Spacial and temporal control of gene transfer for tissue engineering applications

2012 Fiscal Year Final Research Report

• Project/Area Number: 22700499
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biological material science
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: OYANE Ayako 独立行政法人産業技術総合研究所, ナノシステム研究部門, 主任研究員 (50356672)
• Project Period (FY): 2010 – 2012
• Keywords: 再生医療 / 遺伝子導入 / 細胞接着因子 / DNA / アパタイト複合層 / プラスミド / 遺伝子治療 / コーティング

Research Abstract

Recently, we developed a safe and efficient gene transfer techniqueusing DNA-cell adhesion molecule-apatite composite layers. In this study, we attempted to improve further this gene transfer technique and to perform feasibility assessment for tissue engi

Research Products

• [Journal Article] DNA-lipid-apatite composite layers enhance gene expression of mesenchymal stem cells (2013)
  • Author(s): X.P. Wang, A. Ito, X. Li, et al.
  • Journal Title: Mat. Sci. Eng. C Volume: 33 Pages: 512-518
  • DOI: DOI:10.1016/j.msec.2012.09.023
  • Peer Reviewed
• [Journal Article] Coprecipitation of DNA and calcium phosphate using an infusion fluid mixture (2013)
  • Author(s): A. Oyane, H. Araki, Y. Sogo, A. Ito, and H. Tsurushima
  • Journal Title: Key Eng. Mater Volume: 529-530 Pages: 465-470
  • DOI: DOI:10.4028/www.scientific.net/KEM.529-530.465
• [Journal Article] Fabrication of DNA - antibodyapatite composite layers for cell-targeted gene transfer (2012)
  • Author(s): Y. Yazaki, A. Oyane, H. Araki, et al.
  • Journal Title: Sci. Technol. Adv. Mater Volume: 13 Pages: 064204(8)
  • DOI: DOI:10.1088/1468-6996/13/6/064204
  • Peer Reviewed
• [Journal Article] リン酸カルシウム複合層を利用した遺伝子導入と再生医療応用 (2012)
  • Author(s): 大矢根 綾子、矢崎 侑振、鶴嶋 英夫他
  • Journal Title: 無機マテリアル学会会誌 2012 年度特集号 Volume: 19 Pages: 466-472
• [Journal Article] Calcium phosphate composite layers for surface-mediated gene transfer (2012)
  • Author(s): A. Oyane, X.P. Wang, Y. Sogo, A. Ito, and H. Tsurushima
  • Journal Title: Acta Biomater Volume: 8 Pages: 2034-2046
  • DOI: DOI:10.1016/j.actbio.2012.02.003
  • Peer Reviewed
• [Journal Article] Fabrication of a DNA-lipid-apatite composite layer for efficient and area-specific gene transfer (2012)
  • Author(s): A. Oyane, Y. Yazaki, H. Araki, et al.
