2013 Fiscal Year Final Research Report
Studies on mechanosensing mechanisms in reproductive cells and embryonic developments applied with mechanical stimuli
| Project/Area Number |
22680036
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Okayama University |
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Principal Investigator |
KOJI Matsuura 岡山大学, 医歯(薬)学総合研究科, 講師 (70443223)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 精子 / 受精卵 / メカニカルストレス / 細胞内カルシウム濃度 / マイクロ流路 / 共焦点蛍光顕微鏡 |
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| Research Abstract |
To elucidate sensing mechanisms to mechanical stimuli in mammalian sperm and embryo, our group developed microfluidic devices to mimic mechanical stimuli in the oviduct and exhibited microscopic observation of human sperm and mouse embryos which were mechanically stimulated. Intracellular calcium concentration in the fixed sperm head increased after applying fluid shear stress of 10 dyn/cm2. Furthermore, direction of the motile sperms is strongly affected by the shear stress distribution in the microfluids. We have found thresholds toward detrimental shear stress to mouse embryo for pre-implantation embryonic development.
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