2013 Fiscal Year Final Research Report
PIPs and Ca2+-dependent regulation on Ca channels
| Project/Area Number |
22689006
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Kyoto University (2012-2013)
Fukuoka University (2010-2011) |
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Principal Investigator |
MASAYUKI Mori 京都大学, 工学(系)研究科(研究院), 准教授 (80342640)
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| Co-Investigator(Renkei-kenkyūsha) |
INOUE Ryuji 福岡大学, 医学部, 教授 (30232573)
OKAMURA Yasushi 大阪大学, 医学部, 教授 (80201987)
MORI Yasuo 京都大学, 地球環境学童, 教授 (80212265)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 生理学 / ホスホイノシチド / カルシウム / TRP channel / PIP2 / 平滑筋 / 蛍光共鳴エネルギー移動 / シミュレーション |
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| Research Abstract |
TRPC3/C6/C7 channels, a subgroup of classical/canonical TRP channels, are activated by diacylglycerol produced via activation of phospholipase C (PLC)-coupled receptors. The critical importance of PIPs to various ion-transporting molecules is well documented, but their function in relation to these channels remains controversial. We used voltage-sensing phosphatase (DrVSP) to study TRPC3/C6/C7 regulation and found that the channel activity was controlled by PIP2-DAG signaling in a self-limiting manner. We also simulated the channel dynamics based on this self-limiting hypothesis. Overall, our studies demonstrate a fundamental regulatory mechanism of TRPC3/6/7 activation by PIP2-DAG signal.
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[Journal Article] A human Dravet syndrome model from patient induced pluripotent stem cells2013
Author(s)
Higurashi N, Uchida T, Lossin C, Misumi Y, Okada Y, Akamatsu W, Imaizumi Y, Zhang B, Nabeshima K, Mori MX, Katsurabayashi S, Shirasaka Y, Okano H, Hirose S
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Journal Title
Mol Brain
Volume: 2, 6
Pages: 19
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