2011 Fiscal Year Final Research Report

Isolation of peptides with antioxidant activity from biodiesel byproducts by autofocusing, which could be applied to the food system

Research Project

Project/Area Number	22700736
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Eating habits, studies on eating habits
Research Institution	Kyoto Prefectural University
Principal Investigator	PARK Eunyoung 京都府立大学, 食保健学科, 助手 (50405239)
Project Period (FY)	2010 – 2011
Keywords	食品と貯蔵 / 抗酸化 / ペプチド / 大量分画法
Research Abstract	Gluten and soy protein hydrolysates were fractionated on the basis of the amphoteric nature of sample peptides by autofocusing. Antioxidant activities of the autofocusing fractions were evaluated by DPPH and OH radical scavenging assays, ORAC assay, and so on. Also, the cooked pork patties added with peptides were stored at 4℃, 20℃in the dark. After incubation, lipid oxidation of the cooked pork patties was tested using TBA method. All autofocusing fractions of gluten hydrolysate and acidic, weak acidic fractions of soy protein hydrolysate suppressed oxidation of lipid in the patties.