  • Journal Title: J. Mater. Sci.: Mater. Med Volume: 23 Pages: 1011-1019
  • DOI: DOI:10.1007/s10856-012-4581-y
  • Peer Reviewed
• [Journal Article] BMP-2 and ALP gene expression induced by BMP-2gene-fibronectin-apatite composite layer (2011)
  • Author(s): X.P. Wang, A. Oyane, H. Tsurushima, Y. Sogo, X. Li, and A. Ito
  • Journal Title: Biomed. Mater. Volume: 6 Pages: 45004-45009
  • DOI: DOI:10.1088/1748-6041/6/4/045004
  • Peer Reviewed
• [Journal Article] Signal molecules-calcium phosphate coprecipitation and its biomedical application as a functional coating (2011)
  • Author(s): X.P. Wang, A. Ito, X. Li, Y. Sogo, and A. Oyane
  • Journal Title: Biofabrication Volume: 3 Pages: 22001-22011
  • DOI: DOI:10.1088/1758-5082/3/2/022001
  • Peer Reviewed
• [Journal Article] Control of gene transfer on DNA-fibronectin-apatite composite layer by incorporation of carbonate and fluoride ions (2011)
  • Author(s): Y. Yazaki, A. Oyane, Y. Sogo, A. Ito, A. Yamazaki, and H. Tsurushima
  • Journal Title: Biomater. Volume: 32 Pages: 4896-4902
  • DOI: DOI:10.1016/j.biomaterials.2011.03.021
  • Peer Reviewed
• [Journal Article] 溶液化学に立脚した有機-無機複合体の創製と医療応用 (2011)
  • Author(s): 大矢根綾子
  • Journal Title: バイオマテリアル -生体材料- Volume: 29 Pages: 35-40
  • URL: http://kokuhoken.net/jsbm/journal/past_29.html
• [Journal Article] Surface-mediated transfection のためのバイオセラミックスの設計と展開 (2010)
  • Author(s): 大矢根綾子 、 鶴嶋英夫、 伊藤敦夫
  • Journal Title: バイオマテリアル-生体材料- Volume: 28 Pages: 109-115
  • URL: http://kokuhoken.net/jsbm/journal/past_28.html
• [Journal Article] Spontaneous assembly ofDNA-amorphous calcium phosphate nanocomposite spheres for surface-mediated gene transfer
  • Author(s): A. Oyane, H. Araki, Y. Sogo, A. Ito, and H. Tsurushima
  • Journal Title: CrystEngComm Volume: (in press)
  • DOI: DOI:10.1039/C3CE40264A
  • Peer Reviewed
• [Journal Article] Coprecipitation of DNA-lipid complexes with apatite and comparison with superficial adsorption for gene transfer applications
  • Author(s): Y. Yazaki, A. Oyane, H. Tsurushima, et al.
  • Journal Title: J. Biomat. Appl. Volume: (in press)
  • DOI: DOI:10.1177/0885328213486706
  • Peer Reviewed
• [Presentation] Biomimetic calcium phosphate coating and application to gene delivery (2013)
  • Author(s): A. Oyane, Y. Yazaki, W . Zhang, et al., 8 人中
  • Organizer: 4th Asian Biomaterials Congress
  • Place of Presentation: Hong Kong (China)
  • Year and Date: 2013-06-26
• [Presentation] 遺伝子導入機能を有すアパタイト複合層の設計と応用 (2012)
  • Author(s): 大矢根綾子、矢崎侑振、王秀鵬他、
  • Organizer: 日本バイオマテリアル学会シンポジウム 2012
  • Place of Presentation: 仙台国際センター(宮城県)
  • Year and Date: 2012-11-27
• [Presentation] Signal molecule-apatite composite layers; from enhanced tissue regeneration to cancer immunotherapy (2012)
  • Author(s): A. Ito, Y. Sogo, A. Oyane, et al.
  • Organizer: 12th Asian BioCeramics Symposium (ABC2012)
  • Place of Presentation: National Cheng Kung University , Kuang-Fu Campus (Taiwan)
  • Year and Date: 2012-11-19
• [Presentation] リン酸カルシウム形成技術の開発とバイオメディカル応用 (2012)
  • Author(s): 大矢根 綾子
  • Organizer: 産総研オープンラボ講演会
  • Place of Presentation: 産業技術総合研究所つくばセンター(茨城県)
  • Year and Date: 2012-10-25
• [Presentation] Coprecipitation of DNA and calcium phosphate using an infusion fluid mixture (2012)
  • Author(s): A. Oyane, H. Araki, Y. Sogo, A. Ito, and H. Tsurushima
  • Organizer: 24rd International Symposium on Ceramics in Medicine (Bioceramics 24)
  • Place of Presentation: Fukuoka (Japan)
  • Year and Date: 2012-10-23
• [Presentation] Calcium phosphate composite coating for surface mediated non-viral gene transfer (2012)
  • Author(s): S. Bodhak, M. Kikuchi, A. Oyane, Y. Sogo, H. Tsurushima, and A. Ito
  • Organizer: Materials Science & Technology 2012
  • Place of Presentation: Pennsylvania (USA)
  • Year and Date: 2012-10-10
• [Presentation] 医療用輸液を用いた DNA-リン酸カルシウム複合層の形成と遺伝子導入応用 (2012)
  • Author(s): 大矢根綾子、荒木裕子、十河友、伊藤敦夫、鶴嶋英夫
  • Organizer: 日本セラミックス協会第25回秋季シンポジウム
  • Place of Presentation: 名古屋大学(愛知県)
  • Year and Date: 2012-09-21
• [Presentation] Effect of ion incorporation into a DNA-fibronectin-apatite composite layer on its gene transfer capability (2012)
  • Author(s): Y. Yushin, A. Oyane, A. Ito, et al.
