2013 Fiscal Year Final Research Report
Multi-omics study to elucidate the molecular mechanism of anhydrobiosis in tardigrade Ramazzottius varieornatus
| Project/Area Number |
22681029
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
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| Research Institution | Keio University |
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Principal Investigator |
ARAKAWA Kazuharu 慶應義塾大学, 政策・メディア研究科, 特任講師 (40453550)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | メタボローム / システム生物学 / プロテオーム / 乾眠 / クマムシ / クリプトビオシス / マルチオミクス / バイオインフォマティクス |
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| Research Abstract |
Limno-terrestrial tardigrades can withstand extreme environments and almost complete desiccation through a mechanism called anhydrobiosis, but the its molecular mechanism is yet to be explored. To this end, we have conducted a comprehensive multi-omics analysis using the tardigrade Ramazzottius varieornatus, which is a potential model species for anhydrobiosis. In order to analyze the metabolic changes in the active and dehydrated states, we measured the metabolome in both conditions using two types of high-throughput mass spectrometry (MS) systems, CE-TOFMS and LC-TOFMS. Moreover, phospho-proteome was also analyzed to observed the metabolic regulations. As a result, while changes in gene expression profiles are limited in between active and tun states, dynamic changes were observed in the metabolism of this species in response to desiccation. Changes in the metabolic profiles suggests complex intracellular responses to oxidative and osmotic stress.
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[Journal Article] Analysis of DNA Repair and Protection in the Tardigrade Ramazzottius varieornatus and Hypsibius dujardini after Exposure to UVC Radiation2013
Author(s)
Daiki D. Horikawa, John Cumbers, Iori Sakakibara, Dana Rogoff, Stefan Leuko, Raechel Harnoto, Kazuharu Arakawa, Toshiaki Katayama, Takekazu Kunieda, Atsushi Toyoda, Asao Fujiyama, Lynn J. Rothschild
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Journal Title
PLoS One
Volume: Vol.8
Pages: e6479
Peer Reviewed
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[Journal Article] Two families of novel abundant heat-soluble proteins in an anhydrobiotic tardigrade2012
Author(s)
Ayami Yamaguchi, Sae Tanaka, Shiho Yamaguchi, Hirokazu Kuwahara, Chizuko Takamura, Shinobu Ohmi, Daiki D. Horikawa, Atsushi Toyoda, Toshiaki Katayama, Kazuharu Arakawa, Asao Fujiyama, Takeo Kubo, Takekazu Kunieda
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Journal Title
PLoS One
Volume: Vol.7
Pages: e44209
Peer Reviewed
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