2012 Fiscal Year Final Research Report
Stem cell marker, nestin-targeting therapy in pancreatic cancer
| Project/Area Number |
22689038
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Nippon Medical School |
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Principal Investigator |
MATSUDA Yoko 日本医科大学, 医学部, 講師 (20363187)
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| Project Period (FY) |
2010 – 2012
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| Keywords | nestin / 膵癌 / 分子標的治療薬 / 浸潤 / 転移 / 癌幹細胞 |
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| Research Abstract |
In the present study, we have studied the effects and mechanisms of nestin-targeting therapy in pancreatic cancers. Inhibition of nestin in pancreatic cancer cell lines suppressed cell migration, invasion, sphere-forming ability and liver metastasis. The inhibitory effects of nestin were involved in the expression levels of F-actin, E-cadherin and transcription factor 4. We found that nestin expression was regulated by the methylation of the nestin gene and by the activation of cell signaling pathway proteins such as PKR and AKT. Tail vein injections of siRNA-targeting nestin into immunodeficient mice in which human pancreatic cancer had been transplanted suppressed both primary and metastatic tumor formation. Nestin is considered a promising target for pancreatic cancers, and further studies are needed to develop nestin-targeting therapy for pancreatic cancer.
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[Journal Article] Comparison of fixation methods for preservation of morphology, RNAs, and proteins from paraffin-embedded human cancer cell-implanted mouse models2011
Author(s)
Matsuda Y, Fujii T, Suzuki T, Yamahatsu K, Kawahara K, Teduka K, Kawamoto Y, Yamamoto T, Ishiwata T, Naito Z
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Journal Title
J Histochem Cytochem
Volume: 59
Pages: 68-75
DOI
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