miyazaki-u.ac.jp/

  • [Remarks] (2) RPGデータベース

    • URL

      http://ribosome.med.miyazaki-u.ac.jp/

  • [Remarks] (3)ホームページ

    • URL

      http://ribosome.med.miyazaki-u.ac.jp/labo/

  • [Patent(Industrial Property Rights)] 抗RNA抗体のスクリーニング方法及びRNA抗体の製造方法2011

    • Inventor(s)
      剣持直哉、吉浜麻生、高柳淳、清水信義
    • Industrial Property Rights Holder
      宮崎大学、慶應義塾
    • Industrial Property Number
      特願、2011-098698
    • Filing Date
      2011-04-26

URL: 

Published: 2013-07-31  

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