The development of cardiomyocyte induction method

2012 Fiscal Year Final Research Report

• Project/Area Number: 22689027
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Circulatory organs internal medicine
• Research Institution: Keio University
• Principal Investigator: YUASA Shinsuke 慶應義塾大学, 医学部, 講師 (90398628)
• Project Period (FY): 2010 – 2012
• Keywords: 分子心臓病態学

Research Abstract

The direct induction of cardiomyocyte from fibroblast is highly desired for cardiac regenerative medicine. The combination of cardiac transcription factors successfully induces cardiomyocyte redirection from fibroblast. Quantitative RT-PCR analysis showed

Research Products

• [Journal Article] The generation of induced pluripotent stem cells from a patient with KCNH2 G603D, without LQT2 disease associated symptom (2013)
  • Author(s): Okata S, Yuasa S, Yamane T, Furukawa T, Fukuda K.
  • Journal Title: J Med Dent Sci Volume: 60 Pages: 17-22
  • Peer Reviewed
• [Journal Article] Anatomical variations affect radial artery spasm and procedural achievement of transradial cardiac catheterization (2013)
  • Author(s): Numasawa Y, Kawamura A, Kohsaka S, Takahashi M, Endo A, Arai T, Ohno Y, Yuasa S, Maekawa Y, Fukuda K
  • Journal Title: Heart Vessels
  • URL: http://link.springer.com/article/10.1007/s00380-013-0324-3/fulltext.html
  • Peer Reviewed
• [Journal Article] Novel insights into disease modeling using induced pluripotent stem cells. (2013)
  • Author(s): Egashira T, Yuasa S, Fukuda K.
  • Journal Title: Biol Pharm Bull Volume: 36(2) Pages: 182-8
  • Peer Reviewed
• [Journal Article] Distinct Metabolic Flow Enables Large-Scale Purification of Mouse and Human Pluripotent Stem Cell-Derived Cardiomyocytes (2013)
  • Author(s): Tohyama S, Hattori F, Sano M, Hishiki T, Nagahata Y, Matsuura T, Hashimoto H, Suzuki T, Yamashita H, Satoh Y, Egashira T, Seki T, Muraoka N, Yamakawa H, Ohgino Y, Tanaka T, Yoichi M, Yuasa S, Murata M, Suematsu M, Fukuda K.
  • Journal Title: Cell Stem Cell Volume: 12 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Acute coronary syndrome or apical ballooning syndrome (2013)
  • Author(s): Maekawa Y, Kawamura A, Yuasa S, Ohno Y, Arai T, Fukuda K
  • Journal Title: Heart Vessels Volume: 28(1) Pages: 130-3
  • Peer Reviewed
• [Journal Article] Incidence of periprocedural myocardial infarction and cardiac biomarker testing after percutaneous coronary intervention in Japan: results from a multicenter registry. (2012)
  • Author(s): Arai T, Yuasa S, Miyata H, Kawamura A, Maekawa Y, Ishikawa S, Noma S, Inoue S, Sato Y, Kohsaka S, Fukuda K
  • Journal Title: Heart Vessels
  • URL: http://link.springer.com/article/10.1007%2Fs00380-012-0314-x
  • Peer Reviewed
• [Journal Article] miR-142-3p is essential for hematopoiesis and affects cardiac cell fate in zebrafish (2012)
  • Author(s): Nishiyama T, Kaneda R, Ono T, Tohyama S, Hashimoto H, Endo J, Tsuruta H, Yuasa S, Ieda M, Makino S, Fukuda K
  • Journal Title: Biochem Biophys Res Commun. Volume: 425(4) Pages: 755-61
  • Peer Reviewed
• [Journal Article] Disease characterization using LQTS-specific induced pluripotent stem cells. (2012)
  • Author(s): Egashira T, Yuasa S, Suzuki T, Aizawa Y, Yamakawa H, Matsuhashi T, Ohno Y, Tohyama S, Okata S, Seki T, Kuroda Y, Yae K, Hashimoto H, Tanaka T, Hattori F, Sato T, Miyoshi S, Takatsuki S, Murata M,Kurokawa J, Furukawa T, Makita N, Aiba T, Shimizu W, Horie M
  • Journal Title: Cardiovasc Res Volume: 95(4) Pages: 419-29
  • Peer Reviewed
• [Journal Article] Generation of induced pluripotent stem cells from a small amount of human peripheral blood using a combination of activated T cells and Sendai virus. (2012)
  • Author(s): Seki T, Yuasa S, Fukuda K
  • Journal Title: Nature Protocols Volume: 7(4) Pages: 718-28
  • Peer Reviewed
• [Journal Article] Direct comparison of Takotsubo cardiomyopathy between Japan and USA: 3-year follow-up study (2012)
  • Author(s): Maekawa Y, Kawamura A, Yuasa S, Nesto RW, Fukuda K
  • Journal Title: Intern Med. Volume: 51(3) Pages: 257-62
  • Peer Reviewed
• [Journal Article] Wnt2 accelerates cardiac myocyte differentiation from ES-cell derived mesodermal cells via non-canonical pathway. (2012)
  • Author(s): Onizuka T, Yuasa S, Kusumoto D, Shimoji K, Egashira T, Ohno Y, Kageyama T, Tanaka T, Hattori F, Fujita J, Ieda M, Kimura K, Makino S, Sano M, Kudo A, Fukuda K.
