Elucidation of the fate of noroviruses in water environment by means of polysaccharides from enteric bacteria

2013 Fiscal Year Final Research Report

• Project/Area Number: 22686049
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Civil and environmental engineering
• Research Institution: Hokkaido University
• Principal Investigator: SANO DAISUKE 北海道大学, 工学(系)研究科(研究院), 准教授 (80550368)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Keywords: ノロウイルス / 腸内細菌 / 水環境中動態 / 吸着

Research Abstract

Human norovirus is one of the leading causes of infectious gastroenteritis worldwide. Infections of human noroviruses are not controllable, and there are no effective prevention measures. In this study, I hypothesized that there are some enteric bacteria which can specifically capture norovirus particles through HBGA-like substances. As a result, I successfully isolated an enteric bacterial strain, Enterobacter sp. SENG-6, as an HBGA-positive bacteria. The binding between this strain and norovirus-like particles (NoVLPs) were confirmed by transmission electron microscopy (TEM). I also confirmed that Enterobacter sp. SENG-6 is excreting A-like substances as extracellular polymeric substances. These results revealed that HBGA-positive bacteria can affect the fate of human noroviruses by capturing via HBGA-like substances.

Research Products

• [Journal Article] Histo-blood group antigen-like substances of human enteric bacteria as specific adsorbents for human noroviruses (2013)
  • Author(s): Takayuki Miura, Daisuke Sano, Atsushi Suenaga, Takeshi Yoshimura, Miyu Fuzawa, Toyoko Nakagomi, Osamu Nakagomi and Satoshi Okabe
  • Journal Title: Journal of Virology Volume: 87(17) Pages: 9441-9451
• [Journal Article] 生活用水に潜むウイルス対策 (2013)
  • Author(s): 佐野大輔
  • Journal Title: バムサジャーナル Volume: 25(4) Pages: 8-13
• [Journal Article] 水環境中の病原ウイルス分析手法 (2013)
  • Author(s): 三浦尚之, 佐野大輔
  • Journal Title: 水環境学会誌 Volume: 36(5) Pages: 151-155
• [Journal Article] 感染性胃腸炎と上下水道の衛生工学的管理 (2011)
  • Author(s): 佐野大輔
  • Journal Title: 化学療法の領域 Volume: 4 Pages: 82-88
• [Journal Article] 水系管理によるノロウイルス感染症の制御の可能性 (2010)
  • Author(s): 佐野大輔
  • Journal Title: 感染対策ICT ジャーナル Volume: 5(4) Pages: 421-426
• [Presentation] Norovirus-binding enteric bacteria : significance in the environmental dissemination of gastroenteritis viruses (2014)
  • Author(s): Daisuke Sano
  • Organizer: The 2nd Global Leadership Initiative Special Workshop on Water Virology
  • Place of Presentation: Kusatsu, Gunma, Japan
  • Year and Date: 2014-01-21
• [Presentation] Bacterial extracellular polymeric substances as the specific carrier for human norovirus particles (2013)
  • Author(s): Takeshi Yoshimura, Daisuke Sano, Takayuki Miura, Toyoko Nakagomi, Osamu Nakagomi and Satoshi Okabe
  • Organizer: WaterMicro2013. Florianopolis island
  • Place of Presentation: Santa Catariana, Brazil
  • Year and Date: 20130915-20
• [Presentation] Microfiltration of human noroviruses : Effects of the presence of human enteric bacteria on virus rejection (2013)
  • Author(s): Mohan Amarasiri, Daisuke Sano, Takeshi Yoshimura, So Ishizaki, Toyoko Nakagomi, Osamu Nakagomi and Satoshi Okabe
  • Organizer: WET2013. Tokyo University of Agriculture and Technology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 20130615-16
• [Presentation] A hidden relationship between norovirus and enteric bacteria : norovirus exploits histo-blood group antigen-like substances on bacterial cells for its better survival in nature? (2013)
