2012 Fiscal Year Final Research Report
Development of novel diagnosis of cancer treatment using fluorescence analysis and metabolomics.
| Project/Area Number |
22700907
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Tumor diagnosis
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| Research Institution | Fukuoka University |
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Principal Investigator |
ITOYAMA Miki 福岡大学, 薬学部, 助教 (60549690)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 癌 / 蛍光分析 / ポリアミン / メタボロミクス |
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| Research Abstract |
Aims to develop cancer therapy evaluation method based metabolomics, we have developed some fluorescence analysis methods of various metabolites are described below.(1) Polyamines analysis: To achieve simultaneous analysis of acetyl body and free form by excimer fluorescence derivatization.(2) Organic acids analysis: Where the FSD was developed by (Fluorous Scavengeing Derivatization) method, to analyze the organic acid of diabetes model mouse urine, and subjected to principal component analysis, to get the score plot that is different from the comparison group.(3) Amino acids analysis: Analysis of the healthy human blood amino acids by on-line pre-column derivatization with NBD-F reagent.(4) Peptides analysis: Achieved post-column analysis of Arg-containing peptides.
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