Exploration for a new biomarker of prostate cancer using microRNA secreted in urine samples

2011 Fiscal Year Final Research Report

• Project/Area Number: 22659288
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Kyoto University
• Principal Investigator: OGAWA Osamu 京都大学, 医学研究科, 教授 (90260611)
• Co-Investigator(Kenkyū-buntansha): YOSHIMURA Koji 京都大学, 医学研究科, 准教授 (40397542) ; KANBA Tomomi 京都大学, 医学研究科, 講師 (20402836) ; INOUE Takahiro 京都大学, 医学研究科, 助教 (80511881)
• Project Period (FY): 2010 – 2011
• Keywords: マイクロRNA / 前立腺癌 / 去勢抵抗性

Research Abstract

In this research, we hypothesized that microRNAs were secreted in urine from prostate cancer cells, and that the microRNAs, which are specific for prostate cancer, could be stably extracted from urine samples, and could be useful for new biomarkers. We performed microRNA array using whole urine samples, but none was significantly different in expression. We found that the microRNA expression in culture medium could reflect its expression in cell lines. But the expressions of some microRNAs, which have already been known to be associated with prostate cancer, were different in culture medium, cell lines, and urine samples. We are trying to establish the proper method, by which we can precisely estimate the expression of microRNAs in urine samples, and we are planning to explore new microRNAs associated with prostate cancer.

Research Products

• [Journal Article] Roleof Signaling Transduction Pathways inDevelopment of Castration-ResistantProstate Cancer (2011)
  • Author(s): Takahiro Inoue, Osamu Ogawa
  • Journal Title: Prostate Cancer, vol.2011, Article ID Volume: 647987 Pages: 7
  • DOI: doi:10.1155/2011/647987.
• [Journal Article] Long-term outcomes of three-dimensional conformal radiation therapy combined with neoadjuvant hormonal therapy for Japanese patients with T1c-T2N0M0 prostate cancer (2011)
  • Author(s): Mizowaki T, Takayama K, Norihisa Y, Ogura M, Kamba T, Inoue T, Shimizu Y, Kamoto T, Ogawa O, Hiraoka M
  • Journal Title: Int J Clin Oncol
  • DOI: doi:10.1007/s10147-011-0326-z
• [Journal Article] A potent chemotherapeutic strategy inprostate cancer (2011)
  • Author(s): Xing ND, Ding ST, Saito R, Nishizawa K, Kobayashi T, Inoue T, OishiS, Fujii N, Lv JJ, Ogawa O, Nishiyama H
  • Journal Title: S-(methoxytrityl)-l-cysteine, a novel Eg5inhibitor. Asian J Androl Volume: 13 Pages: 236-41
  • DOI: doi:10.1038/aja.2010.171
• [Journal Article] SPA-1 controls the invasionand metastasis of human prostate cancer (2011)
  • Author(s): Shimizu Y, Hamazaki Y, Hattori M, Doi K, Terada N, Kobayashi T, Toda Y, Yamasaki T, Inoue TA, Kajita Y, Maeno A, Kamba T, Mikami Y, Kamoto T, Yamada T, Kanno T, Yoshikawa K, Ogawa O, Minato N, Nakamura E.
  • Journal Title: Cancer Sci Volume: 102 Pages: 828-36
  • DOI: doi:10.1111/j.1349-7006.2011.01876.x
• [Journal Article] Activationof Rac1 Is Closely Related toAndrogen-Independent Cell Proliferationof Prostate Cancer Cells Both in Vitro andin Vivo (2010)
  • Author(s): Kobayashi T, Inoue T, Shimizu Y, Terada N, Maeno A, Kajita Y, Yamasaki T, Kamba T, Toda Y, Mikami Y, Yamada T, KamotoT, Ogawa O, Nakamura E
  • Journal Title: Mol Endocrinol Volume: 24 Pages: 722-34
  • DOI: doi:10.1210/me.2009-0326
• [Journal Article] Identification of EP4 as a Potential Target for the Treatment of Castration-Resistant Prostate Cancer Using a Novel Xenograft Model (2010)
  • Author(s): Terada N, Shimizu Y, Kamba T, Inoue T, Maeno A, Kobayashi T, Nakamura E, Kamoto T, Kanaji T, Maruyama T, Mikami Y, Toda Y, Matsuoka T, Okuno Y, Tsujimoto G, Narumiya S, Ogawa O
  • Journal Title: Cancer Res Volume: 70 Pages: 1606-15
  • DOI: doi:10.1158/0008-5472.CAN-09-2984
• [Journal Article] Regulation of androgen receptor transactivity and mTOR-S6 kinase pathway by Rheb in prostate cancer cell proliferation (2010)
  • Author(s): Takashi Kobayashi, Yosuke Shimizu, Naoki Terada, Toshinari Yamasaki, Eijiro Nakamura, Yoshinobu Toda, Hiroyuki Nishiyama, Toshiyuki Kamoto, Osamu Ogawa and Takahiro Inoue
  • Journal Title: Prostate Volume: 70 Pages: 866-874
  • DOI: doi:10.1002/pros.21120
• [Presentation] Exploration and novel identification of microRNAs associated with bladder cancer by miRNA chip array profiling (2011)
  • Author(s): 中嶋正和
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-11-04
• [Presentation] Focal adhesion kinase activity correlates with biochemical recurrence after radical prostatectomy (2011)
  • Author(s): 井上貴博
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-04
• [Presentation] miR-582-5p and miR-96 induces androgen-independent growth of prostate cancer cells (2011)
  • Author(s): 前野淳
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-04
• [Presentation] RalGAPによる腫瘍浸潤転移抑制機能の新規同定 (2011)
  • Author(s): 斎藤亮一
  • Organizer: 日本泌尿器科学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-04-22
• [Remarks]
  • URL: http://www.urology.kuhp.kyoto-u.ac.jp/