2011 Fiscal Year Final Research Report
Screening of hypertrophic differentiation-related factors for innovative research of osteoarthritis.
| Project/Area Number |
22659266
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKETOMI Shuji 東京大学, 医学部附属病院, 助教 (70570018)
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| Co-Investigator(Kenkyū-buntansha) |
TAKESHITA Katsushi 東京大学, 医学部附属病院, 准教授 (30262009)
NAKAGAWA Takumi 東京大学, 医学部附属病院, 講師 (90338385)
MASUDA Kazuhiro 東京大学, 医学部附属病院, 助教 (70407948)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 関節病学 |
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| Research Abstract |
For establishment of a fluorescence monitoring system to detect hypertrophic differentiation of chondrocytes, we analyzed the promoter regions of the representative hypertrophic marker genes including COL10A1, VEGF and MMP13, and indentified the core enhancers in these genes. We constructed the reporter vectors by ligating the tandem-repeated core enhancers, the basal promoter sequences and fluorescent proteins, and lentivirally introduced them into ATDC5 cells. We performed the expression cloning by these reporter cells, and identified several positive clones which were still under analyses.
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