Improvement of the membrane permeability of compounds used by streptothricin biosynthetic enzymes

2012 Fiscal Year Final Research Report

• Project/Area Number: 22688007
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Applied microbiology
• Research Institution: Fukui Prefectural University
• Principal Investigator: HAMANO Yoshimitsu 福井県立大学, 生物資源学部, 准教授 (50372834)
• Project Period (FY): 2010 – 2012
• Keywords: NRPS / ペプチド合成酵素

Research Abstract

The streptothricin (ST) antibiotics, produced by Streptomyces bacteria, contain L-β-lysine oligopeptides as pendant chains. In this study, we identified three unusual nonribosomal peptide synthetases (NRPSs) involved in ST biosynthesis: ORF 5 (a stand-alone adenylation (A) domain), ORF 18 (containing thiolation (T) and condensation (C) domains) and ORF 19 (a stand-alone A domain).

Research Products

• [Journal Article] 抗生物質ストレプトスリシンの修飾酵素と生合成酵素による毒性緩和 (2013)
  • Author(s): 濱野吉十
  • Journal Title: バイオサイエンスとインダストリー(財団法人バイオインダストリー協会) Volume: Vol. 71,No. 1 Pages: 32-35
  • URL: http://www.jba.or.jp/pc/archive/2013/vol71_no1.html
  • Peer Reviewed
• [Journal Article] A stand-alone adenylation domain forms amide bonds in streptothricin biosynthesis (2012)
  • Author(s): Chitose Maruyama, Junya Toyoda, YasYasuo Kato, Miho Izumikawa, Motoki TakYasuo Kato, Miho Izumikawa, Motoki Takagi, Kazuo Shin-ya, Hajime Katano, Takashi Utagawa, and Yoshimitsu Hamano
  • Journal Title: Nature Chem. Biol Volume: 8 Pages: 791-797
  • DOI: DOI:10.1038/nchembio.1040
  • Peer Reviewed
• [Journal Article] NRPSs and amide ligases producing homopoly(amino acid)s and homooligo(amino acid)s
  • Author(s): 濱野吉十
  • Journal Title: Natural Product Reports Volume: (In press)
  • DOI: DOI:10.1039/C3NP70025A
  • Peer Reviewed
• [Presentation] Streptothricin (ST)生合成酵素群を利用した新規化合物の創製 (2012)
  • Author(s): 濱野吉十
  • Organizer: 日本生物工学会
  • Place of Presentation: 神戸
  • Year and Date: 2012-10-23
• [Presentation] A stand-alone adenylation domain catalyzes multiple amide-bond formation in streptothricin biosynthesis (2012)
  • Author(s): 濱野吉十
  • Organizer: Directing Biosynthesis III
  • Place of Presentation: Nottingham (UK)
  • Year and Date: 2012-09-19
• [Presentation] Streptothricin(ST)生合成酵素群を利用した新規ST類縁化合物の創製 (2012)
  • Author(s): 濱野吉十
  • Organizer: 日本放線菌学会
  • Place of Presentation: 東京
  • Year and Date: 2012-09-06
• [Presentation] A stand-alone adenylation domain catalyzes multiple amide-bond formation in streptothricin biosynthesis (2012)
  • Author(s): 濱野吉十
  • Organizer: Society of Industrial Microbiology and Biotechnology (SIMB) 2012 meeting(招待講演)
  • Place of Presentation: Washington DC (US)
  • Year and Date: 2012-08-12
• [Presentation] ホモポリアミノ酸構造を有する天然生理活性物質の生合成機構と有用物質生産への展開 (2012)
  • Author(s): 濱野吉十
  • Organizer: 第2回生合成マシナリー札幌セミナー(招待講演)
  • Place of Presentation: 札幌
  • Year and Date: 2012-05-24
• [Presentation] Streptothricin(ST)生合成における非リボソームペプチド合成酵素の機能解析 (2012)
  • Author(s): 丸山千登勢
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-24
• [Presentation] ホモポリアミノ酸構造を有する天然生理活性物質の生合成機構と有用物質生産への展開 (2012)
  • Author(s): 濱野吉十
  • Organizer: 東京大学薬学部講演会(招待講演)
  • Place of Presentation: 東京
  • Year and Date: 2012-02-09
• [Presentation] Unusual Nonribosomal Peptide Synthetase Involved in the Biosynthesis of a Classic Antibiotic, Streptothricine (2011)
  • Author(s): 丸山千登勢
  • Organizer: International Symposium on the Biology of Actinomycetes
  • Place of Presentation: Puerto Vallarta (Mexico)
  • Year and Date: 2011-12-12
• [Presentation] Streptothricin (ST)生合成酵素群を利用した新規化合物の創製 (2011)
  • Author(s): 丸山千登勢
  • Organizer: 日本生物工学会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-27
• [Presentation] Streptothricin (ST)生合成酵素群を利用した新規化合物の創製 (2011)
  • Author(s): 丸山千登勢
  • Organizer: 日本放線菌学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-08
• [Presentation] Streptothricin(ST)生合成遺伝子群の機能解析 (2011)
  • Author(s): 丸山千登勢
  • Organizer: 日本農芸化学会
  • Place of Presentation: 学会中止のため要旨集にて発表
  • Year and Date: 2011-03-05
• [Presentation] β-リジンホモオリゴマー(β-LO)生産放線菌の構築 (2011)
  • Author(s): 豊田順也
  • Organizer: 日本農芸化学会
  • Place of Presentation: 学会中止のため要旨集にて発表
  • Year and Date: 2011-03-05
• [Presentation] Identification of the peptide synthetase involved in the Streptothricin biosynthesis (2010)
  • Author(s): 丸山千登勢
  • Organizer: Pacifichem 2010
  • Place of Presentation: ハワイ(アメリカ)
  • Year and Date: 2010-12-17
• [Presentation] Unusual non-ribosomal peptide synthetases producing amino-acid homopolymers (2010)
  • Author(s): 濱野吉十
  • Place of Presentation: ハワイ(アメリカ)
  • Year and Date: 2010-12-16
• [Presentation] ホモポリアミノ酸構造を有する天然生理活性物質の生合成機構と有用物質生産への展開 (2010)
  • Author(s): 濱野吉十
  • Organizer: 東京大学生物生産工学研究センターシンポジウム
  • Place of Presentation: 東京大学
  • Year and Date: 2010-12-08
• [Presentation] Streptothricin(ST)生合成を担う新規非リボソームペプチド合成酵素 (2010)
  • Author(s): 丸山千登勢
  • Organizer: 日本生物工学会
  • Place of Presentation: 宮崎市
  • Year and Date: 2010-10-28
• [Remarks]
  • URL: http://www.s.fpu.ac.jp/hamano/