Investigation for development of technique reprogramming of mesenchymal stem cells using epigenetic analyses

2011 Fiscal Year Final Research Report

• Project/Area Number: 22659336
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Functional basic dentistry
• Research Institution: Osaka University
• Principal Investigator: NISHIMURA Riko 大阪大学, 大学院・歯学研究科, 准教授 (60294112)
• Co-Investigator(Renkei-kenkyūsha): YONEDA Toshiyuki 大阪大学, 大学院・歯学研究科, 教授 (80142313) ; HATA Kenji 大阪大学, 大学院・歯学研究科, 講師 (80444496)
• Project Period (FY): 2010 – 2011
• Keywords: エピジェネティクス / 転写 / 未分化間葉系幹細胞 / 骨芽細胞 / 軟骨細胞

Research Abstract

Mesenchymal stem cells(MSCs) have an unique ability to differentiate into several different cell linage including osteoblasts, chondrocytes, adipocytes and myocytes. In contrast, most of fibroblasts lack the ability. We focused on this difference between MSCs and fibroblasts, and examined differences of expression profiles and epigenetic information, in order to understand the molecules that are necessary for multipotent activity of MSCs. In addition, we attempted to approach the reprogramming of MSCs.

Research Products

• [Journal Article] 内軟骨性骨形成過程における転写因子の役割と機能制御 (2012)
  • Author(s): 西村理行、波多賢二、高島利加子、吉田倫子
  • Journal Title: Clinical Calcium Volume: 22 Pages: 643-652
• [Journal Article] Regulation of bone and cartilage development by network between BMP signaling and transcription factors (2012)
  • Author(s): Nishimura R, Hata K, Mastubara T, Wakabayashi M, Yoneda T
  • Journal Title: J Biochem Volume: 151 Pages: 247-254
  • Peer Reviewed
• [Journal Article] Regulation of endochondral ossification by transcription factors (2012)
  • Author(s): Nishimura R, Hata K, Ono K, Amano K, Takigawa Y, Wakabayashi M, Takashima R, Yoneda T
  • Journal Title: Frontiers in Bioscience Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Distinct mechanisms are responsible for osteopenia and growth retardation in OASIS-deficient mice (2011)
  • Author(s): Murakami T, Hino S, Nishimura R, Yoneda T, Wanaka A, Imaizumi K
  • Journal Title: Bone Volume: 48 Pages: 514-523
  • Peer Reviewed
• [Journal Article] Arid5a cooperates with Sox9 to stimulate chondrocyte-specific transcription (2011)
  • Author(s): Amano K, Hata K, Muramatsu S, Wakabayashi M, Takigawa Y, Ono K, Nakanishi M, Takashima R, Kogo M, Matsuda A, Nishimura R, Yoneda T
  • Journal Title: Mol Biol Cell Volume: 22 Pages: 1300-1311
  • Peer Reviewed
• [Journal Article] 骨芽細胞の分化制御機構 (2011)
  • Author(s): 西村理行
  • Journal Title: Clinical Calcium Volume: 21 Pages: 103-112
• [Journal Article] Carbonic anhydrase II regulates differentiation of ameloblasts via Intracellular pH-dependent JNK signaling pathway (2010)
  • Author(s): Wang X, Suzawa T, Ohtsuka H, Zhao B, Miyamoto Y, Miyauchi T, Nishimura R, Inoue T, Nakamura M, Baba K, Kamijo R
  • Journal Title: J Cell Physiol Volume: 225 Pages: 709-719
  • Peer Reviewed
• [Journal Article] Cbp recruitment of Csk into lipid rafts is critical to c-Src kinase activity and bone resorption in osteoclasts (2010)
  • Author(s): Matsubara T, Ikeda F, Hata K, Nakanishi M, Okada M, Yasuda H, Nishimura R, Yoneda T
  • Journal Title: J Bone Miner Res Volume: 25 Pages: 1068-1076
  • Peer Reviewed
• [Journal Article] A transcription factor Znf219 regulates chondrocyte differentiation by assembling transcription factory with Sox9 (2010)
  • Author(s): Takigawa Y, Hata K, Muramatsu S, Amano K, Ono K, Wakabayashi M, Matsuda A, Takada K, Nishimura R, Yoneda T
  • Journal Title: J Cell Sci Volume: 123 Pages: 3780-3788
  • Peer Reviewed
• [Journal Article] オステオポンチン、オステオネクチン.血液・尿化学検査 (2010)
  • Author(s): 西村理行
  • Journal Title: 日本臨牀増刊号 Pages: 233-235
• [Presentation] The transcription factor Arid5B is a novel partner of Sox9 in endochondral bone formation (2011)
  • Author(s): Takashima R, Hata K, Wakabayashi M, Nakanishi M, Ono K, Amano K, Whitson R, Maeda Y, Nishimura R, Yoneda T
  • Organizer: American Society for Bone and Mineral Research 2011 Annual Meeting
  • Place of Presentation: San Diego Convention Center
  • Year and Date: 20110916-20
• [Presentation] The transcription factor Arid5b modulates endochondral bone formation in cooperation with Sox9 (2011)
  • Author(s): Takashima R, Hata K, Wakabayashi M, Nakanishi M, Ono K, Amano K, Whitson R, Maeda Y, Nishimura R, Yoneda T
  • Organizer: Joint 2nd Asia-Pacific Osteoporosis and Bone Meeting & ANZBMS Annual Meeting
  • Place of Presentation: Australia, Gold Coast
  • Year and Date: 20110904-08
• [Presentation] Arid5bはSox9の転写機能を促進することにより軟骨細胞分化を制御する (2011)
  • Author(s): 高島理加子、波多賢二、若林真、小野孝一郎、天野克比古、中西雅子、前田芳信、西村理行、米田俊之
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 20110728-30
• [Presentation] Regulation of bone development by Sox9 and Osterix (2011)
  • Author(s): Nishimura R
  • Organizer: 7^<th> Bone Biology Forum
  • Place of Presentation: Fuji Institute of Education and Training
  • Year and Date: 2011-08-20
• [Presentation] 骨格形成過程における転写因子の制御機構 (2011)
  • Author(s): 西村理行
  • Organizer: 第84回日本内分泌学会学術総会
  • Place of Presentation: 神戸国際会議場・神戸国際展示場
  • Year and Date: 2011-04-22
• [Presentation] 骨形成過程における転写因子調節機構 (2010)
  • Author(s): 西村理行
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 京王プラザホテル
  • Year and Date: 2010-07-23