Analysis of L1 retrotransposition activity in the brains of patients with psychiatric disorders.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22659214
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Psychiatric science
• Research Institution: The University of Tokyo (2011-2012); The Institute of Physical and Chemical Research (2010)
• Principal Investigator: IWAMOTO Kazuya 東京大学, 医学部附属病院, 特任准教授 (40342753)
• Project Period (FY): 2010 – 2012
• Keywords: 死後脳 / 神経細胞 / トランスポゾン / 統合失調症 / 精神疾患

Research Abstract

We measured LINE-1 copy number in brains of patients with psychiatric disorders by Taqman-based PCR. We identified significant increase of LINE-1 copy number in brains of patients with schizophrenia in two different cohorts. This finding is well concordant with neurodevelopmental model of schizophrenia.

Research Products

• [Journal Article] Neuronal cell-type specific DNA methylation patterns of the Cacna1c gene. (2013)
  • Author(s): Nishioka Masaki, Shimada Takafumi, Bundo Miki, Ukai Wataru, Hashimoto Eri, Saito Toshikazu, Kano Yukiko, Sasaki Tsukasa, Kasai Kiyoto, Kato Tadafumi, Iwamoto Kazuya.
  • Journal Title: International Journal of Developmental Neuroscience Volume: 31 Pages: 89-95.
  • DOI: doi:10.1016/j.ijdevneu.
  • Peer Reviewed
• [Journal Article] Comprehensive DNA methylation analysis of peripheral blood cells derived from patients with first-episode schizophrenia. (2013)
  • Author(s): Nishioka Masaki, Bundo Miki, Koike Shinsuke, Takizawa Ryuichi, Kakiuchi Chihiro, Araki Toshiyuki, Kasai Kiyoto, Iwamoto Kazuya.
  • Journal Title: Journal of Human Genetics Volume: 58 Pages: 91-97
  • DOI: DOI:doi:10.1038/jhg.2012.140
  • Peer Reviewed
• [Journal Article] DNA methylation in schizophrenia: progress and challenges of epigenetic studies. (2012)
  • Author(s): Nishioka Masaki, Bundo Miki, Kasai Kiyoto, Iwamoto Kazuya.
  • Journal Title: Genome Medicine Volume: 4 Pages: 96
  • DOI: DOI:doi:10.1186/gm397
  • Peer Reviewed
• [Journal Article] A systematic evaluation of whole genome amplification of bisulfite-modified DNA. (2012)
  • Author(s): Bundo Miki, Sunaga Fumiko, Ueda Junko, Kasai Kiyoto, Kato Tadafumi, Iwamoto Kazuya.
  • Journal Title: Clinical Epigenetics Volume: 4 Pages: 22
  • DOI: DOI:doi:10.1186/1868-7083-4-22
  • Peer Reviewed
• [Journal Article] DNA methylation of the BDNF gene and its relevance to psychiatric disorders
  • Author(s): Ikegame Tempei, Bundo Miki, Murata Yui, Kasai Kiyoto, Kato Tadafumi, Iwamoto Kazuya
  • Journal Title: Journal of Human Genetics Volume: (印刷中)
  • DOI: doi: 10.1038/jhg.2013.65.
  • Peer Reviewed
• [Presentation] Neuronal epigenomics in schizophrenia. (2012)
  • Author(s): Iwamoto Kazuya(代表), Bundo Miki, Kasai Kiyoto, Kato Tadafumi.
  • Organizer: 第35回日本神経科学会(招待講演)
  • Place of Presentation: 名古屋国際会議場、愛知
  • Year and Date: 20120918-0921
• [Presentation] 双極性障害および統合失調症患者神 経細胞核におけるエピゲノム解析 (2012)
  • Author(s): 岩本和也(代表)、文東美紀、上田順子、 須永史子、根本妙子、笠井清登、加藤忠 史
  • Organizer: 第回エピジェネティクス研究会年会
  • Place of Presentation: 東京一ツ橋学術総合セ ンター、東京
  • Year and Date: 20120514-0515
• [Presentation] Epigenomics of brains of patients with bipolar disorder and schizophrenia.3rd Shanghai International Conference of Epigenetics and Diseases (2012)
  • Author(s): Iwamoto Kazuya(代表), Kato Tadafumi.
  • Organizer: 7th Annual Conference of Asian Epigenome Alliance/Genome Medicine Workshop onEpigenetic(-omic)s in Diseases(招待 講演)
  • Place of Presentation: Shanghai,China
  • Year and Date: 20120514-0515
• [Presentation] 精神疾患患者末梢試料を用いたエピゲノム解析の可能性 (2012)
  • Author(s): 岩本和也
  • Organizer: 第34回日本生物学的精神医学会(招待講演)
  • Place of Presentation: 神戸ポートアイランド、兵庫
  • Year and Date: 2012-09-28
• [Presentation] 精神疾患患者試料を用いた epigenome 解析 (2011)
  • Author(s): 岩本和也
  • Organizer: 大阪大学蛋白質研究所 セミナー(招待講演)
  • Place of Presentation: 大阪大学、大阪
  • Year and Date: 20111117-1118
• [Presentation] Bundo Miki, Kasai Kiyoto, Kato Tadafumi. DNA methylation analysis of neuronal nuclei derived from bipolar disorder. (2011)
  • Author(s): Iwamoto Kazuya(代表 )
  • Organizer: 第34回日本神経科学会(招待講演)
  • Place of Presentation: パシフィコ横浜、神奈川
  • Year and Date: 20110914-0917
• [Presentation] 脳ゲノムの多様性と精神疾患 (2011)
  • Author(s): 岩本和也(代表)、文東美紀、笠井清登、 加藤忠史
  • Organizer: 第33回日本生物学的精神医学会
  • Place of Presentation: ホテルグランパシフィ ック LE DAIBA、東京
  • Year and Date: 20110521-0522
• [Presentation] Epigenetics of bipolar disorder. (2011)
  • Author(s): Iwamoto Kazuya(代表), Kato Tadafumi.
  • Organizer: Epigenetics in clinical Medicine(招 待講演)
  • Place of Presentation: Stockholm,Sweden
  • Year and Date: 20110404-0406
• [Presentation] 精神疾患患者死後脳における エピゲノム解析 (2010)
  • Author(s): 岩本和也
  • Organizer: 第20回日本臨床精神 薬理学会・第40回日本神経精神薬理学会(招待講演)
  • Place of Presentation: 仙台国際センター、宮城
  • Year and Date: 20100915-0917
• [Presentation] Epigenome analysis of brains of patients with bipolar disorder. (2010)
  • Author(s): Iwamoto Kazuya(代表). Kato Tadafumi.
  • Organizer: 2nd Meeting of East Asian Bipolar Forum(招待講演)
  • Place of Presentation: Kyusyu,Japan
  • Year and Date: 20100907-0908