2011 Fiscal Year Final Research Report
Regulatory mechanism of AMPA receptor dynamics by phospholipase A_2
| Project/Area Number |
22700405
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | 財団法人東京都医学総合研究所 (2011)
Tokyo Metropolitan Organization for Medical Research (2010) |
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Principal Investigator |
HIRABAYASHI Tetsuya 財団法人東京都医学総合研究所, 生体分子先端研究分野, 主席研究員 (90345025)
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| Project Period (FY) |
2010 – 2011
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| Keywords | 神経可塑性 / アラキドン酸 / 蛍光イメージング |
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| Research Abstract |
Trafficking of AMPA-type glutamate receptor subunit GluR2 in Purkinje neurons is a key mechanism to modulate long-term depression(LTD) in the cerebellum, which contributes to certain forms of motor learning and coordination. We have shown that full activation of cytosolic phospholipase A_2αand consequent liberation of arachidonic acid from membrane phospholipids are required for the prolonged decrease in the synaptic-surface expression of GluR2.To elucidate the regulatory mechanism of GluR2 dynamics by phospholipase A_2 and arachidonic acid, we attempted spatial and temporal control of local free arachidonic acid liberation using photolysis of caged compounds, visualization of internalized GluR2 movement, and comparative analysis of phospholipid composition.
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[Journal Article] Analysis of two major intracellular phospholipase A_2s in mast cells reveals crucial contribution of cPLA_2α, not iPLA_2β, to lipid mobilization in proximal mast cells and distal fibroblasts2011
Author(s)
Ueno N, Taketomi Y, Yamamoto K, Hirabayashi T, Kamei D, Kita Y, Shimizu T, Shinzawa K, Tsujimoto Y, Ikeda K, Taguchi R, Murakami M
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Journal Title
J Biol Chem
Volume: 286
Pages: 37249-63
DOI
Peer Reviewed
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