Analysis of DNA repair pathway by the combination of proteomics andgenetic approach

2012 Fiscal Year Final Research Report

• Project/Area Number: 22687001
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: Tokyo Metropolitan University (2012); Kyoto University (2010-2011)
• Principal Investigator: HIROTA Kouji 首都大学東京, 大学院・理工学研究科, 教授 (00342840)
• Project Period (FY): 2010 – 2012
• Keywords: DNA修復 / DNA損傷応答 / クロマチン / ユビキチン

Research Abstract

In this study, we analyzed ubiquitylation signal pathway in DNA repair using the combination of proteomics and genetic approach. Our data figure out novel ubiquitin signal pathway, which is useful seeds targets for cancer chemotherapy.

Research Products

• [Journal Article] nterference in DNA replication can cause mitotic chromosomal breakage unassociated with double-strand breaks (2013)
  • Author(s): FujitaM, Sasanuma H, Yamamoto K.N, Harada H, Kurosawa A, Adachi N, Omura M, Hiraoka M, Takeda S, Hirota K
  • Journal Title: PLOS ONE
• [Journal Article] Structure-specific endonucleases Xpf and Mus81 play overlapping but essential roles in DNA repair by homologous recombination (2013)
  • Author(s): Kikuchi, K, Narita, T., Van, P.T., Iijima, J., Hirota, K., Keka, I.S.,Mohiuddin, Okawa, K., Hori, T., Fukagawa, T., Essers, J., Kanaar, R.,Whitby, M.C., Sugasawa, K., Taniguchi, Y., Kitagawa, K., and Takeda, S
  • Journal Title: Cancer Research
• [Journal Article] A Central Coupler for Recombination Initiation Linking Chromosome Architecture to S Phase Checkpoint (2012)
  • Author(s): Miyoshi T, Ito M, Kugou K, Yamada S, Furuichi M, Oda A, Yamada T, Hirota K,Masai H, Ohta K
  • Journal Title: Mol. Cell Volume: 47(5) Pages: 722-733
• [Journal Article] The USP1/UAF1 Complex Promotes Double-Strand Break Repair through Homologous Recombination. (2011)
  • Author(s): J. Murai, K. Yang, D. Dejsuphong, K. Hirota, S. Takeda and A. D. D'Andrea
  • Journal Title: Molecular and cellular biology Volume: 31 Pages: 2462-2469
• [Journal Article] The Epistatic Relationship between BRCA2 and the Other RAD51 Mediators in Homologous Recombination. (2011)
  • Author(s): Y. Qing, M. Yamazoe, K. Hirota, D. Dejsuphong, W.Sakai, K. N. Yamamoto, D. K. Bishop, X. Wu and S. Takeda
  • Journal Title: PLoS genetics Volume: 7 Pages: e1002148
• [Journal Article] Characterization of environmental chemicals with potential for DNA damage using isogenic DNA repair-deficient chicken DT40 cell lines (2011)
  • Author(s): K. N. Yamamoto, K. Hirota, K. Kono, S. Takeda, S. Sakamuru,M. Xia, R. Huang, C. P. Austin, K. L. Witt and R. R. Tice
  • Journal Title: Environmental and molecular mutagenesis
• [Journal Article] Involvement of SLX4 in interstrand cross-link repair is regulated by the Fanconi anemia pathway. (2011)
  • Author(s): K. N. Yamamoto, S. Kobayashi, M. Tsuda, H. Kurumizaka, M. Takata, K. Kono, J. Jiricny, S. Takeda and K. Hirota
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108 Pages: 6492-6496
• [Journal Article] Purification of the human SMN-GEMIN2 complex and assessment of its stimulation of RAD51-mediated DNA recombination reactions (2011)
  • Author(s): Tskaku M., Tsujita T., Horikoshi N., Takizawa Y., Qing Y., Hirota K., Ikura T., Takeda S., *Kurumizaka H
  • Journal Title: Biochemistry Volume: 32 Pages: 6797-6805
• [Journal Article] Mutant cells defective in DNA repair pathways provide a sensitive high-throughput assay for genotoxicity (2010)
