2011 Fiscal Year Final Research Report
Functional analysis of Rest during the development of neuronal stem cells or neural crest cells
| Project/Area Number |
22700334
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neuroscience in general
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| Research Institution | Gifu University |
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Principal Investigator |
AOKI Hitomi 岐阜大学, 医学系研究科, 助教 (10550361)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 分子・細胞神経科学 / 発生神経科学 |
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| Research Abstract |
In the present study, we have generated Rest conditional knockout mice, which allow the effect of genetic ablation of Rest during embryonic neurogenesis to be examined in vivo. We show that Rest plays a role in suppressing the expression of neuronal genes in cultured neuronal cells in vitro, as well as in non-neuronal cells outside of the central nervous system, but that it is dispensable for embryonic neurogenesis in vivo. Our findings highlight the significance of extrinsic signals for the proper intrinsic regulation of neuronal gene expression levels in the specification of cell fate during embryonic neurogenesis in vivo. Rest is dispensable for the proper intrinsic regulations of neuronal gene expression in the specification of cell fate during neurogenesis in vivo
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