2011 Fiscal Year Final Research Report
Refilling rate of synaptic vesicles with glutamate in the mammalian presynaptic terminal
| Project/Area Number |
22700418
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Doshisha University |
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Principal Investigator |
HORI Tetsuya 同志社大学, 脳科学研究科, 嘱託研究員 (70396703)
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| Project Period (FY) |
2010 – 2011
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| Keywords | シナプス前終末 / グルタミン酸 / シナプス小胞 / Calyx of Held |
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| Research Abstract |
At the excitatory presynaptic terminal, we measured the rate of vesicle refilling with glutamate by rapidly increasing cytosolic glutamate concentration using caged compound photolysis, after depleting vesicular glutamate by whole-cell dialysis. Vesicle refilling rate, assessed from postsynaptic currents, had a time constant of about 15s at room temperature, and filled within 1 min for next exocytosis.
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