  • Journal Title: J Mol Cell Cardiol Volume: 52(3) Pages: 650-9
  • Peer Reviewed
• [Journal Article] A case of severe aortic stenosis with severe coronary artery disease that was successfully treated by balloon aortic valvuloplasty and percutaneous coronary intervention (2012)
  • Author(s): Maekawa Y, Kawamura A, Furuta A, Yua sa S, Fukuda K
  • Journal Title: Heart Vessels Volume: 27(5) Pages: 528-31
  • Peer Reviewed
• [Journal Article] Successful percutaneous coil embolization of coronary-pulmonary, -carotid, and -internal mammary artery fistulas (2012)
  • Author(s): Numasawa Y, Kawamura A, Hashimoto S, Endo A, Yuasa S, Maekawa Y, Kuribayashi S, Fukuda K
  • Journal Title: Heart Vessels. Volume: 27(3) Pages: 331-6
  • Peer Reviewed
• [Journal Article] Successful coronary intervention of chronic total occlusion of the right coronary artery by ipsilateral injection via an isolated conus artery. (2012)
  • Author(s): Arai T, Kawamura A, Yuasa S, Maekawa Y, Fukuda K.
  • Journal Title: Heart Vessels. Volume: 27(3) Pages: 327-30
  • Peer Reviewed
• [Journal Article] Effect of preoperative evaluation by multidetector computed tomography in percutaneous coronary interventions of chronic total occlusions. (2012)
  • Author(s): Ueno K, Kawamura A, Onizuka T, Kawakami T, Nagatomo Y, Hayashida K, Yuasa S, Maekawa Y, Anzai T, Jinzaki M, Kuribayashi S, Ogawa S
  • Journal Title: Int J Cardiol Volume: 156(1) Pages: 76-9
  • Peer Reviewed
• [Journal Article] Induced pluripotent stem cells in cardiovascular medicine (2011)
  • Author(s): Egashira T, Yuasa S, Fukuda K
  • Journal Title: Stem Cells Int Volume: 2011 Pages: 348960
  • Peer Reviewed
• [Journal Article] Derivation of induced pluripotent stem cells from human peripheral circulating T cells (2011)
  • Author(s): Seki T, Yuasa S, Fukuda K
  • Journal Title: Curr Protoc Stem Cell Biol
  • Peer Reviewed
• [Journal Article] Outcomes of Intravascular Ultrasound-Guided Percutaneous Coronary Intervention With Drug-Eluting Stents Versus Bare Metal Stents for Acute Coronary Syndrome in Octogenarians (2011)
  • Author(s): Maekawa Y, Kawamura A, Yuasa S, Ohno Y, Arai T, Numasawa Y, Endo A, Fukuda K
  • Journal Title: Angiology Volume: 62(8) Pages: 620-4
  • Peer Reviewed
• [Journal Article] G-CSF influences mouse skeletal muscle development and regeneration by stimulating myoblast proliferation. (2011)
  • Author(s): Hara M, Yuasa S, Shimoji K, Onizuka T, Hayashiji N, Ohno Y, Arai T, Hattori F, Kaneda R, Kimura K, Makino S, Sano M,Fukuda K.