  • Author(s): T. J. Evans, K. N. Yamamoto, K. Hirota and S.Takeda
  • Journal Title: DNA repair 9 Pages: 1292-1298
• [Journal Article] Simultaneous disruption of two DNA polymerases, Poleta and Polzeta, in Avian DT40 cells unmasks the role of Poleta in cellular response to various DNA lesions (2010)
  • Author(s): K. Hirota, E. Sonoda, T. Kawamoto, A. Motegi, C. Masutani, F. Hanaoka, D. Szuts, S. Iwai, J. E. Sale, A.Lehmann and S. Takeda
  • Journal Title: PLoS genetics Volume: 6
• [Journal Article] DNA polymerases nu and theta are required for efficient immunoglobulin V gene diversification in chicken (2010)
  • Author(s): M. Kohzaki, K. Nishihara, K. Hirota, E. Sonoda, M. Yoshimura, S. Ekino, J. E. Butler, M. Watanabe, T. D. Halazonetis and S. Takeda
  • Journal Title: The Journal of cell biology Volume: 189 Pages: 1117-1127
• [Journal Article] Human replicative DNA polymerase delta can bypass T-T (6-4) ultraviolet photoproducts on template strands (2010)
  • Author(s): T. Narita, T. Tsurimoto, J. Yamamoto, K. Nishihara, K. Ogawa, E. Ohashi, T. Evans, S. Iwai, S. Takeda and K. Hirota
  • Journal Title: Genes Cells Volume: 15 Pages: 1228-1239
• [Journal Article] The roles of stress-activated Sty1 and Gcn2 kinases and of the protooncoprotein homologue Int6/eIF3e in responses to endogenous oxidative stress during histidine starvation (2010)
  • Author(s): N. Nemoto, T. Udagawa, T. Ohira, L. Jiang, K. Hirota, C. R. Wilkinson, J. Bahler, N. Jones, K. Ohta, R. C. Wek and K. Asano
  • Journal Title: Journal of molecular biology Volume: 404 Pages: 183-201
• [Journal Article] GEMIN2 promotes accumulation of RAD51 at double-strand breaks in homologous recombination (2010)
  • Author(s): Y. Takizawa, Y. Qing, M. Takaku, T. Ishida, Y. Morozumi, T. Tsujita, T. Kogame, K. Hirota, M. Takahashi, T. Shibata, H. Kurumizaka and S. Takeda
  • Journal Title: Nucleic acids research
• [Journal Article] KIAA1018/FAN1 nuclease protects cells against genomic instability induced by interstrand cross-linking agents (2010)
  • Author(s): K. Yoshikiyo, K. Kratz, K. Hirota, K. Nishihara, M. Takata, H. Kurumizaka, S. Horimoto, S. Takeda and J. Jiricny
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 Pages: 21553-21557
• [Presentation] SUMO-targetted Ubiquitin Ligase RNF4 Guards Genome Stability by suppressing error-prone Homologous Recombination (2012)
  • Author(s): Kouji Hirota at al
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 博多
  • Year and Date: 2012-12-14
• [Presentation] The involvement of SLX4 in interstrand cross-link repair is regulated by the Fanconi anemia pathway. (2011)
  • Author(s): Kouji Hirota at al.
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] DNAポリメラーゼδによるAP部位の損傷乗越え (2010)
  • Author(s): 廣田耕志
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-22
• [Book] 血液内科領域における抗腫瘍薬の作用機序・副作用に基づく使い分け (2012)
  • Author(s): 笹沼博之、廣田耕志、武田俊
  • Total Pages: 65(4)498-503
  • Publisher: シスプラチン耐性の分子機構 月刊血液内科
• [Book] 廣田耕志、武田俊一 (2011)
  • Author(s): シスプラチン耐性の分子機構
  • Total Pages: 239(4) 302-304
  • Publisher: 医学の歩み
• [Book] 基礎の基礎 (2010)
  • Author(s): 廣田耕志、武田俊一
  • Total Pages: 29-(1)14-20
  • Publisher: 細胞工学
• [Remarks] プレスリリース発表2013 4/4DNAが切れていないのに発生する染色体断裂の発見-ヒトの被爆線量を測定する手法に異議あり-
  • URL: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2013/130404_1.htm