Development of Imaging Diagnosis for Lymphedema

2011 Fiscal Year Final Research Report

• Project/Area Number: 22659323
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Plastic surgery
• Research Institution: Okayama University
• Principal Investigator: NINOMIYA Yoshifumi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70126241)
• Co-Investigator(Kenkyū-buntansha): OOHASHI Toshitaka 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (50194262)
• Project Period (FY): 2010 – 2011
• Keywords: リンパ管 / リンパ浮腫 / リポソーム / 生体イメージング

Research Abstract

Current research aimed to establish a new active targeting liposome system for diagnosis of lymphedema, by targeting it to the lymphatic endothelial cell markers. Specific binding of the molecular targeting liposome to cultured lymphatic endothelial cells in vitro and visualization of lymphatic vessels in normal mice were demonstrated. However, we could not establish the measuring system in rat lymphedema model. As for its related technique, we could develop a new liposome encapsulated with colloidal gold which enables both light-and electron-microscopic analysis.

Research Products

• [Journal Article] Bral2 is indispensable for the proper localization of brevican and the structural integrity of the perineuronal net in the brain stem and cerebellum (2011)
  • Author(s): Bekku Y, Saito M, Moser M, Fuchigami M, Maehara A, Nakayama M, Kusachi S, Ninomiya Y, Oohashi T
  • Journal Title: J Comp Neurol
  • DOI: doi:10.1002/cne.23009
  • Peer Reviewed
• [Journal Article] Development of an active targeting liposome encapsulated with high-density colloidal gold for transmission electron microscopy (2011)
  • Author(s): Minematsu H, Otani T, Oohashi T, Hirai M, Oie K, Igarashi K, Ohtsuka A
  • Journal Title: J Electron Microsc Volume: 60(1) Pages: 95-99
  • Peer Reviewed
• [Journal Article] Clonal overgrowth of esophageal smooth muscle cells in diffuse leiomyomatosis-Alport syndrome caused by partial deletion in COL4A5 and COL4A6 genes (2011)
  • Author(s): Oohashi T, Naito I, Ueki Y, Yamatsuji T, Permpoon R, Tanaka N, Naomoto Y, Ninomiya Y
  • Journal Title: Matrix Biol Volume: 30(1) Pages: 3-8
  • Peer Reviewed
• [Journal Article] 関節軟骨のバイオイメージング (2011)
  • Author(s): 大橋俊孝, 西田圭一郎
  • Journal Title: Clinical Calcium Volume: 21(6) Pages: 100-106
• [Journal Article] Bral : its role in diffusion barrier formation and conduction velocity in theCNS (2010)
  • Author(s): Bekku Y, Vargova L, Goto Y, Vorisek I, Dmytrenko L, Narasaki M, Ohtsuka A, Fassler R, Ninomiya Y, Sykova E, Oohashi T
  • Journal Title: J Neurosci Volume: 30(8) Pages: 3113-23
  • Peer Reviewed
• [Journal Article] 軟骨組織のバイオイメージング (2010)
  • Author(s): 大橋俊孝
  • Journal Title: 臨床整形外科 Volume: 45(5) Pages: 454-457
• [Presentation] 炎症標的化金コロイド内包リポソームのリウマチ関節炎症部位への集積 (2012)
  • Author(s): 前原亜美
  • Organizer: 第25回日本軟骨代謝学会
  • Place of Presentation: 名古屋
  • Year and Date: 20120309-10
• [Presentation] 炎症標的化金コロイド内包リポソームのリウマチ関節炎症部位への集積 (2011)
  • Author(s): 古谷満寿美
  • Organizer: 第43回日本結合組織学会,第58回マトリックス研究会
  • Place of Presentation: 大分
  • Year and Date: 20110610-11
• [Presentation] 炎症標的化リポソームのリウマチ関節炎症部位への集積 (2010)
  • Author(s): 大橋俊孝
  • Organizer: 病理学会カンファレンス-電子顕微鏡観察-
  • Place of Presentation: 岡山
  • Year and Date: 20100806-07
• [Presentation] シアリルルイスX糖鎖結合リポソームのリウマチ関節炎症部位への集積についての電子顕微鏡的検討 (2010)
  • Author(s): 松本衣未
  • Organizer: 第51回日本生化学会中国.四国支部例会
  • Place of Presentation: 山口
  • Year and Date: 20100514-15
• [Remarks]
  • URL: http://mbb-okayama.sakura.ne.jp/