2013 Fiscal Year Final Research Report
Development of a novel active targeting system based on split SNAP-tag
| Project/Area Number |
22680041
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
MIE Masayasu 東京工業大学, 総合理工学研究科(研究院), 准教授 (40334528)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Keywords | SNAP-tag / complementation / タンパク質間相互作用 / ターゲティング |
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| Research Abstract |
In this experiment, a novel active targeting system based on split SNAP-tag was developed. Firstly, split SNAP-tag complementation was studied. Split SNAP-tags, fragments of divided SNAP-tag were fused to proteins that can interact with each other. After incubation with a fluorescent SNAP-tag substrate, cells that expressed split SNAP-tag fusion proteins generated fluorescent signals when these proteins interacted. It was shown that split SNAP-tag labeling method should be a useful tool for visualization of protein-protein interaction processes.
Next, for using DNA aptamer in our system, we developed a method for site-specific labeling of single-stranded DNA (ssDNA) to a recombinant protein of interest (POI) through the Gene-A* protein (Gene-A*) from bacteriophage phi X174, without any chemical modifications of ssDNA.
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