2011 Fiscal Year Final Research Report
New strategy for seasonal allergic rhinitis by exosomes
| Project/Area Number |
22659306
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | エクソゾーム / スギ花粉症 / MHC class II / CD9 / CD86 |
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| Research Abstract |
Exosomes are complex structures composed of a lipid bilayer that contains transmembrane proteins and encloses soluble hydrophilic components derived from the cytosol of the donor cells. They also contain the information of antigens with MHC class I & II. These molecules would be taken by neighboring cells and stimulate the receptors in the surface of cells. In this study we examined whether isolated exosomes from good responder of sublingual immunotherapy for allergic rhinitis(Japanese cedar pollen) could have an effect on immune systems. We got 15 to 80μg exosomes from the human sera. Western blots demonstrated that exosomes had MHC class IIαchain, CD9 and CD86 molecules. No band was found by anti-Cry J1(Japanese cedar pollen antigen) antibody in western blots. This study showed that we could isolate exosomes from human sera. However, we need more improvement methods to get high volume of exosomes and sensitivity to detect them for the advanced study.
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Research Products
(12 results)
