Functional relation of stress response in blood cells and brain after hypoxia

2012 Fiscal Year Final Research Report

• Project/Area Number: 22659325
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Emergency medicine
• Research Institution: Yamagata University
• Principal Investigator: GOTO Kaoru 山形大学, 医学部, 教授 (30234975)
• Co-Investigator(Kenkyū-buntansha): ISEKI Ken 山形大学, 医学部, 准教授 (70332921)
• Project Period (FY): 2010 – 2012
• Keywords: 低酸素脳症 / 生体ストレス / 脂質代謝酵素

Research Abstract

Biological stress determines recovery from emergency conditions, such as accidents and surgery and affects intracellular signal transduction. Diacylglycerol kinase (DGK) is an enzyme that metabolizes the second messenger DG in phosphoinositide turnover. Recent studies have indicated an involvement of diacylglycerol kinase (DGK) in various diseases such as cancer, diabetes, inflammation and stroke. In the present study we performed an experiment at the organismal level using hypoxic chamber stress (oxygen concentration 5%). After 3 days of transient hypoxic stress, we found that DGKζ was translocated from the nucleus to the cytoplasm. These results provide an evidence that nucleo-cytoplasmic translocation of DGKζ may be an index of early phase of apoptosis.

Research Products

• [Journal Article] The emerging multiple roles of nuclear Akt (2012)
  • Author(s): Martelli AM,Tabellini G, Bressanin D, Ognibene A, Goto K, Cocco L, Evangelisti C
  • Journal Title: Biochim Biophys Acta Volume: 1823 Pages: 2168-78
  • DOI: doi:10.1016/j.bbamcr.2012.08.017.
  • Peer Reviewed
• [Journal Article] DGKζ is degraded through the cytoplasmic ubiquitin-proteasome system under excitotoxic conditions, which causes neuronal apoptosis because of aberrant cell cycle reentry (2012)
  • Author(s): Okada M, Hozumi Y, Tanaka T, Suzuki Y, Yanagida M, Araki Y, Evangelisti C, Yagisawa H, Topham MK, Martelli AM, Goto K
  • Journal Title: Cell Signal Volume: 24 Pages: 1573-82
  • DOI: doi:10.1016/j.cellsig.2012.03.021.
  • Peer Reviewed
• [Journal Article] DGKζ is involved in LPS-activated phagocytosis through IQGAP1/Rac1 pathway (2012)
  • Author(s): Okada M, Hozumi Y, Iwazaki K, Misaki K, Yanagida M, Araki Y, Watanabe T, Yagisawa H, Topham MK, Kaibuchi K, Goto K
  • Journal Title: Biochem Biophys Res Commun Volume: 420 Pages: 479-84
  • DOI: doi:10.1016/j.bbrc.2012.03.057.
  • Peer Reviewed
• [Journal Article] NMDA receptor-mediated Ca(2+) influx triggers nucleocytoplasmic translocation of diacylglycerol kinase ζ under oxygen-glucose deprivation conditions, an in vitro model of ischemia,in rat hippocampal slices (2012)
  • Author(s): Suzuki Y, Yamazaki Y, Hozumi Y, Okada M, Tanaka T, Iseki K, Ohta N, Aoyagi M, Fujii S, Goto K
  • Journal Title: Histochem Cell Biol Volume: 137 Pages: 499-511
  • DOI: doi:10.1007/s00418-011-0907-y.
  • Peer Reviewed
• [Journal Article] Altered expression of diacylglycerol kinase isozymes in regenerating liver (2012)
  • Author(s): Nakano T, Hozumi Y, Iwazaki K, Okumoto K, Iseki K, Saito T, Kawata S, Wakabayashi I, Goto K
  • Journal Title: J Histochem Cytochem Volume: 60 Pages: 130-8
  • DOI: doi:10.1369/0022155411429154.
  • Peer Reviewed
• [Journal Article] NAP Interaction of nucleosome assembly proteins abolishes nuclear localization of DGKζ by attenuating its association with importins (2011)
  • Author(s): Okada M, Hozumi Y, Ichimura T, Tanaka T, Hasegawa H, Yamamoto M, Takahashi N, Iseki K, Yagisawa H, Shinkawa T, Isobe T, Goto K
  • Journal Title: Exp Cell Res Volume: 317 Pages: 2853-63
  • DOI: doi:10.1016/j.yexcr.2011.09.014
  • Peer Reviewed
• [Journal Article] Excitotoxicity by kainate-induced seizure causes diacylglycerol kinase ζ to shuttle from the nucleus to the cytoplasm in hippocampal neurons (2011)
  • Author(s): Saino-Saito S, Hozumi Y, Goto K
  • Journal Title: Neurosci Lett Volume: 494 Pages: 185-9
  • DOI: doi:10.1016/j.neulet.2011.02.062.
  • Peer Reviewed
• [Journal Article] Nuclear relocation of DGKζ in cardiomyocytes under conditions of ischemia/reperfusion (2011)
  • Author(s): Akiyama H, Hozumi Y, Nakano T, Kubota I, Goto K
