2012 Fiscal Year Final Research Report
Analysis of difference between female and male genome determined in pronuclear stage embryo.
| Project/Area Number |
22700456
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Tottori University |
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Principal Investigator |
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| Research Collaborator |
SOMA Atsumi 鳥取大学, 医学系研究科, 博士後期課程
OJI Asami 鳥取大学, 医学系研究科, 博士前期課程
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| Project Period (FY) |
2010 – 2012
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| Keywords | 雌雄前核 / DNA脱メチル化 / 受精卵 / Bisulfite sequencing / ゲノムインプリンティング / マイクロインジェクション / GFP |
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| Research Abstract |
In mammals, a fundamental function differs between maternaland paternal genomes. We found that exogenous DNA was controlled differently in its methylation status after injection into the female or male pronucleus. When H19 imprinting control region was used for exogenous DNA, its methylation level was higher in male pronucleus injection than that by female pronuleus injection. This result suggests that maternal and paternal specific epigeneteic marks in embryos may contribute in the establishment and maintenance of parental origin specific methylation of imprinting genesafter fertilization.
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