  • Author(s): Daisuke Sano, Takayuki Miura, Takeshi Yoshimura, Miyu Fuzawa, Toyoko Nakagomi, Osamu Nakagomi and Satoshi Okabe
  • Organizer: The 47th Joint Working Conference on Viral Diseases, US-Japan Cooperative Medical Science Program
  • Place of Presentation: Mandarin Oriental Singapore, Singapore
  • Year and Date: 20130312-13
• [Presentation] 囗Enteric bacteria bearing histo-blood group antigen-like extracellular polymeric substances as environmental vehicles for human noroviruses (2012)
  • Author(s): Takayuki Miura, Daisuke Sano, Atsushi Suenaga, Takeshi Yoshimura, Toyoko Nakagomi, Osamu Nakagomi and Satoshi Okabe
  • Organizer: 3rd Food and Environmental Virology Conference
  • Place of Presentation: Lisbon, Portugal
  • Year and Date: 20121007-10
• [Presentation] Microbiologically safe water management in membrane-based wastewater reuse (2012)
  • Author(s): Daisuke Sano and Satoshi Okabe
  • Organizer: The 1st symposium on innovation of water monitoring system with rapid, highly precise and exhaustive pathogen detection technologies
  • Place of Presentation: Sendai, Miyagi, Japan
  • Year and Date: 2012-10-20
• [Presentation] Bacterial extracellular polymeric substances as a novel adsorbent for human norovirus (2012)
  • Author(s): Takeshi Yoshimura, Daisuke Sano, Atsushi Suenaga, Takayuki Miura and Satoshi Okabe
  • Organizer: Japan and China Joint Symposium, Sustainable Sanitation and Environment and Human Health Risk Management
  • Place of Presentation: Sapporo, Hokkaido, Japan
  • Year and Date: 2012-10-15
• [Presentation] Human norovirus-binding enteric bacteria bearing histo-blood group antigen-like extracellular polymeric substances (2012)
  • Author(s): Daisuke Sano
  • Organizer: The 46th Joint Working Conference on Viral Diseases, US-Japan Cooperative Medical Science Program
  • Place of Presentation: Beppu, Oita, Japan
  • Year and Date: 2012-06-19
• [Presentation] Fate of enteric viruses in a membrane bioreactor (2011)
  • Author(s): Takayuki Miura, Atsuhiro Tsutsumi, Daisuke Sano and Satoshi Okabe
  • Organizer: The Young Researchers and Students Symposium of NSFC-JST Joint Research Program
  • Place of Presentation: Xi'an, China
  • Year and Date: 2011-03-04
• [Presentation] Human enteric bacteria that capture norovirus particles with a specific interaction through histo-blood group antigen-like moiety (2010)
  • Author(s): Daisuke Sano, Atsushi Suenaga, Toyoko Nakagomi, Osamu Nakagomi and Satoshi Okabe
  • Organizer: The 2nd COST 929 Symposium
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 20101007-09
• [Presentation] Norovirus-binding bacteria : significance in the environmental dissemination of gastroenteritis viruses (2010)
  • Author(s): Daisuke Sano
  • Organizer: The 44th Joint Working Conference on Viral Diseases, US-Japan Cooperative Medical Science Program
  • Place of Presentation: Hokkaido University, Sapporo, Japan
  • Year and Date: 20100628-30
• [Remarks] ホームページ
  • URL: http://www.eng.hokudai.ac.jp/labo/water/index.html
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• [Remarks] 北海道新聞(7月2日朝刊)「ノロ吸着する腸内細菌 北大グループ特定 治療薬開発に道」
• [Remarks] 北海道医療新聞(7月19日)「ノロウイルス吸着腸内細菌を同定-感染予防開発へ期待 北大(工)佐野准教授ら」
• [Remarks] TV ニュースNHK・おはよう北海道・土曜プラス(6月29日)
• [Remarks] NET ニュースNHK NEWS WEB(6月29日)ノロウイルスの性質解明 薬の開発に
  • URL: http://archive.today/QdyIk