Identification of Cervical Cancer Stem Cells and development of the new method for treatment of that cells as the target.

2011 Fiscal Year Final Research Report

• Project/Area Number: 22659296
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: University of Toyama
• Principal Investigator: NIKAIDO Toshio 富山大学, 大学院・医学薬学研究部(医学), 教授 (50180568)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Toshiko 富山大学, 大学院・医学薬学研究部(医学), 准教授 (00171421) ; OKABE Motonori 富山大学, 大学院・医学薬学研究部(医学), 助教 (60283066) ; KOIKE Chika 富山大学, 大学院・医学薬学研究部(医学), 助教 (10523889) ; HIDAKA Takao 富山大学, 大学院・医学薬学研究部(医学), 准教授 (70283083) ; NAKASHIMA Akitoshi 富山大学, 大学院・医学薬学研究部(医学), 助教 (00436792)
• Project Period (FY): 2010 – 2011
• Keywords: 婦人科腫瘍学 / 癌幹細胞 / 組織幹細胞 / 婦人科組織 / 幹細胞マーカー / 組織アレイ

Research Abstract

It is concluded that CD44+CD24+sub-population cells have the in vitro and in vivo characteristics of cancer stem cells and their specific detection could be highly valuable for the therapy of this recurrent disease by screening of new anticancer drugs.

