2010 Fiscal Year Annual Research Report
コレシストキニン塗布によるかゆみの制御と新規治療薬への応用
| Project/Area Number |
22659208
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
戸倉 新樹 浜松医科大学, 医学院, 教授 (00172156)
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| Co-Investigator(Kenkyū-buntansha) |
森 智子 産業医科大学, 医学部, 助教 (80399203)
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| Keywords | かゆみ / コレシストキニン / CCK2R / サブスタンスP / そう痒治療薬 / 肥満細胞 |
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| Research Abstract |
かゆみ惹起神経ペプチドであるサブスタンスP(SP)刺激によってコレシストキニン受容体であるCCK2発現元進がみられたことは、CCKがCCK2Rシグナリングを介して、負の制御としてかゆみ抑制作用を示す可能性を示唆した。ICRマウスの背部を剃毛し、そこにSP(100nm/部分)を皮下注し、マウスの後肢掻破行動を誘導した。SP皮下投与10分前に剃毛部に種々のCCKペプチドを塗布し、溶液のみのコントロールと比べたところ、掻破回数がとくにCCK8Sで減弱した。CCK皮下投与でも同様の効果をもたらすか、SPと同時にCCK8Sを皮下投与したところ掻破回数が減弱した。皮下投与でもかゆみ抑制効果を示すのであれば、角化細胞以外に肥満細胞も標的細胞となることが示唆された。マウス胎仔由来肥満細胞をcompound 48/80で脱顆粒刺激する系で、CCK8Sを添加したところ脱顆粒は抑制された。以上のことから、CCKはかゆみを抑制する能力をもち、少なくとも肥満細胞を機能を抑制して効果を示すのではないかと考えられ、創薬への端緒となった。
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[Journal Article] Comparison of skin barrier function and sensory nerve electric current perception threshold between IgE-high extrinsic and IgE-normal intrinsic types of atopic dermatitis2010
Author(s)
Mori T, Ishida K, Mukumoto S, Yamada Y, Imokawa G, Kabashima K, Kobayas hi M, Bito T, Nakamura M, Ogasawara K, Tokura Y
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Journal Title
Br J Dermatol
Volume: 162
Pages: 83-90
Peer Reviewed
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[Journal Article] Flaky tail mouse denotes human atopic dermatitis in the steady state and by topical application with Dermatophagoides pteronyssimus extract2010
Author(s)
Moniaga CS, Egawa G, Kawasaki H, Chikuma M, Honda T, Tanizaki H, Nakajima S, Matsuoka H, Kubo A, Tokura Y, Miyachi Y, Amagai M, Kabashima K
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Journal Title
Am J Pathol
Volume: 176
Pages: 2385-2393
Peer Reviewed
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[Journal Article] Activated regulatory T cells are the major T cell type emigrating from the skin during a cutaneous immune response in mice2010
Author(s)
Tomura M, Honda T, Tanizaki H, Otsuka A, Egawa G, Tokura Y, Waldmann H, Hon S, Cyster JG, Watanabe T, Miyachi Y, Kanagawa O, Kabashima K
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Journal Title
J Clin Invest
Volume: 120
Pages: 883-893
Peer Reviewed
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[Journal Article] Acta Derm Venereol2010
Author(s)
Tanizaki H, Matsumura Y, Tokura Y, Miyachi Y, Kabashima K
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Journal Title
A case of suppurative granuloma induced by insulin injection
Volume: 90
Pages: 541-542
Peer Reviewed
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[Journal Article] Acute hepatic failure secondary to extensive hepatic replacement by metastatic a melanotic melanoma : an autopsy case report2010
Author(s)
Fusasaki T, Narita R, Hiura M, Abe S, Tabaru A, Hino R, Matsuyama A, Shimajiri S, Tokura Y, Sasaguri Y, Harada M
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Journal Title
Clin J Gastroenterol
Volume: 3
Pages: 327-331
Peer Reviewed
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[Journal Article] J UOEH2010
Author(s)
Haruyama S, Sugita K, Kawakami C, Nakamura M, Tokura Y
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Journal Title
Spinal cord compression presumably due to metastasized cutaneous squamous cell carcinoma to the thoracic spine
Volume: 32
Pages: 155-159
Peer Reviewed
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[Journal Article] 塩酸オロパタジン内服によるアトピー性皮膚炎患者の血中サブSタンスPとTh17細胞割合への影響2010
Author(s)
椛島利江子, 尾藤利憲, 田尻真貴子, 川上千佳, 深町晶子, 吉木竜太郎, 杉田和成, 日野亮介, 森智子, 小林美和, 中村元信, 戸倉新樹
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Journal Title
西日本皮膚科
Volume: 72
Pages: 159-161
Peer Reviewed
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[Journal Article] The type of skin eruptions is an independent prognostic indicator for adult T-cell leukemia/lymphoma
Author(s)
Sawada Y, Hino R, Hama K, Ohmori S, Fueki H, Yamada S, Fukamachi S, Tajiri M, Kumo R, Yoshioka M, Nakashima D, Sugita K, Yoshiki R, Shimauchi T, Mori T, Izu K, Kobayashi M, Nakamura M, Tokura Y
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Journal Title
Peer Reviewed
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[Presentation] Barrier function-based immunological differences between extrinsic and intrinsic types of atopic dermatitis2010
Author(s)
Kabashima R, Nakamura M, Sakabe J, Fukamachi S, Sugita K, Hino R, Mori T, Kobayashi M, Bito T, Kabashima K, Nomura Y, Akiyama M, Shimizu H, Tokura Y
Organizer
The 35th Annual Meeting of the Japanese Society for Investigative Dermatology
Place of Presentation
Wakayama Prefectural Cultural Hall, Wakayama?
Year and Date
20101203-20101205
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[Presentation] Inhibition of immediate and late-phase cutaneous reactions by blockade of D1-like dopamine receptors2010
Author(s)
Mori T, Kabashima K, Kuroda E, Sakabe J, Fukamachi S, Kobayashi M, Nakajima S, Nakano K, Tanaka Y, Yamashita S, Tokura Y
Organizer
The 14th International Congress of immunology
Place of Presentation
Kobe Convention Center Complex?
Year and Date
20100822-20100827
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[Presentation] The intrinsic type of atopic dermatitis shows normal barrier function, lack of filaggrin mutations, high percentage of Thl cells, and high frequency of metal allergy2010
Author(s)
Kabashima R, Nakamura M, Kobayashi M, Bito T, Kabashima K, Nomura Y, Akiyama M, Shimizu H, Tokura Y
Organizer
The 70^<th> Annual Meeting of Society for Investigative Dermatology
Place of Presentation
Hilton Atlanta Hotel, Atlanta, Georgia?
Year and Date
20100505-20100508
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