2011 Fiscal Year Final Research Report
Identification of cell-of-origin of sarcomas using human iPS cells containing inducible expression vectors
| Project/Area Number |
22659272
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KATO Tomohisa 京都大学, 再生医科学研究所, 講師 (50301247)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 骨軟部肉腫 / 人工多能性幹細胞 / 遺伝子発現誘導 / 融合遺伝子 |
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| Research Abstract |
The object of this project is to identify the cell-of-origin of sarcomas using human iPS cells. Using drug-inducible expression vector and transposes-supported gene introduction system, we successfully established genetically modified iPS cells. Without drug-treatment these cells retained undifferentiated state. When the SYT-SSX gene was induced, however, the expression level of stem cell markers were down-regulated and at the same time those of lineage-specific genes were up-regulated, suggesting the involvement of SYT-SX protein in the process of differentiation.
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Research Products
(22 results)
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[Journal Article] Prostaglandin E2 receptor type 2 receptor-selective agonist prevents the degeneration of articular cartilage in rabbit knees with traumatic instability2011
Author(s)
Mitsui H, Aoyama T, Furu M, Ito K, Jin Y, Maruyama T, Kanaji T, Fujimura S, Sugihara H, Nishiura A, Otsuka T, Nakamura T, Toguchida J
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Journal Title
Arthritis Res Ther
Volume: 13(5)
Pages: 146
URL
Peer Reviewed
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[Journal Article] Identification of AFAP1L1 as a prognostic marker for spindle cell sarcomas2011
Author(s)
Furu M, Kajita Y, Nagayama S, Ishibe T, Shima Y, Nishijo K, Uejima D, Takahashi R, Aoyama T, Nakayama T, Nakamura T, Nakashima Y, Ikegawa M, Imoto S, Katagiri T, Nakamura Y, Toguchida J
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Journal Title
Oncogene
Volume: 30(38)
Pages: 4015-25
DOI
Peer Reviewed
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[Journal Article] Involvement of cancer biomarker C7orf24 in the growth of human osteosarcoma2011
Author(s)
Uejima D, Nishijo K, Kajita Y, Ishibe T, Aoyama T, Kageyama, Iwaki H, Nakamura T, Iida, Yoshiki T, Toguchida J
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Journal Title
Anticancer Res
Volume: 31(4)
Pages: 1297-305
URL
Peer Reviewed
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[Journal Article] Gene Expression Profiling of Synovial Sarcoma : Distinct Signature of Poorly Differentiated Type2010
Author(s)
Nakayama R, Mitani S, Nakagawa T, Hasegawa T, Kawai A, Morioka H, Yabe H, Toyama Y, Ogose A, Nakayama, T, Toguchida, J, Yoshida T, Ichikawa H
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Journal Title
Am J Surg Pathol
Volume: 34(11)
Pages: 1599-607
URL
Peer Reviewed
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[Journal Article] Histone modifiers, YY1 and p300, regulate the expression of cartilage-specific gene, chondromodulin-I, in mesenchymal stem cells2010
Author(s)
Aoyama T, Okamoto T, Fukiage K, Otsuka S, Furu M, Ito K, Jin Y, Ueda M, Nagayama S, Nakayama T, Nakamura T, Toguchida J
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Journal Title
J Biol Chem
Volume: 285(39)
Pages: 29842-50
DOI
Peer Reviewed
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