2012 Fiscal Year Final Research Report
Analysis of the neuropathological mechanism by using calpain mutant mice
| Project/Area Number |
22700379
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
TAKANO Jiro 独立行政法人理化学研究所, 神経蛋白制御研究チーム, 研究員 (60415213)
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| Project Period (FY) |
2010 – 2012
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| Keywords | カルパイン / プロテアーゼ / カルシウムシグナル |
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| Research Abstract |
In this study, we have analyzed the motor abnoromality in calpain-1 and calpain-2 double knockout mice. Neuron-specific deleted knockout showed abnormalities in motor and/or visual capacity in Morris water maze test. Then, we identified several substrates of calpain-1 and calpain-2, which relate the functional abnormalities, by masspectrometic technique and the iTRAQ method. However, some of the substrates were not cleaved so much by calpain. Thus, we are trying to identify calpain substrates in cell culture system using calcium ionophores.
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[Journal Article] Calpain-mediated ataxin-3 cleavage in the molecular pathogenesis of spinocerebellar ataxia type 3 (SCA3).2013
Author(s)
Hubener J, Weber JJ, Richter C, Honold L, Weiss A, Murad F, Breuer P, Wullner U, Bellstedt P, Paquet-Durand F, Takano J, Saido TC, Riess O, Nguyen HP.
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Journal Title
Hum Mol Genet.
Volume: 22(3)
Pages: 508-18
Peer Reviewed
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[Journal Article] Metabolic stress response implicated in diabetic retinopathy: the role of calpain, and the therapeutic impact of calpain inhibitor.2012
Author(s)
Shanab AY, Nakazawa T, Ryu M, Tanaka Y, Himori N, Taguchi K, Yasuda M, Watanabe R, Takano J, Saido T, Minegishi N, Miyata T, Abe T, Yamamoto M.
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Journal Title
Neurobiol Dis.
Volume: 48(3)
Pages: 556-67
Peer Reviewed
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[Journal Article] Mechanistic involvement of the calpain-calpastatin system in Alzheimer neuropathology.2012
Author(s)
Higuchi M, Iwata N, Matsuba Y, Takano J, Suemoto T, Maeda J, Ji B, Ono M, Staufenbiel M, Suhara T, Saido TC.
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Journal Title
FASEB J.
Volume: 26(3)
Pages: 1204-17
Peer Reviewed
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[Journal Article] Critical role of calpain in axonal damage-induced retinal ganglion cell death.2012
Author(s)
Ryu M, Yasuda M, Shi D, Shanab AY, Watanabe R, Himori N, Omodaka K, Yokoyama Y, Takano J, Saido T, Nakazawa T.
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Journal Title
J Neurosci Res.
Volume: 90(4)
Pages: 802-15
Peer Reviewed
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[Journal Article] Potent amyloidogenicity and pathogenicity of Aβ43.2011
Author(s)
Saito T, Suemoto T, Brouwers N, Sleegers K, Funamoto S, Mihira N, Matsuba Y, Yamada K, Nilsson P, Takano J, Nishimura M, Iwata N, Van Broeckhoven C, Ihara Y, Saido TC.
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Journal Title
Nat Neurosci.
Volume: 14(8)
Pages: 1023-32
Peer Reviewed
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[Journal Article] Calpastatin, an endogenous calpain-inhibitor protein, regulates the cleavage of the Cdk5 activator p35 to p25.2011
Author(s)
Sato K, Minegishi S, Takano J, Plattner F, Saito T, Asada A, Kawahara H, Iwata N, Saido TC, Hisanaga S.
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Journal Title
J Neurochem.
Volume: 117(3)
Pages: 504-15
Peer Reviewed
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