  • Organizer: The 9th World Biomaterials Congress
  • Place of Presentation: Chengdu (China)
  • Year and Date: 2012-06-05
• [Presentation] A DNA-lipid-apatite composite layer mediates efficient and area-specificgene transfer (2012)
  • Author(s): A. Oyane, Y. Yushin, H. Araki, et al.
  • Organizer: The 9th W orld Biomaterials Congress
  • Place of Presentation: Chengdu(China)
  • Year and Date: 2012-06-03
• [Presentation] リン酸カルシウム形成技術の開発とバイオメディカル応用 (2012)
  • Author(s): 大矢根 綾子
  • Organizer: ナノテクノロジービジネス推進協議会NBCI「TIA-nano の見える化」第2回講演会
  • Place of Presentation: 日本教育会館(東京都)
  • Year and Date: 2012-02-29
• [Presentation] 抗体担持による DNA-アパタイト複合層の遺伝子導入能向上 (2012)
  • Author(s): 矢崎侑振、大矢根綾子、鶴嶋英夫, 他
  • Organizer: 医工連携フォーラム
  • Place of Presentation: 物質・材料研究機構(茨城県)
  • Year and Date: 2012-01-18
• [Presentation] Control of efficiency and timing of gene transfer on apatite-based composite layers (2011)
  • Author(s): A. Oyane, H. Tsurushima, Y. Yazaki, et al.
  • Organizer: International Conference on Biomaterials Science 2011 (ICBS2011)
  • Place of Presentation: Tsukuba International Congress Center (Ibaraki)
  • Year and Date: 20110316-17
• [Presentation] Bone engineering using BMP-2 gene-fibronectin-apatite composite layer (2011)
  • Author(s): H. Tsurushima, W. Zhang, A. Oyane, et al.
  • Organizer: International Conference on Biomaterials Science 2011 (ICBS2011)
  • Place of Presentation: Tsukuba International Congress Center (Ibaraki)
  • Year and Date: 20110316-17
• [Presentation] Antibody immobilized in a DNA-apatite composite layer enhances gene transfer efficiency (2011)
  • Author(s): Y. Yazaki, A. Oyane, H. Tsurushima, Y. Sogo, A. Ito, and A. Yamazaki
  • Organizer: 11th Asian BioCeramics Symposium(ABC2011)
  • Place of Presentation: National Institute for Materials Science (Ibaraki)
  • Year and Date: 2011-12-01
• [Presentation] Area-specific gene transfer on a DNA-Fibronectin-Apatite composite layer (2011)
  • Author(s): A. Oyane, Y. Yazaki, H. Tsurushima, Y. Sogo, A. Ito, and A. Yamazaki
  • Organizer: 11th Asian BioCeramics Symposium(ABC2011)
  • Place of Presentation: National Institute for Materials Science (Ibaraki)
  • Year and Date: 2011-11-30
• [Presentation] アパタイト複合層を利用した場所特異的遺伝子導入 (2011)
  • Author(s): 大矢根綾子、矢崎侑振、鶴嶋英夫、十河友、伊藤敦夫、山崎淳司
  • Organizer: つくば医工連携フォーラム 2011
  • Place of Presentation: 文部科学省研究交流センター(茨城県)
  • Year and Date: 2011-01-26
• [Presentation] DNA-adhesion molecule-apatite composite layers on biomaterial surfaces to promote tissue regeneration (2010)
  • Author(s): A. Oyane, H. Tsurushima, W. Zhang, Y . Sogo, A. Matsumura, and A. Ito
  • Organizer: 8th Korea-China Symposium on Biomaterials and Nano-bioTechnology
  • Place of Presentation: Pyeongchang (Korea)
  • Year and Date: 2010-12-18
• [Presentation] Bioactive molecules-apatite composite layers for tissue regeneration (2010)
  • Author(s): A. Ito, Y. Sogo, H. Mutsuzaki, et al.
