2013 Fiscal Year Final Research Report
Development of technique to disrupt the reproductive function of Monochamus alternatus using an endosymbiotic bacterium, Wolbachia
| Project/Area Number |
22688012
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Forest science
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
AIKAWA Takuya 独立行政法人森林総合研究所, 東北支所, 主任研究員 (90343805)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | マツノマダラカミキリ / ボルバキア / マイクロインジェクション / 生殖異常 / マツ材線虫病 |
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| Research Abstract |
Wolbachia are endosymbiotic bacteria in arthropods including insects, and capable of manipulating host reproduction by causing cytoplasmic incompartibility, parthenogenesis, male killing or feminization. In this study, effect of artificial injection of the Wolbachia of Collosobruchus chinensis (cause cytoplasmic incompartibility to the host) to Monochamus alternatus was investigated. Mating experiments between Wolbachia-injected individuals and non-injected individuals revealed that only the hatching rate of eggs produced by mating of injected males x non-injected females decreased remarkably. This means that the Wolbachia of C. chinensis caused cytoplasmic incompartibility to M. alternatus.
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