  • Journal Title: Histol Histophathol Volume: 26 Pages: 1383-1390
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21938675
  • Peer Reviewed
• [Journal Article] Nuclear phosphoinositides and their roles in cell biology and disease (2011)
  • Author(s): Martelli AM, Ognibene A, Buontempo F, Fini M, Bressanin D, Goto K, McCubrey JA, Cocco L, Evangelisti C
  • Journal Title: Critical Reviews in Biochemistry and Molecular Biology Volume: 46 Pages: 436-57
  • DOI: doi:10.3109/10409238.2011.609530
  • Peer Reviewed
• [Journal Article] Identification of a functional nuclear export sequence in diacylglycerol kinase-zeta (2010)
  • Author(s): Evangelisti C, Gaboardi GC, Billi AM, Ognibene A, Goto K, Tazzari PL, McCubrey JA, Martelli AM
  • Journal Title: Cell Cycle Volume: 9 Pages: 1-6
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20023381
  • Peer Reviewed
• [Presentation] Expression and localization of diacylglycerol kinase in rat retina (2012)
  • Author(s): Hozumi Y, Goto K
  • Organizer: 42th Annual Meeting of Neuroscience, Convention Center
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20121013-17
• [Presentation] Cytoplasmic localization of diacylglycerol kinase zeta suppresses p53 induction after DNA damage (2012)
  • Author(s): Tanaka T, Goto K
  • Organizer: 42th Annual Meeting of Neuroscience, Convention Center
  • Place of Presentation: New Orleans, USA
  • Year and Date: 20121013-17
• [Presentation] Functional implication of diacylglycerol kinase in stress response (2012)
  • Author(s): 後藤薫
  • Organizer: 第89回日本生理学会総会
  • Place of Presentation: 松本
  • Year and Date: 20120329-31
• [Presentation] 生体ストレスにおける脂質性二次伝達物質代謝酵素ジアシルグリセロールキナーゼ(DGK)の応答 (2012)
  • Author(s): 後藤薫
  • Organizer: 第117回日本解剖学会総会
  • Place of Presentation: 甲府
  • Year and Date: 20120326-28
• [Presentation] イプシロン型ジアシルグリセロールキナーゼの小脳における発現局在解析 (2012)
  • Author(s): 八月朔日泰和、後藤薫
  • Organizer: 第117回日本解剖学会総会
  • Place of Presentation: 甲府
  • Year and Date: 20120326-28
• [Presentation] DGKζ 結合タンパクNAP はDGKζ を細胞質へ移行させ細胞死を抑制する (2012)
  • Author(s): 田中俊昭、岡田雅司、後藤薫
  • Organizer: 第117回日本解剖学会総会
  • Place of Presentation: 甲府
  • Year and Date: 20120326-28
• [Presentation] 遺伝子導入培養細胞を用いたDGKεの細胞内局在解析 (2012)
  • Author(s): 松井祐興、後藤薫
  • Organizer: 第117回日本解剖学会総会
  • Place of Presentation: 甲府
  • Year and Date: 20120326-28
• [Presentation] DGKζと Retinoblastoma 蛋白の軟骨細胞分化調節における役割 (2012)
  • Author(s): 土谷理恵子、飯野光喜、後藤薫
  • Organizer: 第117回 日本解剖学会総会
  • Place of Presentation: 甲府
  • Year and Date: 20120326-28
• [Presentation] Functional significance of diacylglycerol kinase in stress response (2011)
  • Author(s): Goto K
  • Organizer: The 116th Annual Meeting of The Japanese Association of Anatomists and the 88th Annual Meeting of The Physiological Society of Japan
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20110328-30
• [Presentation] Functional implications of diacylglycerol kinase β on the perisynaptic site in developing neurons (2011)
  • Author(s): Hozumi Y, Goto K
  • Organizer: The 116th Annual Meeting of The Japanese Association of Anatomists and the 88th Annual Meeting of The Physiological Society of Japan
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20110328-30
• [Presentation] DGKζ is a suppressor of excitotoxic neuronal cell death (2011)
  • Author(s): Okada M, Goto K
  • Organizer: The 116th Annual Meeting of The Japanese Association of Anatomists and the 88th Annual Meeting of The Physiological Society of Japan
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20110328-30
• [Presentation] Cytoplasmic Diacylglycerol kinase zeta suppresses p53 stability and apoptosis (2011)
  • Author(s): Tanaka T. Goto K
  • Organizer: The 116th Annual Meeting of The Japanese Association of Anatomists and the 88th Annual Meeting of The Physiological Society of Japan
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20110328-30
• [Presentation] Cytoplasmic diacylglycerol kinase ζ suppresses p53 stability and apoptosis (2011)
  • Author(s): Tanaka T, Goto K
  • Organizer: Gordon Conference
  • Place of Presentation: Ventura, CA, USA
  • Year and Date: 20110227-0304