2011 Fiscal Year Final Research Report
Screening for microRNA which is related to cancer progression by using novel microRNA inhibitory vectors
| Project/Area Number |
22700873
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Keywords | RNA干渉 / microRNA / inhibitor / がん |
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| Research Abstract |
The purpose of this project is improvement of miRNA inhibitory vector "TuD RNA ; Tough Decoy RNA" which we developed previously and search miRNAs that are related to cancer progression by using improved TuD RNA vector. In this project, we improved TuD RNA expression vector. We transduced improved TuD RNA expression lentivirus vector into cancer cells and observed that its miRNA inhibitory effects has been maintained for more than 100 days after transduction. Furthermore, improved-type TuD RNA expression lentivirus vector which targeted miR-200c induced epithelial-mesenchymal transition in cancer cells. These results showed that improved-type TuD RNA was a powerful tool for miRNA research.
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[Journal Article] Apotent 2'-0-methylated RNA-based micr RNA inhibitor with unique secondary structures2012
Author(s)
Haraguchi, T., Nakano, H., Tagawa, T., Ohki, T., Ueno, Y., Yoshida, T., and Iba, H
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Journal Title
DOI
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[Journal Article] Micro RNAs miR-199a-5p and-3p target the Brm subunit of SWI/SNF to generate a double-negative feedback loop in a variety of human cancers2011
Author(s)
Sakurai, K, Furukawa C, Haraguchi T, Inada K, Shiogama K, Tagawa T, Fujita S, Ueno Y, Ogata A, Ito M, Tsutsumi Y, Iba H
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Journal Title
Cancer Research
Volume: 71
Pages: 1680-1689
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