2012 Fiscal Year Final Research Report
Identification of new target proteins for metastasis suppressor protein Nm23-H1
| Project/Area Number |
22700883
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor biology
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| Research Institution | Kochi University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 胃癌 / 転移抑制 / Nm23-H1 / MALDI-TOFMS |
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| Research Abstract |
Nm23-H1 is known as the suppressor of tumor metastasis. To find the potential target of Nm23-H1 in epithelial cell, we used matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) analysis and N-terminal sequence analysis. We obtained 73 candidate proteins as a cellular target of Nm23-H1 including previous reported molecules. Those are categorized 4 groups which are involved in 8 cell structure proteins, 4 cell adhesion molecules, 20 signal molecules, and 12 cell constitution protein/unknown function molecules. One of candidatesspermatogenesis associated 13 (SPATA13) that is also known as adenomatous polyposis coli (APC)-stimulated guanine nucleotide exchange factor 2 (Asef2) observed in several tumors and associated with a decrease of cell to cell adhesion.
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