2011 Fiscal Year Final Research Report
Oxidative stress signal function in stem cells via keap1-Nrf2 system
| Project/Area Number |
22700870
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor biology
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| Research Institution | Tohoku University |
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Principal Investigator |
MORITA Masanobu 東北大学, 大学院・医学系研究科, 助教 (10519094)
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| Project Period (FY) |
2010 – 2011
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| Keywords | がん微小環境 |
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| Research Abstract |
New ES cells lines are generated from Nrf2, Keap1 null blastocytes. These Nrf2 KO and Keap1 KO ES cells are expected to have phenotypes in oxidative stress responses. Nrf2 and Keap1 null blastocytes are obtained from cross mating heterozygous mutant mice. Genomic PCR are used to determine the genotypes of established ES cells. Nrf2 and Keap1 null ES cells show typical ES-like morphologies. The ES cell lines also show expression pattern of ES cell markers. Embryoid body formation are used as ES differentiation system.
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