  • Organizer: 5th International Symposium on Apatites and Correlative Biomaterials (ISACB2010)
  • Place of Presentation: Cairns Convention Center (Australia)
  • Year and Date: 2010-12-11
• [Presentation] BMP-2 遺伝子-フィブロネクチン-アパタイト複合層によるin vivo 骨組織再生 (2010)
  • Author(s): 大矢根綾子、Wei Zhang、十河友、伊藤敦夫、鶴嶋英夫
  • Organizer: 第32回日本バイオマテリアル学会大会
  • Place of Presentation: グランドプリンスホテル広島(広島県)
  • Year and Date: 2010-11-29
• [Presentation] Ectopic BMP-2 and ALP gene expression on a BMP-2 DNA-fibronectin-apatite composite layer (2010)
  • Author(s): 王秀鵬、大矢根綾子、鶴嶋英夫他
  • Organizer: 第32回日本バイオマテリアル学会大会
  • Place of Presentation: グランドプリンスホテル広島(広島県)
  • Year and Date: 2010-11-29
• [Presentation] 溶液化学に立脚した有機-無機複合体の創製と医療応用 (2010)
  • Author(s): 大矢根綾子
  • Organizer: 第32回日本バイオマテリアル学会大会
  • Place of Presentation: グランドプリンスホテル広島(広島県)
  • Year and Date: 2010-11-29
• [Presentation] BMP-2 遺伝子とフィブロネクチンを含むアパタイト層を持つ医療材料の開発-骨誘導機能性医療材料- (2010)
  • Author(s): 鶴嶋英夫、 Wei Zhang、 大矢根綾子、 十河友、伊藤敦夫、松村明
  • Organizer: 第69回日本脳神経外科学会学術総会
  • Place of Presentation: 福岡コンベンションセンター(福岡県)
  • Year and Date: 2010-10-27
• [Presentation] Highly efficient gene transfer system using laminin-DNA-apatite composite layer (2010)
  • Author(s): 鶴嶋英夫、大矢根綾子、伊藤敦夫、松村明
  • Organizer: 第16回日本遺伝子治療学会学術集会
  • Place of Presentation: 栃木県総合文化センター(栃木県)
  • Year and Date: 2010-07-01
• [Remarks] ホームページ
  • URL: http://staff.aist.go.jp/a-oyane/
• [Remarks] 大学女性協会守田科学研究奨励賞「バイオミネラリゼーションの科学と医療応用」大矢根綾子、2013年5月25日(予定)
• [Remarks] ABC Award (11th Asian BioCeramicsSymposium (ABC2011)), "Antibody immobilized in a DNA-apatite composite layer enhances gene transfer efficiency," 矢崎侑振、大矢根綾子、鶴嶋英夫、十河友、伊藤敦夫、山崎淳司、2011 年 12 月 2 日
• [Remarks] バイオマテリアル学会 バイオマテリアル科学奨励賞「溶液化学に立脚した有機-無機複合体の創製と医療応用」大矢根綾子、2010年11月29日
• [Patent(Industrial Property Rights)] 遺伝子導入用基材とその作製方法 (2012)
  • Inventor(s): 大矢根綾子、荒木裕子、伊藤敦夫、十河友、鶴嶋英夫、Subhadip Bodhak
  • Industrial Property Rights Holder: 大矢根綾子、荒木裕子、伊藤敦夫、十河友、鶴嶋英夫、Subhadip Bodhak
  • Industrial Property Number: 特願 2012-199854
  • Filing Date: 2012-09-11