Functional analysis of novel NYAP family proteins

2011 Fiscal Year Final Research Report

• Project/Area Number: 22700391
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: The University of Tokyo
• Principal Investigator: YOKOYAMA Kazumasa 東京大学, 医科学研究所, 助教 (20431760)
• Project Period (FY): 2010 – 2011
• Keywords: 神経細胞 / チロシンキナーゼ / PI3K / WAVE

Research Abstract

The phosphoinositide 3-kinase(PI3K) pathway has been extensively studied in neuronal function and morphogenesis. However, the precise molecular mechanisms of PI3K activation and its downstream signalling in neurons remain elusive. In this study, we demonstrated that the NYAPs bridge a PI3K-WAVE1 association by simultaneously interacting with PI3K and the WAVE1 complex. Disruption of the NYAP genes in mice affects brain size and neurite elongation.

Research Products

• [Journal Article] Involvement of CNOT3 in mitotic progression through inhibition of MAD1 expression (2012)
  • Author(s): Takahashi A, Kikuguchi C, Morita M, Shimodaira T, Tokai-Nishizumi N, Yokoyama K, Ohsugi M, Suzuki T, and Yamamoto T.
  • Journal Title: Biochem Biophys Res Commun Volume: 419 Pages: 268-273
  • DOI: DOI:10.1016/j.bbrc.2012.02.007
• [Journal Article] Activation of Receptor Protein-Tyrosine Kinases from the Cytoplasmic Compartment (2012)
  • Author(s): Yamanashi Y, Tezuka T, and Yokoyama K.
  • Journal Title: J Biochem Volume: (n press)
  • DOI: DOI:10.1093/jb/mvs013
• [Journal Article] NYAP : a phosphoprotein family that links PI3K to WAVE1 signaling in neurons (2011)
  • Author(s): Yokoyama K, Tezuka T, Kotani M, Nakazawa T, Hoshina N, Shimoda Y, Kakuta S, Sudo K, Watanabe K, Iwakura Y, and Yamamoto T.
  • Journal Title: EMBO J Volume: 30 Pages: 4739-4754
  • DOI: DOI:10.1038/emboj.2011.348
• [Journal Article] Distinct expression patterns of the subunits of the CCR4-NOT deadenylase complex during neural development (2011)
  • Author(s): Chen C, Ito K, Takahashi A, Wang G, Suzuki T, Nakazawa T, Yamamoto T, and Yokoyama K.
  • Journal Title: Biochem Biophys Res Commun Volume: 411 Pages: 360-364
  • DOI: DOI:10.1016/j.bbrc.2011.06.148
• [Journal Article] CNOT2 depletion disrupts and inhibits the CCR4-NOT deadenylase complex and induces apoptotic cell death (2011)
  • Author(s): Ito K, Inoue T, Yokoyama K, Morita M, Suzuki T, and Yamamoto T.
  • Journal Title: Genes Cells Volume: 16 Pages: 368-379
  • DOI: DOI:10.1111/j.1365-2443.2011.01492.x
• [Journal Article] NMDAR2B tyrosine phosphorylation regulates anxiety-like behavior and CRF expression in the amygdala (2010)
  • Author(s): Delawary M, Tezuka T, Kiyama Y, Yokoyama K, Inoue T, Hattori S, Hashimoto R, Umemori H, Manabe T, Yamamoto T, and Nakazawa T.
  • Journal Title: Mol. Brain Volume: 3 Pages: 37
  • DOI: DOI:10.1186/1756-6606-3-37
• [Presentation] NYAPファミリーはContactinファミリー下流においてPI3K-Akt経路を活性化し神経突起伸長を促進する (2010)
  • Author(s): 横山一剛,山本雅
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Novel Phosphoprotein Family Activating the PI3K-Rac1-WAVE1 Signaling in Developing Neurons (2010)
  • Author(s): Yokoyama K, and Yamamoto T.
  • Organizer: Salk institute meeting(the 2010 Protein
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-08-18
• [Book] 実験医学増刊 (2012)
  • Author(s): 横山一剛,手塚徹,山梨裕司
  • Total Pages: 80-85
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www.ims.u-tokyo.ac.jp/frontier/,http://www.iam.u-tokyo.ac.jp/wakate/profile/ika_yokoyama.html6