2012 Fiscal Year Final Research Report
Development of anti-cancer agents targeting the endocytic pathway
| Project/Area Number |
22681034
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KAWATANI Makoto 独立行政法人理化学研究所, 化合物ライブラリー評価研究チーム, 専任研究員 (50391925)
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| Research Collaborator |
MUROI Makoto 独立行政法人理化学研究所, 化合物ライブラリー評価研究チーム, 専任研究員 (30261168)
FUTAMURA Yushi 独立行政法人理化学研究所, 化学情報・化合物創製チーム, 協力研究員 (70525857)
KAZAMI Sayaka 独立行政法人理化学研究所, 化合物ライブラリー評価研究チーム, 協力技術員
HONDA Kaori 独立行政法人理化学研究所, 化合物ライブラリー評価研究チーム, 協力技術員
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| Project Period (FY) |
2010 – 2012
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| Keywords | 癌 / 小分子化合物 / エンドサイトーシス |
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| Research Abstract |
The purpose of this study is to construct a cell morphology-based high-content screening system and to develop anti-cancer agents targeting the endocytic pathway. We constructed a chemical-genetic phenotype profiling system based on the high-content cell morphology database, Morphobase. We showed that NPD1801, which perturbs the endocytic pathway, targeted calmodulin, caused the activation of Ras-Raf pathway, the inhibition of the fusion of late endosome and lysosome, and the dysfunction of lysosome, and resulted in the cancer cell death.
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[Journal Article] Morphobase, an encyclopedic cell morphology database, and its use for drug target identification.2012
Author(s)
Futamura Y., Kawatani M., Kazami S., Tanaka K., Muroi M., Shimizu T., Tomita K., Watanabe N., and Osada H.
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Journal Title
Chem. Biol.
Volume: 19
Pages: 1620-1630
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[Journal Article] Violaceols function as actin inhibitors inducing cell shape elongation in fibroblast cells.2012
Author(s)
Asami Y., Jang J.-H., Oh H., Sohn J. H., Kim J. W., Moon D. O., Kwon O., Kawatani M., Osada H., Kim B. Y., and Ahn J. S.
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Journal Title
Biosci. Biotechnol. Biochem.
Volume: 76
Pages: 1431-1437
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[Journal Article] Effect of reveromycin A on experimental tooth movement in OPG-/- mice.2012
Author(s)
Tanaka M., Miyazawa K., Tabuchi M., Yabumoto T., Kadota M., Yoshizako M., Yamane C., Kawatani M., Osada H., Maeda H., and Goto S.
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Journal Title
J. Dent. Res.
Volume: 91
Pages: 771-776
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[Journal Article] Genetic safeguard against mycotoxin cyclopiazonic acid production in Aspergillus oryzae.2011
Author(s)
Kato N., Tokuoka M., Shinohara Y., Kawatani M., Uramoto M., Seshime Y., Fujii I., Kitamoto K., Takahashi T., Takahashi S., Koyama Y., and Osada, H.
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Journal Title
Chembiochem
Volume: 12
Pages: 1376-1382
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[Journal Article] Glyoxalase-I is a novel target against Bcr-Abl+ leukemic cells acquiring stem-like characteristics in hypoxic environment.2010
Author(s)
Takeuchi M., Kimura S., Kuroda J., Ashihara E., Kawatani M., Osada H., Umezawa K., Yasui E., Imoto M., Tsuruo T., Yokota A., Tanaka R., Nagao R., Nakahata T., Fujiyama Y., and Maekawa T.
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Journal Title
Cell Death Diff.
Volume: 17
Pages: 1211-1220
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