2012 Fiscal Year Final Research Report
Development of human antibody producing mice with human immune systems
| Project/Area Number |
22700458
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Central Institute for Experimental Animals |
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Principal Investigator |
ITO Ryoji 公益財団法人実験動物中央研究所, 免疫研究室, 研究員 (60425436)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ヒト化マウス / 疾患モデル / ヒト抗体 |
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| Research Abstract |
The purpose in this research is producing humanized mice reconstituted with human immune system. We obtained 2 strains of human gene transgenic (Tg) mice (HLA-DR4 Tg, IL-3/GM-CSF Tg), and human HSCs were transferred into them to analyze human immune responses in vivo. HLA-DR4 Tg mice showed the production of an antigen specific human IgG antibody and promotion of T cell activity. Meanwhile, differentiation of human mast cells and basophils were increased in hIL-3/GM Tg mice, and they could induce passive allergic response in vivo. Therefore, these humanized Tg mice may contribute to medical research such as the pre-clinical study on the development of new drug.
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[Journal Article] Osteosclerosis and inhibition of human hematopoiesis in NOG mice expressing human Delta-like 1 in osteoblasts2012
Author(s)
Ito R, Negishi N, Irie N, Matsuo K, Suzuki D, Katano I, Hayakawa E, Kawai K, Kamisako T, Eto T, Ogura T, Hozumi K, Ando K, Aiso S, Tamaoki N, Habu S, Ito M
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Journal Title
Experimental Hematology
Volume: 40
Pages: 953-963
DOI
Peer Reviewed
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