Functionalized conducting polymer biointerfaces for cell engineering: specific adhesion of proteins and cells, cell patterning, and dynamic binding/releasing by electrical stimuli.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22681016
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: YU Hsiao-hua 独立行政法人理化学研究所, Yu 独立主幹研究ユニット, ユニットリーダー(独立主幹研究員) (70529908)
• Research Collaborator: TSENG Hsian-rong University of California, Los Angeles, Department of Molecular and Medical Pharmacology, Professor,
• Project Period (FY): 2010 – 2012
• Keywords: 有機化学 / 導電性高分子 / 有機導体 / バイオテクノロジー / バイオインターフェイス / ナノバイオ / ナノ材料

Research Abstract

During the 3-year of this original project, we have successfully achieved all the original goals and build a new concept to utilize molecularly designed conducting polymer to control cellular behavior on the interface between cells and materials. The interface is also dynamic therefore we can control the interface by both chemical and electrical stimulation. The major achievements include: (1) Successfully build a monomer library consists of more than 20 new thiophene based monomers. (2) Control the cellular interaction with the materials with introduction of the monomers that prohibit proteins and cells binding to the materials. (3) Switch the surface properties from layered polythiophenes assemblies. (4) Cell patterning with top-down fabrication of bottom-up electropolymerization assemblies. (5) Dynamic polythiophene-cell interface with electrical stimulation. We further studied the effect of chemical and electrical stimulation on the cell engineering with these materials. The major results include: (1) Conducting polymer nano-networks for cell engineering. Thiophene monomer with a variety of functional groups would form EDOT nano-networks with the nanofiber dimensions between 20 to 40 nm by controlling the electropolymerization kinetics. (2) Enhanced cell-capturing on the nanostructured conducting polymer with capture agents. More efficient capturing of cancer cells were demonstrated on antibody conjugated polythiophene nanodots.

Research Products

• [Journal Article] (2013)
  • Author(s): Hou, S.; Zhao, H.; Zhao, L.; Shen, Q.; Wei, K. S.; Suh D. Y.; Nakao, A.; Xiong B.; Luo, S.-C.; Tseng, H.-R.; Yu, Hsiao-hua
  • Journal Title: Adv. Mater. Volume: 25 Pages: 1547-1551
  • Peer Reviewed
• [Journal Article] (2012)
  • Author(s): Zhao, H.; Liu, C.-H.; Luo, S.-C.; Zhu, B.; Wang, T.-H.; Hsu, H.-F.; Yu, Hsiao-hua
  • Journal Title: Macromolecules Volume: 45 Pages: 7783-7790
  • Peer Reviewed
• [Journal Article] (2012)
  • Author(s): Luo, S.-C.; Zhu, B.; Kantchev, E. A. B.; Siang, Y. W.; Yu, Hsiao-hua
  • Journal Title: Chem. Commun. Volume: 48 Pages: 6942-6944
  • Peer Reviewed
• [Journal Article] (2012)
  • Author(s): Lacher, S.; Obata, N.; Luo, S.-C.; Matsuo, Y.; Zhu, B.; Yu, Hsiao-hua; Nakamura, E
  • Journal Title: ACS Appl. Mater. Interfaces Volume: 4 Pages: 3396-3404
  • Peer Reviewed
• [Journal Article] (2012)
  • Author(s): Luo, S.-C.; Sekine, J.; Zhu, B.; Zhao, H.; Nakao, A.; Yu, Hsiao-hua
  • Journal Title: ACS Nano Volume: 6 Pages: 3018-3026
  • Peer Reviewed
• [Journal Article] (2012)
  • Author(s): Zhao, H.; Zhu, B.; Sekine, J.; Luo, S.-C.; Yu, Hsiao-hua
  • Journal Title: ACS Appl. Mater. Interfaces Volume: 4 Pages: 680-686
  • Peer Reviewed
• [Journal Article] (2012)
  • Author(s): Guo, Z.; Hu, L.; Yu, Hsiao-hua; Can, X.; Gu, H.
  • Journal Title: RSC Advances Volume: 2 Pages: 3477-3480
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Pardilla, J. A.; Otero, T. F.; Yu, Hsiao-hua
  • Journal Title: Electrochimica Acta Volume: 56 Pages: 10238-10245
• [Journal Article] (2011)
  • Author(s): Sekine, J.; Luo, S.-C.; Wang, S.; Zhu, B.; Tseng, H.R.; Yu, Hsiao-hua
  • Journal Title: Adv. Mater Volume: 23 Pages: 4788-4792
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Liu, C.-Y.; Zhao, H.; Yu, Hsiao-hua
  • Journal Title: Org. Lett. Volume: 13 Pages: 4068-4071
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Luo, S.-C.; Zhu, B.; Nakao, A.; Nakatomi, R.; Yu, Hsiao-hua
  • Journal Title: Adv. Eng. Mater. (Advanced Biomaterials) Volume: 13 Pages: B423-B427
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Tansil, N. C.; Kantchev, E. A. B.; Gao, Z.; Yu, Hsiao-hua
  • Journal Title: Chem. Commun. Volume: 47 Pages: 1533-1535
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Luo, S.-C.; Liour, S. S.; Yu, Hsiao-hua
  • Journal Title: Chem. Commun. Volume: 46 Pages: 4731-4733
  • Peer Reviewed
• [Journal Article]
  • Author(s): Zhao, H.; Zhu, B.; Luo, S.-C.; Lin, H.-A.; Nakao, A.; Yamashita, Y.; Yu, Hsiao-hua
  • Journal Title: ACS Appl. Mater. Interfaces Volume: (referred)
  • DOI: DOI:10.1021/am400135c.
  • Peer Reviewed
• [Journal Article]
  • Author(s): Lin, H.-A.; Luo, S.-C.; Zhu, B.; Yamashita, Y.; Yu, Hsiao-hua
  • Journal Title: Adv. Funct. Mater. Volume: 25(referred)
  • DOI: DOI:10.1002/adfm.201203006.
  • Peer Reviewed
• [Presentation] Conducting polymer as biomaterials: control the cell-materials interfaces (2011)
  • Author(s): Hsiao-hua Yu
  • Organizer: China-Japan-Korea Foresight Joint Symposium
  • Place of Presentation: Guilin, China
  • Year and Date: 2011-05-29
• [Presentation] Organic conductive biomaterials: from efficient syntheses to bioengineering applications (2011)
  • Author(s): Hsiao-hua Yu
  • Organizer: Intelligent Polymer Research Institute, University of Wollongong
  • Place of Presentation: Wollongong, Australia
  • Year and Date: 2011-05-17
• [Presentation] Biomimetic conductive membranes produced via inverse microemulsionelectron-copolymerization as neural interfaces (2011)
  • Author(s): Bo Zhu, Shyh-Chyang Luo, Jun Sekine, Hsiao-hua Yu
  • Organizer: E-MRS ICAM IUMRS 2011 Spring Meeting
  • Place of Presentation: Nice, France
  • Year and Date: 2011-05-11
• [Presentation] Conducting polymer biointerface for biosensing and cell engineering (2010)
  • Author(s): Hsiao-hua Yu
  • Organizer: IEEE Nano 2010 and Nano Korea 2010, TS6 "Nano-Sensors and Nano Membranes"
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2010-08-18
• [Presentation] Controlling cell and biomacromolecule adhesion on conducting polymer interface (2010)
  • Author(s): Hsiao-hua Yu
  • Organizer: E-MRS 2010 Spring Meeting, Symposium B "Functional Bioniterfaces
  • Place of Presentation: Strasbourg, France
  • Year and Date: 2010-06-08