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2011 Fiscal Year Final Research Report

Cell selection based on genetic signal

Research Project

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Project/Area Number 22686077
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Biofunction/Bioprocess
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

ABE Hiroshi  独立行政法人理化学研究所, 伊藤ナノ医工学研究室, 専任研究員 (80415067)

Project Period (FY) 2010 – 2011
KeywordsRNA / 細胞 / 分離 / イメージング / 蛍光 / 化学反応 / フローサイトメーター / DNA
Research Abstract

Our aim is to develop new RNA detection probe which is capable of cell selection based on genetic signal. First, we synthesized red fluorescent probe to offer very high signal and background ratio. Next, the probe was designed to target miRNA which is expressed in stem cells. As a result, we confirmed specific miRNA signal in model cells. Now, we are trying to isolate cells with specific miRNA signal.

  • Research Products

    (12 results)

All 2012 2011 2010 Other

All Journal Article (5 results) Presentation (4 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Universal Caging Group for In Cell Detection of Glutathione Transferase Applied to 19F NMR and Bioluminogenic Probes2012

    • Author(s)
      Mika Ito, Aya Shibata, Jie Zhang, Michio Hiroshima, Yasushi Sako, Yukiko Nakano, Kyoko Kojima-Aikawa, Bengt Mannervik, Satoshi Shuto, Yoshihiro Ito, Ralf Morgenstern, Hiroshi Abe
    • Journal Title

      ChemBioChem

      Volume: (in press)

  • [Journal Article] Oligonucleotide-Templated, Reactions for Sensing Nucleic Acids2012

    • Author(s)
      Aya Shibata, Hiroshi Abe, Yoshihiro Ito
    • Journal Title

      Molecules

      Volume: 17(3) Pages: 2446-2463

  • [Journal Article] Fluorescent Detection of Intron Lariat RNAs with Reduction-Triggered Fluorescence Probes2011

    • Author(s)
      Kazuhiro Furukawa, Hiroshi Abe, Yasutsugu Tamura, Rei Yoshimoto, Minoru Yoshida, Satoshi Tsuneda, Yoshihiro Ito
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 50(50) Pages: 12020-12023

  • [Journal Article] Synthesis and characterization of a series of highly fluorogenic substrates for glutathione transferases, a general strategy2011

    • Author(s)
      Jie Zhang, Aya Shibata, Mika Ito, Satoshi Shuto, Yoshihiro Ito, Bengt Mannervik, Hiroshi Abe and Ralf Morgenstern
    • Journal Title

      Journal of the American Chemical Society

      Volume: 133(35) Pages: 14109-14119

  • [Journal Article] Ralf Morgenstern, Characterization of New Potential Anticancer Drugs Designed To Overcome Glutathione Transferase Mediated Resistance2011

    • Author(s)
      Katarina Johansson, Mika Ito, Carolien M. S. Schophuizen, Sherin Mathew T, Vanina D. Heuser, Jie Zhang, Miyuki Shimoji, Marie Vahter, Wee Han Ang, Paul J. Dyson, Aya Shibata, Satoshi Shuto, Yoshihiro, Ito, Hiroshi Abe
    • Journal Title

      Molecular Pharmaceutics.

      Volume: 8(5) Pages: 1698-1708

  • [Presentation] 生細胞内遺伝子の解析と制御を目的とした機能正核酸分子の創製2012

    • Author(s)
      阿部洋
    • Organizer
      次代を担う若手大学人育成イニシアティブセミナー
    • Place of Presentation
      筑波大学下田臨界実験センター、下田市
    • Year and Date
      2012-01-13
  • [Presentation] 遺伝子シグナルの化学的増幅2011

    • Author(s)
      阿部洋
    • Organizer
      トクシマ・ファルマ・トライアングル(TPT)構築事業特別講演会
    • Place of Presentation
      徳島大学薬学部、徳島市
    • Year and Date
      2011-12-07
  • [Presentation] 医療応用を目指した人工核酸分子の創製2011

    • Author(s)
      阿部洋
    • Organizer
      高分子学会関東支部第22回埼玉地区談話会
    • Place of Presentation
      東洋大学川越キャンパス、川越市
    • Year and Date
      2011-02-17
  • [Presentation] 人工核酸分子を用いた遺伝子発現の解析と制御2010

    • Author(s)
      阿部洋
    • Organizer
      大阪大学免疫学フロンティア研究センターセミナー
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-07-15
  • [Book] 「生細胞内RNA検出のための化学反応プローブ」「難培養微生物研究の最新技術II~ゲノム解析を中心とした最前線と将来展望~」2010

    • Author(s)
      古川和寛、阿部洋、伊藤嘉浩、常田聡
    • Total Pages
      19-27
    • Publisher
      シーエムシー出版
  • [Remarks]

    • URL

      http://www.riken.jp/nano-med.eng.lab/abe.html

  • [Patent(Industrial Property Rights)] 蛍光発生分子2011

    • Inventor(s)
      阿部洋、伊藤嘉浩、古川和寛
    • Industrial Property Rights Holder
      理化学研究所
    • Industrial Property Number
      US8084201
    • Acquisition Date
      2011-03-31
    • Overseas

URL: 

Published: 2013-07-31  

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