Research Products

• [Journal Article] Use of a hyper-dried cross-linked amniotic membrane as initial therapy for corneal perforations (2011)
  • Author(s): Kitagawa K., Okabe M., Yanagisawa S., Zhang Xue-Yun., Nikaido T., and Hayashi A.
  • Journal Title: Jpn. J. Ophthalmol Volume: 55 Pages: 16-21
  • Peer Reviewed
• [Journal Article] Effect of hyperdry amniotic membrane patches attached over the bony surface of mastoid cavities in canal wall down tympanoplasty (2011)
  • Author(s): Shojaku H., Takakura H., Okabe M., Fujisaka M., Watanabe Y., and Nikaido T.
  • Journal Title: Laryngoscope Volume: 121(9) Pages: 1953-1957
  • Peer Reviewed
• [Journal Article] Cultured epithelial grafting using human amniotic membrane : the potential for using human amniotic epithelial cells as a cultured oral epithelium sheet (2011)
  • Author(s): Koike T., Yasuo M., Shimane T., Kobayashi H., Nikaido T., and Kurita H.
  • Journal Title: Arch. Oral Biol Volume: 56(10) Pages: 1170-1176
  • Peer Reviewed
• [Journal Article] Traditional Japanese formula kigikenchuto accelerates healing of pressure-loading skin ulcer in rats. Evid Based Complement Altemat Med (2011)
  • Author(s): Kimura M., Shibahara N., Hikiami H., Yoshida T., Jo M., Kaneko M., Mogami T., Fujimoto M., Goto H., and Shimada Y.
  • Journal Title: Volume 2011 Volume: 9 Pages: 592791
  • Peer Reviewed
• [Journal Article] Mechanisms of collagen fibril aligment in tendon injury : From tendon regeneration to artificial tendon (2011)
  • Author(s): Torigoe K., Tanaka HF., Yonenaga K., Ohkochi H., Miyasaka M., Sato R., Kusumaki T., Yoshida K., and Yoshida T.
  • Journal Title: J. Orhop. Res Volume: 29 Pages: 1944-1950
  • Peer Reviewed
• [Journal Article] Hyaluronan tetrasaccharide promotes regeneration of peripheral nerve : in vivo analysis by film method (2011)
  • Author(s): Torigoe k., Tanak HF., Ohkochi H., Miyasaka M., Yamanokuchi H., Yoshida K., and Yoshida T.
  • Journal Title: Brain Res Volume: 18, 1385 Pages: 87-92
  • Peer Reviewed
• [Journal Article] Clinical Application of a Hyperdry Amniotic Membrane on Surgical Defects of the Oral Mucosa (2011)
  • Author(s): Arai N., Tsuno H., Okabe M., Yoshida T., Koike C., Noguchi M., and Nikaido T.
  • Journal Title: J Oral Maxillofac Surg
  • Peer Reviewed
• [Journal Article] Establishment of Immortalized Human Amniotic Mesenchymal Stem Cells (2011)
  • Author(s): Teng Z., Yoshida T., Okabe M., Toda A., Higuchi O., Nogami M., Yoneda N., Zhou K., Kyo S., Kiyono T., and Nikaido T.
  • Journal Title: Cell Transplant Volume: (in press)
  • Peer Reviewed
• [Journal Article] New dried human amniotic membrane is useful as a substitute for dural repair after skull base surgery (2011)
  • Author(s): Tomita T., Hayashi N., Okabe M., Yoshida T., Hamada H., Endo S., and Nikaido T.
  • Journal Title: Skull Base Volume: (in press)
  • Peer Reviewed
• [Journal Article] Prediction of exact delivery time in patients with preterm labor and intact membranes at admission by amniotic fluid interleukin-8 level and preterm labor index (2011)
  • Author(s): Yoneda S., Shiozaki A., Shima T., Ito M., Yamanaka M., Hidaka T., Sumi S., Saito S.
  • Journal Title: J. Obstet. Gynecol. Res Volume: 37 Pages: 861-866
  • Peer Reviewed
• [Journal Article] Future directions of studies for recurrent miscarriage associated with immune etiologies (2011)
  • Author(s): Saito S, Nakashima A. Shima T.
  • Journal Title: J Reprod Immunol Volume: 90 Pages: 91-95
  • Peer Reviewed
• [Journal Article] Ovarian squamous cell carcinoma which metastasized 8 years after cervical conization for early microinvasive cervical cancer : a case report (2011)
  • Author(s): Hidaka T, Nakashima A, Hasegawa T, Nomoto K, Ishizawa S, Tsuneyama K, Takano Y and Shigeru S.
  • Journal Title: Jpn J Clin Oncol Volume: 41 Pages: 807-810
  • Peer Reviewed
• [Journal Article] Clinical implication of recent advances in our understanding of IL-17 and reproductive immunoloty (2011)
  • Author(s): Shigeru S, Nakashima A, Ito M and Shima T.
  • Journal Title: Expert Clin Immunol Volume: 7 Pages: 649-657
  • Peer Reviewed
• [Journal Article] Increased prevalence of peripheral blood granulysin-producing cytotoxic T lymphocytes in preeclampsia (2011)
  • Author(s): Molvarec A, Shiozaki A, Ito M, Toldi G, Stenczer B, Szarka A, Nakashima A, Vasarhelyi B, Rigo J, Shigeru S.
  • Journal Title: J Reprod Immunol Volume: 91 Pages: 56-63
  • Peer Reviewed
• [Journal Article] Anti-N-methyl-D-aspartate receptor encephalitis associated with ovarian immature teratoma (2011)
  • Author(s): Sameshima A, Hidaka T, Shima T, Nakashima A, Hasegawa T, Saito S.
  • Journal Title: J Obstet Gynaecol Res Volume: 37 Pages: 1883-6
  • Peer Reviewed
• [Journal Article] Stage I ovarian cancer cases during early, mid and late preganancy periods : Three case reports and review of the literature (2011)
  • Author(s): Hasegawa T, Ishii Y, Yonezawa R, Yoneda N, Shima T, Nakashima A, Hidaka T and Saito S.
  • Journal Title: J Obstet. Gynaecol. Res Volume: 37 Pages: 650-655
  • Peer Reviewed
• [Journal Article] A role for IL-17 in induction of an inflammation at the fetomaternal interface in preterm labour (2010)
  • Author(s): Ito M., Nakasima A., Hidaka T., Okabe M., Bac ND., Ina S., Yoneda S., Shiozaki A., Sumi S., Tuneyama K., Nikaido T., Saito S.
  • Journal Title: J Reprod Immunol Volume: 84 Pages: 75-85
  • Peer Reviewed
