2011 Fiscal Year Final Research Report
Induction of Jaw Bone and Tooth Germ from murine embryonic stem cells and human bone marrow stem cells in serum-free defined suspension culture
| Project/Area Number |
22659369
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
OKAMOTO Tetsuji 広島大学, 大学院・医歯薬学総合研究科, 教授 (00169153)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUI Yasuto 広島大学, 大学院・医歯薬学総合研究科, 助教 (90363085)
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| Project Period (FY) |
2010 – 2011
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| Keywords | マウスES細胞 / 骨髄幹細胞 / マウスiPS細胞 / アクチビン / 軟骨誘導 / 無血清培養 |
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| Research Abstract |
Human Embryonic Stem(hES) cells and human induced Pluripotent Stem(hiPS) cells are commonly maintained on inactivated mouse embryonic fibroblast as feeder cells in medium supplemented with FBS or proprietary replacements. Use of culture media containing undefined or unknown components has limited the development of applications for pluripotent cells because of the relative lack of knowledge regarding cell responses to differentiating growth factors. Therefore we developed a serum-free medium, designated hESF9, in which human ES cells can be maintained in undifferentiated state without feeder cells. We have developed a serum-free defined medium for culturing hiPS cells, which can maintain proliferation, self-renewal and pluripotency. As this simple serum-free adherent monoculture system will allow us to elucidate the cell responses to growth factors under defined conditions, and can eliminate the risk might be brought by undefined pathogens.
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