  • Journal Title: J Exp Med. Volume: 208(4) Pages: 715-27
  • Peer Reviewed
• [Journal Article] Neural crest-derived stem cells migrate and differentiate into cardiomyocytes after myocardial infarction. (2011)
  • Author(s): Tamura Y, Matsumura K, Sano M, Tabata H, Kimura K, Ieda M, Arai T, Ohno Y, Kanazawa H, Yuasa S, Kaneda R, Makino S, Nakajima K, Okano H, Fukuda K
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 31(3) Pages: 582-9
  • Peer Reviewed
• [Journal Article] Simple autogeneic feeder cell preparation for pluripotent stem cells. (2011)
  • Author(s): Li W, Yamashita H, Hattori F, Chen H, Tohyama S, Satoh Y, Sasaki E, Yuasa S, Makino S, Sano M, Fukuda K
  • Journal Title: Stem Cell Res Volume: 6(1) Pages: 83-9
  • Peer Reviewed
• [Journal Article] 4-Hydroxy-2-nonenal protects against cardiac ischemia-reperfusion injury via the Nrf2-dependent pathway. (2010)
  • Author(s): Zhang Y, Sano M, Shinmura K, Tamaki K, Katsumata Y, Matsuhashi T, Morizane S, Ito H, Hishiki T, Endo J, Zhou H, Yuasa S, Kaneda R, Suematsu M, Fukuda K
  • Journal Title: J Mol Cell Cardiol. Volume: 49(4) Pages: 576-586
  • Peer Reviewed
• [Journal Article] Generation of induced pluripotent stem cells from human terminally differentiated circulating T cells. (2010)
  • Author(s): Seki T, Yuasa S, Oda M, Egashira T, Yae K, Kusumoto D, Nakata H, Tohyama S, Hashimoto H, Kodaira M, Okada Y, Seimiya H, Fusaki N, Hasegawa M, Fukuda K
  • Journal Title: Cell Stem Cell. Volume: 7(1) Pages: 11-4
  • Peer Reviewed
• [Journal Article] Zac1 is an essential transcription factor for cardiac morphogenesis. (2010)
  • Author(s): Yuasa S, Onizuka T, Shimoji K, Ohno Y, Kageyama T, Yoon SH, Egashira T, Seki T,Hashimoto H, Nishiyama T, Kaneda R, Murata M, Hattori F, Makino S, Sano M, Ogawa S, Harvey RP, Fukuda K
  • Journal Title: Circ Res Volume: 106(6) Pages: 1083-91
  • Peer Reviewed
• [Journal Article] G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ES and iPS cells. (2010)
  • Author(s): Shimoji K, Yuasa S, Onizuka T, Hattori F, Tanaka T, Hara M, Ohno Y, Chen H, Koshimizu U, Ogawa S, Fukuda K
  • Journal Title: Cell Stem Cell. Volume: 6(3) Pages: 227-37
  • Peer Reviewed
• [Presentation] iPS 細胞を用いた心臓病疾患モデルの構築と病態解.明Symposium. (2013)
  • Author(s): Yuasa S
  • Organizer: The 12^<th>Congress of the Japanese Society for Regenerative Medicine.
  • Place of Presentation: Yokohama
  • Year and Date: 2013-03-22
• [Presentation] The Cardiovascular Disease Modeling by using iPS cells (2013)
  • Author(s): Yuasa S
  • Organizer: The Japanese Circulation Society
  • Place of Presentation: Yokohama.
  • Year and Date: 2013-03-16
• [Presentation] The cardiovascular Disease modeling by using iPS cells (2012)
  • Author(s): Yuasa S
  • Organizer: The 29^<th>Annual Meeting of the International Society for Heart Research Japanese section.
  • Place of Presentation: Fukuoka. Symposium.
  • Year and Date: 2012-10-27
• [Presentation] Disease modeling using iPS cells. (2012)
  • Author(s): Yuasa S
  • Organizer: The 60^<th>annual scientific session of the Japanese college of cardiology. Kanazawa
  • Place of Presentation: Panel discussion
  • Year and Date: 2012-09-16
• [Presentation] The establishment of analysis methods and novel therapies for cardiovascular diseases syndrome using human iPS cells (2012)
  • Author(s): Yuasa S
  • Organizer: The Japanese Circulation Society.
  • Place of Presentation: Symposium. Fukuoka.