• [Journal Article] Cyclin A2 confers cisplatin resistance to endometrial carcinoma cells via up-regulation of an Akt-binding protein, periplakin (2010)
  • Author(s): Suzuki A., Horiuchi A., Ashida T., Miyamoto T., Kashima H., Nikaido T., Konishi I., Shiozawa T.
  • Journal Title: J Cell Mol Med Volume: 14 Pages: 2305-2317
  • Peer Reviewed
• [Journal Article] Circulating and decidual Th17 cell levels in healthy pregnancy (2010)
  • Author(s): Nakashima A., Ito M., Yoneda S., Shiozaki A., Hidaka T., Saito S.
  • Journal Title: Am J Reprod Immunol Volume: 63 Pages: 104-109
  • Peer Reviewed
• [Journal Article] Accumulation of IL-17-positive cells in decidua of inevitable abortion cases. (2010)
  • Author(s): Nakashima A., Ito M., Shima T., Bac N. D., Hidaka T., Saito S.
  • Journal Title: Am J Reprod Immunol. Am J Reprod Immunol Volume: 64 Pages: 4-11
  • Peer Reviewed
• [Journal Article] Systemic lymphadenectomy cannot be recommended for low-risk corpus cancer (2010)
  • Author(s): Hidaka T., Nakashima T., Shima T., Hasegawa T., Saito S.
  • Journal Title: Obstet Gynecol Int Volume: 2010 Pages: 1-5
  • Peer Reviewed
• [Journal Article] Bone metabolism after cessation of long-term hormone replacement therapy for postmenopausal osteoporosis (2010)
  • Author(s): Hasegawa T., Yonezawa R., Shima T., Tatematsu M., Nakashima A., Hidaka T., Saito S.
  • Journal Title: Osteoporosis Jpn Volume: 18 Pages: 133(481)-137(485)
  • Peer Reviewed
• [Journal Article] Endometrioid adenocarcinoma of the vagina with a microglandular pattern arising from endometriosis after hysterectomy (2010)
  • Author(s): Nomoto K., Hori T., Kiya C., Fukuoka J., Nakashima A., Hidaka T., Saito S., Mikami Y., Tsuneyama K., Takano Y.
  • Journal Title: Pathol Int Volume: 60 Pages: 636-641
  • Peer Reviewed
• [Journal Article] Stage I ovarian cancer cases during early, mid and late pregnancy periods : Three case reports and review of the literature (2010)
  • Author(s): Hasegawa T., Ishii Y., Yonezawa R., Yoneda N., Shima T., Nakashima A., Hidaka T., Saito S.
  • Journal Title: J Obstet Gynaecol Res
  • DOI: doi:10.1111/j.1447-0756.2010.01412.x.
  • Peer Reviewed
• [Journal Article] Carcinomatous meningitis associated with ovarian cancer complicated by SIADH (2010)
  • Author(s): Hasegawa T., Nakashima A., Ishimaru M., Shima T., Hidaka T., Saito S.
  • Journal Title: Jpn J Cancer Chemother Volume: 37 Pages: 739-742
  • Peer Reviewed
• [Journal Article] Regulatory T cells are necessary for implantation and maintenance during early stage of pregnancy, but not necessary during late stage of pregnancy in allogeneic mice (2010)
  • Author(s): Shima T., Sasaki Y., Itoh M., Nakashima A., Ishii N., Sugamura K., Saito S.
  • Journal Title: J. Reprod Immunol Volume: 85 Pages: 121-129
  • Peer Reviewed
• [Journal Article] Th1/Th2/Th17 and regulatory T cell paradigm in pregnancy (2010)
  • Author(s): Saito S., Nakashima A., Shima T., Ito M.
  • Journal Title: Am J Reprod Immunol Volume: 63 Pages: 601-610
  • Peer Reviewed
• [Journal Article] Immune surveillance during pregnancy (2010)
  • Author(s): Saito S., Shima T., Nakashima A., Lin Y.
  • Journal Title: Ind. J. Physiol. Pharmacol Volume: 54 Pages: 60-63
  • Peer Reviewed
• [Presentation] 子宮頸癌における癌幹細胞の細胞表面マーカー (2011)
  • Author(s): 吉田淑子,齋藤滋,二階堂敏雄
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 20111003-05
• [Presentation] 婦人科がん支持療法としての漢方治療 (2011)
  • Author(s): 日高隆雄
  • Organizer: 第63回日本産科婦人科学会学術講演会ランチョンセミナ
  • Place of Presentation: 大阪
  • Year and Date: 20110829-31
• [Presentation] 子宮頸部中腎腺癌(Mesonephric adenocarcinoma)の二例 (2011)
  • Author(s): 中島彰俊、飯沼ゆり子、稲田貢三子、島友子、長谷川徹、野本一博、日高隆雄、斎藤滋
  • Organizer: 第50回日本婦人科腫瘍学会学術講演会
  • Place of Presentation: 北海道札幌コンベンションセンター
  • Year and Date: 20110722-24
• [Presentation] 宮頸癌における癌幹細胞の細胞表面マーカの同定(意義) (2011)
  • Author(s): 吉田淑子,王芳,岡部素典,小池千加,周凱旋,齋藤滋,二階堂敏雄
  • Organizer: 第10回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 20110301-02
• [Presentation] 婦人科がん支持療法としての漢方治療 (2011)
  • Author(s): 日高隆雄
  • Organizer: 道東地区産婦人科医会学術講演会
  • Place of Presentation: 帯広
  • Year and Date: 2011-08-19
• [Presentation] Autophagy is an indispensable mechanism for extravillous trophoblast invasion (2010)
  • Author(s): Saito S., Nakashima A.
  • Organizer: 第69回日本癌学会学術総会シンポジウム"Cancer and autophagy"
  • Place of Presentation: 大阪
  • Year and Date: 20100922-24
• [Presentation] 子宮頚部中腎腺癌mesonephric adenocarsinomaの2例 (2010)
  • Author(s): 中島彰俊,飯沼ゆり子,佐藤幹奈,青木藍子,稲田貢三子,島友子,長谷川徹,日高隆雄,齋藤滋
  • Organizer: 第58回日本産科婦人科学会北日本連合地方部会総会・学術講演会
  • Place of Presentation: 金沢
  • Year and Date: 20100918-19
• [Presentation] The role of IL-17 in the pathogenesis of preterm labor (2010)
  • Author(s): Nakashima A., Ito M., Shima T., Yoneda S., Saito S.
  • Organizer: International symposium for immunology of reproduction, symposium "Infectious immunity"
  • Place of Presentation: Osaka
  • Year and Date: 20100828-29
• [Presentation] 口腔癌細胞株における癌幹細胞の同定 (2010)
  • Author(s): 能登善弘*,吉田淑子,小池千加,岡部素典,津野宏彰,野口誠,二階堂敏雄
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 20100318-19
• [Presentation] Identification of cervical cancer stem cells (2010)
  • Author(s): Fathy M., Koike C., Yoshida T., Okabe M., Nikaido T.
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 20100318-19
• [Presentation] 癌幹細胞の同定と解析 (2010)
  • Author(s): 二階堂敏雄
  • Organizer: 第6回河北省癌学会
  • Place of Presentation: 中国
  • Year and Date: 2010-07-10
• [Remarks]
  • URL: http://www.med.u-toyama.ac.jp/saiseiigaku/index.html