  • Year and Date: 2012-03-17
• [Presentation] The establishment of analysis methods for cardiovascular genetic diseases using human iPS cells. Symposium (2012)
  • Author(s): Yuasa S
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-15
• [Presentation] Drug evaluation for personalized medicine by using human iPS cells (2011)
  • Author(s): Yuasa S
  • Organizer: The 28th Annual Meeting of the International Society for Heart Research Japanese Section. Symposium
  • Place of Presentation: Tokyo
  • Year and Date: 2011-12-02
• [Presentation] Generation of induced pluripotent stem cells from human terminally differentiated circulating T cells (2011)
  • Author(s): Yuasa S
  • Organizer: Kuopio Stem cell Workshop
  • Place of Presentation: Kuopio, Finland
  • Year and Date: 2011-09-28
• [Presentation] 末梢血最終分化T細胞からのiPS細胞樹立方法の開発 (2011)
  • Author(s): 湯浅慎介
  • Organizer: 日本臨床免疫学会総会 モーニング教育講演
  • Place of Presentation: 東京
  • Year and Date: 2011-09-07
• [Presentation] Generation of Induced Pluripotent Stem Cells from Human Peripheral Blood:Less Invasive and Clinically ApplicableTechnique. (2011)
  • Author(s): Yuasa S
  • Organizer: The Japanese Circulation Society. Symposium.
  • Place of Presentation: Yokohama
  • Year and Date: 2011-08-04
• [Presentation] iPS細胞時代に向けた心筋再生の現状と展望 (2011)
  • Author(s): 湯浅 慎介
  • Organizer: 日本動物細胞工学会2011 年度大会
  • Place of Presentation: Symposium. 東京
  • Year and Date: 2011-07-22
• [Presentation] Generation of induced pluripotent stem cells from human peripheral blood: less invasive and clinically applicable technique (2011)
  • Author(s): Yuasa S
  • Organizer: The 41th Taiwan Society of Cardiology (TSOC) Annual Convention and Scientific Session.
  • Place of Presentation: Taipei, Taiwan.
  • Year and Date: 2011-05-14
• [Presentation] G-CSF regulates skeletal muscle development and regeneration through myoblast-specific expression of the G-CSF receptor (2011)
  • Author(s): 湯浅慎介
  • Organizer: Young Investigator Award. 再生医療学会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-02
• [Presentation] 循環器疾患再生療法開発に向けたiPS 細胞研究 (2010)
  • Author(s): 湯浅慎介
  • Organizer: 心筋生検研究会
  • Place of Presentation: Symposium. 東京
  • Year and Date: 2010-11-27
• [Presentation] 循環器疾患再生療法開発に向けたiPS 細胞研究 (2010)
  • Author(s): 湯浅慎介、福田恵一
  • Organizer: 人工臓器学会大会
  • Place of Presentation: Symposium. 仙台.
  • Year and Date: 2010-11-19
• [Presentation] G-CSF promotes the proliferation of developing cardiomyocytes in vivo and in derivation from ES cells and iPS cells. (2010)
  • Author(s): Yuasa S
  • Organizer: World Stem Cells & Regenerative Medicine
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2010-10-27
• [Presentation] Inhibition of cardiac myocyte apoptosis by Zac1, an essential transcription factor for cardiac morphogeneis (2010)
  • Author(s): Yuasa S, Nishiyama T, Prall OJ, Harvey RP, Fukuda K
  • Organizer: Weinstein meeting
  • Place of Presentation: Amsterdam, Netherland
  • Year and Date: 2010-05-20
• [Book] ヒ トiPS細胞を用いた心筋再生医療の現状と未来 (2012)
  • Author(s): 湯浅慎介
  • Total Pages: 1
• [Book] 臨床応用へ向けたiPS細胞樹立方法の開発 (2012)
  • Author(s): 湯浅慎介
  • Total Pages: 5
• [Book] International Review of Thrombosis (2012)
  • Author(s): 湯浅慎介
  • Total Pages: 3
• [Book] 患者特異的iPS細胞による疾患メカニズムの解明、Annual Review 循環器 (2012)
  • Author(s): 湯浅慎介
  • Total Pages: 5
  • Publisher: 中外医学社
• [Book] iPS/ES細胞研究 (2011)
  • Author(s): 湯浅慎介
  • Total Pages: 1
  • Publisher: Medical Tribune
• [Book] 炎症細胞が作り出すニッチと筋肉再生 (2011)
  • Author(s): 湯浅慎介、福田恵一
  • Total Pages: 198-203
• [Book] 循環器疾患特異的iPS細胞研究の展開 (2011)
  • Author(s): 湯浅慎介、福田恵一
  • Total Pages: 10-13
• [Book] ヒト末梢血からのiPS細胞樹立方法の開発 (2010)
  • Author(s): 湯浅慎介
  • Total Pages: 1
  • Publisher: Medical View Point
• [Book] G-CSFを用いたES細胞、iPS細胞由来心筋前駆細胞の増殖促進 (2010)
  • Author(s): 湯浅慎介、福田恵一
  • Total Pages: 1258-1261